NK Cells Can Preferentially Target Prostate Cancer Stem-like Cells via the TRAIL/DR5 Signaling Pathway

Background: The occurrence of androgen-dependent prostate cancer mainly depends on prostate cancer stem cells. To reduce the risk of androgen-dependent prostate cancer, the direct elimination of prostate cancer stem cells is important, but an elimination strategy has not yet been established. A prev...

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Autores principales:	Taiga Seki, Yui Shimizu, Kyota Ishii, Yuzuki Takahama, Kazunori Kato, Tomohiro Yano
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	androgen-dependent prostate cancer stem cells NK cells cytotoxicity TRAIL/DR5 signal pathway Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/997fb96bdb004b629302cfe1b5a2b71e
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spelling	oai:doaj.org-article:997fb96bdb004b629302cfe1b5a2b71e2021-11-25T16:53:58ZNK Cells Can Preferentially Target Prostate Cancer Stem-like Cells via the TRAIL/DR5 Signaling Pathway10.3390/biom111117022218-273Xhttps://doaj.org/article/997fb96bdb004b629302cfe1b5a2b71e2021-11-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1702https://doaj.org/toc/2218-273XBackground: The occurrence of androgen-dependent prostate cancer mainly depends on prostate cancer stem cells. To reduce the risk of androgen-dependent prostate cancer, the direct elimination of prostate cancer stem cells is important, but an elimination strategy has not yet been established. A previous study showed that natural killer (NK) cells can preferentially target cancer stem cells in several solid tumors except prostate cancer. In this context, this study was undertaken to investigate if NK cells can selectively attack androgen-dependent prostate cancer stem cells. Methods: Prostate cancer stem-like cells were separated from an androgen-dependent prostate cancer cell line (LNCaP) using a three-dimensional culture system. LNCaP stem-like cells or LNCaP cells were co-cultured with human NK cells (KHYG-1) for 24–72 h, and cell viability was determined using the WST-8 method. The expression of each protein in the cell membrane was evaluated through FACS analysis, and mRNA levels were determined using real-time PCR. Results: KHYG-1 cells had more potent cytotoxicity against LNCaP stem-like cells than LNCaP cells, and the potency of the cytotoxicity was strongly related to the TRAIL/DR5 cell death pathway. Conclusion: NK cells can preferentially target prostate cancer stem-like cells via the TRAIL/DR5 pathway.Taiga SekiYui ShimizuKyota IshiiYuzuki TakahamaKazunori KatoTomohiro YanoMDPI AGarticleandrogen-dependent prostate cancerstem cellsNK cellscytotoxicityTRAIL/DR5 signal pathwayMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1702, p 1702 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	androgen-dependent prostate cancer stem cells NK cells cytotoxicity TRAIL/DR5 signal pathway Microbiology QR1-502
spellingShingle	androgen-dependent prostate cancer stem cells NK cells cytotoxicity TRAIL/DR5 signal pathway Microbiology QR1-502 Taiga Seki Yui Shimizu Kyota Ishii Yuzuki Takahama Kazunori Kato Tomohiro Yano NK Cells Can Preferentially Target Prostate Cancer Stem-like Cells via the TRAIL/DR5 Signaling Pathway
description	Background: The occurrence of androgen-dependent prostate cancer mainly depends on prostate cancer stem cells. To reduce the risk of androgen-dependent prostate cancer, the direct elimination of prostate cancer stem cells is important, but an elimination strategy has not yet been established. A previous study showed that natural killer (NK) cells can preferentially target cancer stem cells in several solid tumors except prostate cancer. In this context, this study was undertaken to investigate if NK cells can selectively attack androgen-dependent prostate cancer stem cells. Methods: Prostate cancer stem-like cells were separated from an androgen-dependent prostate cancer cell line (LNCaP) using a three-dimensional culture system. LNCaP stem-like cells or LNCaP cells were co-cultured with human NK cells (KHYG-1) for 24–72 h, and cell viability was determined using the WST-8 method. The expression of each protein in the cell membrane was evaluated through FACS analysis, and mRNA levels were determined using real-time PCR. Results: KHYG-1 cells had more potent cytotoxicity against LNCaP stem-like cells than LNCaP cells, and the potency of the cytotoxicity was strongly related to the TRAIL/DR5 cell death pathway. Conclusion: NK cells can preferentially target prostate cancer stem-like cells via the TRAIL/DR5 pathway.
format	article
author	Taiga Seki Yui Shimizu Kyota Ishii Yuzuki Takahama Kazunori Kato Tomohiro Yano
author_facet	Taiga Seki Yui Shimizu Kyota Ishii Yuzuki Takahama Kazunori Kato Tomohiro Yano
author_sort	Taiga Seki
title	NK Cells Can Preferentially Target Prostate Cancer Stem-like Cells via the TRAIL/DR5 Signaling Pathway
title_short	NK Cells Can Preferentially Target Prostate Cancer Stem-like Cells via the TRAIL/DR5 Signaling Pathway
title_full	NK Cells Can Preferentially Target Prostate Cancer Stem-like Cells via the TRAIL/DR5 Signaling Pathway
title_fullStr	NK Cells Can Preferentially Target Prostate Cancer Stem-like Cells via the TRAIL/DR5 Signaling Pathway
title_full_unstemmed	NK Cells Can Preferentially Target Prostate Cancer Stem-like Cells via the TRAIL/DR5 Signaling Pathway
title_sort	nk cells can preferentially target prostate cancer stem-like cells via the trail/dr5 signaling pathway
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/997fb96bdb004b629302cfe1b5a2b71e
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NK Cells Can Preferentially Target Prostate Cancer Stem-like Cells via the TRAIL/DR5 Signaling Pathway

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