Development of an indirect enzyme-linked immunosorbent assay for the detection of osmotolerant yeast Zygosaccharomyces rouxii in different food

A polyclonal antibody-based indirect ELISA for the sensitive and specific detection of osmotolerant Zygosaccharomyces rouxii was established. The reactivity of the polyclonal antibodies (pAbs) with Z. rouxii was confirmed by immunofluorescence. The ELISA detection limit was 5 × 104 CFU/mL. For an ev...

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Autores principales:	Hong Guo, Ya-Hong Yuan, Chen Niu, Yue Qiu, JianPing Wei, Tian-Li Yue
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	zygosaccharomyces rouxii polyclonal antibodies indirect elisa immuno-detection food quality Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/998bb70dd56f41ea91f438385b63aece
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spelling	oai:doaj.org-article:998bb70dd56f41ea91f438385b63aece2021-11-26T11:19:46ZDevelopment of an indirect enzyme-linked immunosorbent assay for the detection of osmotolerant yeast Zygosaccharomyces rouxii in different food0954-01051465-344310.1080/09540105.2018.1491532https://doaj.org/article/998bb70dd56f41ea91f438385b63aece2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1491532https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443A polyclonal antibody-based indirect ELISA for the sensitive and specific detection of osmotolerant Zygosaccharomyces rouxii was established. The reactivity of the polyclonal antibodies (pAbs) with Z. rouxii was confirmed by immunofluorescence. The ELISA detection limit was 5 × 104 CFU/mL. For an evaluation of the specificity of pAbs, reactivity to 10 fungi species and 2 bacteria species commonly found in food environments was analysed by indirect ELISA. ELISA reacts with all tested Z. rouxii, whereas no cross-reactions or weakly reactions with 9 fungi and 2 bacteria were observed. The immunoassay staining confirmed the ELISA. Further on, the assay applicability was tested with concentrated apple juice, wine, and honey. Food samples were inoculated with 100 CFU/mL of Z. rouxii. After enrichment for 24 h, the established ELISA can successfully detect contaminated wine and concentrated apple juice samples, while only 30 h was required for honey samples.Hong GuoYa-Hong YuanChen NiuYue QiuJianPing WeiTian-Li YueTaylor & Francis Grouparticlezygosaccharomyces rouxiipolyclonal antibodiesindirect elisaimmuno-detectionfood qualityAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 976-988 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	zygosaccharomyces rouxii polyclonal antibodies indirect elisa immuno-detection food quality Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	zygosaccharomyces rouxii polyclonal antibodies indirect elisa immuno-detection food quality Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Hong Guo Ya-Hong Yuan Chen Niu Yue Qiu JianPing Wei Tian-Li Yue Development of an indirect enzyme-linked immunosorbent assay for the detection of osmotolerant yeast Zygosaccharomyces rouxii in different food
description	A polyclonal antibody-based indirect ELISA for the sensitive and specific detection of osmotolerant Zygosaccharomyces rouxii was established. The reactivity of the polyclonal antibodies (pAbs) with Z. rouxii was confirmed by immunofluorescence. The ELISA detection limit was 5 × 104 CFU/mL. For an evaluation of the specificity of pAbs, reactivity to 10 fungi species and 2 bacteria species commonly found in food environments was analysed by indirect ELISA. ELISA reacts with all tested Z. rouxii, whereas no cross-reactions or weakly reactions with 9 fungi and 2 bacteria were observed. The immunoassay staining confirmed the ELISA. Further on, the assay applicability was tested with concentrated apple juice, wine, and honey. Food samples were inoculated with 100 CFU/mL of Z. rouxii. After enrichment for 24 h, the established ELISA can successfully detect contaminated wine and concentrated apple juice samples, while only 30 h was required for honey samples.
format	article
author	Hong Guo Ya-Hong Yuan Chen Niu Yue Qiu JianPing Wei Tian-Li Yue
author_facet	Hong Guo Ya-Hong Yuan Chen Niu Yue Qiu JianPing Wei Tian-Li Yue
author_sort	Hong Guo
title	Development of an indirect enzyme-linked immunosorbent assay for the detection of osmotolerant yeast Zygosaccharomyces rouxii in different food
title_short	Development of an indirect enzyme-linked immunosorbent assay for the detection of osmotolerant yeast Zygosaccharomyces rouxii in different food
title_full	Development of an indirect enzyme-linked immunosorbent assay for the detection of osmotolerant yeast Zygosaccharomyces rouxii in different food
title_fullStr	Development of an indirect enzyme-linked immunosorbent assay for the detection of osmotolerant yeast Zygosaccharomyces rouxii in different food
title_full_unstemmed	Development of an indirect enzyme-linked immunosorbent assay for the detection of osmotolerant yeast Zygosaccharomyces rouxii in different food
title_sort	development of an indirect enzyme-linked immunosorbent assay for the detection of osmotolerant yeast zygosaccharomyces rouxii in different food
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/998bb70dd56f41ea91f438385b63aece
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Development of an indirect enzyme-linked immunosorbent assay for the detection of osmotolerant yeast Zygosaccharomyces rouxii in different food

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