Role of Beetroot (Beta vulgaris) Juice on Chronic Nanotoxicity of Silver Nanoparticle-Induced Hepatotoxicity in Male Rats

Tarfa Albrahim,1 Mona A Alonazi2 1College of Health and Rehabilitation Sciences, Department of Health Sciences, Clinical Nutrition, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia; 2Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi ArabiaCorrespond...

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Autores principales:	Albrahim T, Alonazi MA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	silver nanoparticles beta vulgaris oxidative stress p53 and bcl-2 expression hepatotoxicity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/99925e9a01f04b9291f0d5d7d6f9d5e7
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spelling	oai:doaj.org-article:99925e9a01f04b9291f0d5d7d6f9d5e72021-12-02T09:14:38ZRole of Beetroot (Beta vulgaris) Juice on Chronic Nanotoxicity of Silver Nanoparticle-Induced Hepatotoxicity in Male Rats1178-2013https://doaj.org/article/99925e9a01f04b9291f0d5d7d6f9d5e72020-05-01T00:00:00Zhttps://www.dovepress.com/role-of-beetroot-beta-vulgaris-juice-on-chronic-nanotoxicity-of-silver-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Tarfa Albrahim,1 Mona A Alonazi2 1College of Health and Rehabilitation Sciences, Department of Health Sciences, Clinical Nutrition, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia; 2Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi ArabiaCorrespondence: Tarfa Albrahim Email tialbarhim@pnu.edu.saIntroduction: Nanoparticles are at the forefront of rapidly developing nanotechnology and have gained much attention for their application as an effective drug delivery system and as a mediated therapeutic agent for cancer. However, the cytotoxicity of nanoparticles is still relatively unknown and, therefore, additional study is required in order to elucidate the potential toxicity of these nanoparticles on cells.Materials and Methods: Thus, the following work aimed to investigate the capability of Beta vulgaris (beetroot) water extract (BWE; 200 mg/kg) to protect hepatic tissue following silver nanoparticles (AgNPs; 80 mg/kg; > 100 nm) intoxication in male rats.Results: AgNPs-intoxication elevated the liver function markers – including serum transaminases and alkaline phosphatase activities – and decreased serum levels of albumin and total proteins, in addition to disturbing the oxidation homeostasis. This is evidenced by the increased lipid peroxidation, the depleted glutathione, and the suppressed activity of superoxide dismutase and catalase. In addition, an apoptotic reaction was observed following AgNPs treatment, as indicated by the up-regulation of p53 and down-regulating Bcl-2 expressions, examined by the immunohistochemistry method. Furthermore, AgNPs exhibited a marked elevation in liver DNA damage that was indicated by an increase in tail length, tail DNA% and tail movement. However, BWE eliminated the biochemical and histological alterations, reflecting its hepatoprotection effect in response to AgNPs.Discussion: Collectively, the present data suggest that BWE could be used following AgNPs as a potential therapeutic intervention to minimize AgNPs-induced liver toxicity.Keywords: silver nanoparticles, Beta vulgaris, oxidative stress, p53 and Bcl-2 expression, hepatotoxicityAlbrahim TAlonazi MADove Medical Pressarticlesilver nanoparticlesbeta vulgarisoxidative stressp53 and bcl-2 expressionhepatotoxicityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 3471-3482 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	silver nanoparticles beta vulgaris oxidative stress p53 and bcl-2 expression hepatotoxicity Medicine (General) R5-920
spellingShingle	silver nanoparticles beta vulgaris oxidative stress p53 and bcl-2 expression hepatotoxicity Medicine (General) R5-920 Albrahim T Alonazi MA Role of Beetroot (Beta vulgaris) Juice on Chronic Nanotoxicity of Silver Nanoparticle-Induced Hepatotoxicity in Male Rats
description	Tarfa Albrahim,1 Mona A Alonazi2 1College of Health and Rehabilitation Sciences, Department of Health Sciences, Clinical Nutrition, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia; 2Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi ArabiaCorrespondence: Tarfa Albrahim Email tialbarhim@pnu.edu.saIntroduction: Nanoparticles are at the forefront of rapidly developing nanotechnology and have gained much attention for their application as an effective drug delivery system and as a mediated therapeutic agent for cancer. However, the cytotoxicity of nanoparticles is still relatively unknown and, therefore, additional study is required in order to elucidate the potential toxicity of these nanoparticles on cells.Materials and Methods: Thus, the following work aimed to investigate the capability of Beta vulgaris (beetroot) water extract (BWE; 200 mg/kg) to protect hepatic tissue following silver nanoparticles (AgNPs; 80 mg/kg; > 100 nm) intoxication in male rats.Results: AgNPs-intoxication elevated the liver function markers – including serum transaminases and alkaline phosphatase activities – and decreased serum levels of albumin and total proteins, in addition to disturbing the oxidation homeostasis. This is evidenced by the increased lipid peroxidation, the depleted glutathione, and the suppressed activity of superoxide dismutase and catalase. In addition, an apoptotic reaction was observed following AgNPs treatment, as indicated by the up-regulation of p53 and down-regulating Bcl-2 expressions, examined by the immunohistochemistry method. Furthermore, AgNPs exhibited a marked elevation in liver DNA damage that was indicated by an increase in tail length, tail DNA% and tail movement. However, BWE eliminated the biochemical and histological alterations, reflecting its hepatoprotection effect in response to AgNPs.Discussion: Collectively, the present data suggest that BWE could be used following AgNPs as a potential therapeutic intervention to minimize AgNPs-induced liver toxicity.Keywords: silver nanoparticles, Beta vulgaris, oxidative stress, p53 and Bcl-2 expression, hepatotoxicity
format	article
author	Albrahim T Alonazi MA
author_facet	Albrahim T Alonazi MA
author_sort	Albrahim T
title	Role of Beetroot (Beta vulgaris) Juice on Chronic Nanotoxicity of Silver Nanoparticle-Induced Hepatotoxicity in Male Rats
title_short	Role of Beetroot (Beta vulgaris) Juice on Chronic Nanotoxicity of Silver Nanoparticle-Induced Hepatotoxicity in Male Rats
title_full	Role of Beetroot (Beta vulgaris) Juice on Chronic Nanotoxicity of Silver Nanoparticle-Induced Hepatotoxicity in Male Rats
title_fullStr	Role of Beetroot (Beta vulgaris) Juice on Chronic Nanotoxicity of Silver Nanoparticle-Induced Hepatotoxicity in Male Rats
title_full_unstemmed	Role of Beetroot (Beta vulgaris) Juice on Chronic Nanotoxicity of Silver Nanoparticle-Induced Hepatotoxicity in Male Rats
title_sort	role of beetroot (beta vulgaris) juice on chronic nanotoxicity of silver nanoparticle-induced hepatotoxicity in male rats
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/99925e9a01f04b9291f0d5d7d6f9d5e7
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Role of Beetroot (Beta vulgaris) Juice on Chronic Nanotoxicity of Silver Nanoparticle-Induced Hepatotoxicity in Male Rats

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