A potential probiotic Leuconostoc mesenteroides TBE-8 for honey bee

Abstract An isolated bacterium TBE-8, was identified as Leuconostoc mesenteroides according to the sequences of 16S rDNA and the 16S–23S rDNA intergenic spacer region. The probiotic properties of the L. mesenteroides TBE-8 strain were characterized and revealed that TBE-8 could utilize various carbo...

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Autores principales:	Yu-Han Huang, Yu-Hsin Chen, Jui-Hung Chen, Pei-Shou Hsu, Tzu-Hsien Wu, Chuen-Fu Lin, Chi-Chung Peng, Ming-Cheng Wu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/997d6675a41f4447beefee2d244085e7
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spelling	oai:doaj.org-article:997d6675a41f4447beefee2d244085e72021-12-02T15:15:58ZA potential probiotic Leuconostoc mesenteroides TBE-8 for honey bee10.1038/s41598-021-97950-92045-2322https://doaj.org/article/997d6675a41f4447beefee2d244085e72021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97950-9https://doaj.org/toc/2045-2322Abstract An isolated bacterium TBE-8, was identified as Leuconostoc mesenteroides according to the sequences of 16S rDNA and the 16S–23S rDNA intergenic spacer region. The probiotic properties of the L. mesenteroides TBE-8 strain were characterized and revealed that TBE-8 could utilize various carbohydrates, exhibited high tolerance to sucrose’s osmotic pressure and acidic conditions, and could mitigate the impact of the bee pathogen Paenibacillus larvae. In addition, we found that the TBE-8 broth increased the expression of the nutrition-related genes major royal jelly protein 1 and vitellogenin in bees by approximately 1400- and 20-fold, respectively. The expression of genes encoding two antibacterial peptides, hymenoptaecin and apidaecin, in the bee abdomen was significantly increased by 17- and 7-fold in bees fed with the TBE-8 fermented broth. Furthermore, we fed four-frame bee colonies with 50% sucrose syrup containing TBE-8 and can detect the presence of approximately 2 × 106 16S rDNA copies of TBE-8 in the guts of all bees in 24 h, and the retention of TBE-8 in the bee gut for at least 5 days. These findings indicate that the L. mesenteroides TBE-8 has high potential as a bee probiotic and could enhance the health of bee colonies.Yu-Han HuangYu-Hsin ChenJui-Hung ChenPei-Shou HsuTzu-Hsien WuChuen-Fu LinChi-Chung PengMing-Cheng WuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yu-Han Huang Yu-Hsin Chen Jui-Hung Chen Pei-Shou Hsu Tzu-Hsien Wu Chuen-Fu Lin Chi-Chung Peng Ming-Cheng Wu A potential probiotic Leuconostoc mesenteroides TBE-8 for honey bee
description	Abstract An isolated bacterium TBE-8, was identified as Leuconostoc mesenteroides according to the sequences of 16S rDNA and the 16S–23S rDNA intergenic spacer region. The probiotic properties of the L. mesenteroides TBE-8 strain were characterized and revealed that TBE-8 could utilize various carbohydrates, exhibited high tolerance to sucrose’s osmotic pressure and acidic conditions, and could mitigate the impact of the bee pathogen Paenibacillus larvae. In addition, we found that the TBE-8 broth increased the expression of the nutrition-related genes major royal jelly protein 1 and vitellogenin in bees by approximately 1400- and 20-fold, respectively. The expression of genes encoding two antibacterial peptides, hymenoptaecin and apidaecin, in the bee abdomen was significantly increased by 17- and 7-fold in bees fed with the TBE-8 fermented broth. Furthermore, we fed four-frame bee colonies with 50% sucrose syrup containing TBE-8 and can detect the presence of approximately 2 × 106 16S rDNA copies of TBE-8 in the guts of all bees in 24 h, and the retention of TBE-8 in the bee gut for at least 5 days. These findings indicate that the L. mesenteroides TBE-8 has high potential as a bee probiotic and could enhance the health of bee colonies.
format	article
author	Yu-Han Huang Yu-Hsin Chen Jui-Hung Chen Pei-Shou Hsu Tzu-Hsien Wu Chuen-Fu Lin Chi-Chung Peng Ming-Cheng Wu
author_facet	Yu-Han Huang Yu-Hsin Chen Jui-Hung Chen Pei-Shou Hsu Tzu-Hsien Wu Chuen-Fu Lin Chi-Chung Peng Ming-Cheng Wu
author_sort	Yu-Han Huang
title	A potential probiotic Leuconostoc mesenteroides TBE-8 for honey bee
title_short	A potential probiotic Leuconostoc mesenteroides TBE-8 for honey bee
title_full	A potential probiotic Leuconostoc mesenteroides TBE-8 for honey bee
title_fullStr	A potential probiotic Leuconostoc mesenteroides TBE-8 for honey bee
title_full_unstemmed	A potential probiotic Leuconostoc mesenteroides TBE-8 for honey bee
title_sort	potential probiotic leuconostoc mesenteroides tbe-8 for honey bee
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/997d6675a41f4447beefee2d244085e7
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A potential probiotic Leuconostoc mesenteroides TBE-8 for honey bee

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