Human Lipoproteins at Model Cell Membranes: Effect of Lipoprotein Class on Lipid Exchange

Abstract High and low density lipoproteins (HDL and LDL) are thought to play vital roles in the onset and development of atherosclerosis; the biggest killer in the western world. Key issues of initial lipoprotein (LP) interactions at cellular membranes need to be addressed including LP deposition an...

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Autores principales:	K. L. Browning, T. K. Lind, S. Maric, S. Malekkhaiat-Häffner, G. N. Fredrikson, E. Bengtsson, M. Malmsten, M. Cárdenas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/997af01e86dd407b94b0f642cf3c4bf0
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spelling	oai:doaj.org-article:997af01e86dd407b94b0f642cf3c4bf02021-12-02T11:53:08ZHuman Lipoproteins at Model Cell Membranes: Effect of Lipoprotein Class on Lipid Exchange10.1038/s41598-017-07505-02045-2322https://doaj.org/article/997af01e86dd407b94b0f642cf3c4bf02017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07505-0https://doaj.org/toc/2045-2322Abstract High and low density lipoproteins (HDL and LDL) are thought to play vital roles in the onset and development of atherosclerosis; the biggest killer in the western world. Key issues of initial lipoprotein (LP) interactions at cellular membranes need to be addressed including LP deposition and lipid exchange. Here we present a protocol for monitoring the in situ kinetics of lipoprotein deposition and lipid exchange/removal at model cellular membranes using the non-invasive, surface sensitive methods of neutron reflection and quartz crystal microbalance with dissipation. For neutron reflection, lipid exchange and lipid removal can be distinguished thanks to the combined use of hydrogenated and tail-deuterated lipids. Both HDL and LDL remove lipids from the bilayer and deposit hydrogenated material into the lipid bilayer, however, the extent of removal and exchange depends on LP type. These results support the notion of HDL acting as the ‘good’ cholesterol, removing lipid material from lipid-loaded cells, whereas LDL acts as the ‘bad’ cholesterol, depositing lipid material into the vascular wall.K. L. BrowningT. K. LindS. MaricS. Malekkhaiat-HäffnerG. N. FredriksonE. BengtssonM. MalmstenM. CárdenasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q K. L. Browning T. K. Lind S. Maric S. Malekkhaiat-Häffner G. N. Fredrikson E. Bengtsson M. Malmsten M. Cárdenas Human Lipoproteins at Model Cell Membranes: Effect of Lipoprotein Class on Lipid Exchange
description	Abstract High and low density lipoproteins (HDL and LDL) are thought to play vital roles in the onset and development of atherosclerosis; the biggest killer in the western world. Key issues of initial lipoprotein (LP) interactions at cellular membranes need to be addressed including LP deposition and lipid exchange. Here we present a protocol for monitoring the in situ kinetics of lipoprotein deposition and lipid exchange/removal at model cellular membranes using the non-invasive, surface sensitive methods of neutron reflection and quartz crystal microbalance with dissipation. For neutron reflection, lipid exchange and lipid removal can be distinguished thanks to the combined use of hydrogenated and tail-deuterated lipids. Both HDL and LDL remove lipids from the bilayer and deposit hydrogenated material into the lipid bilayer, however, the extent of removal and exchange depends on LP type. These results support the notion of HDL acting as the ‘good’ cholesterol, removing lipid material from lipid-loaded cells, whereas LDL acts as the ‘bad’ cholesterol, depositing lipid material into the vascular wall.
format	article
author	K. L. Browning T. K. Lind S. Maric S. Malekkhaiat-Häffner G. N. Fredrikson E. Bengtsson M. Malmsten M. Cárdenas
author_facet	K. L. Browning T. K. Lind S. Maric S. Malekkhaiat-Häffner G. N. Fredrikson E. Bengtsson M. Malmsten M. Cárdenas
author_sort	K. L. Browning
title	Human Lipoproteins at Model Cell Membranes: Effect of Lipoprotein Class on Lipid Exchange
title_short	Human Lipoproteins at Model Cell Membranes: Effect of Lipoprotein Class on Lipid Exchange
title_full	Human Lipoproteins at Model Cell Membranes: Effect of Lipoprotein Class on Lipid Exchange
title_fullStr	Human Lipoproteins at Model Cell Membranes: Effect of Lipoprotein Class on Lipid Exchange
title_full_unstemmed	Human Lipoproteins at Model Cell Membranes: Effect of Lipoprotein Class on Lipid Exchange
title_sort	human lipoproteins at model cell membranes: effect of lipoprotein class on lipid exchange
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/997af01e86dd407b94b0f642cf3c4bf0
work_keys_str_mv	AT klbrowning humanlipoproteinsatmodelcellmembraneseffectoflipoproteinclassonlipidexchange AT tklind humanlipoproteinsatmodelcellmembraneseffectoflipoproteinclassonlipidexchange AT smaric humanlipoproteinsatmodelcellmembraneseffectoflipoproteinclassonlipidexchange AT smalekkhaiathaffner humanlipoproteinsatmodelcellmembraneseffectoflipoproteinclassonlipidexchange AT gnfredrikson humanlipoproteinsatmodelcellmembraneseffectoflipoproteinclassonlipidexchange AT ebengtsson humanlipoproteinsatmodelcellmembraneseffectoflipoproteinclassonlipidexchange AT mmalmsten humanlipoproteinsatmodelcellmembraneseffectoflipoproteinclassonlipidexchange AT mcardenas humanlipoproteinsatmodelcellmembraneseffectoflipoproteinclassonlipidexchange
_version_	1718394880757071872

Human Lipoproteins at Model Cell Membranes: Effect of Lipoprotein Class on Lipid Exchange

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