Equivalent SARS-CoV-2 viral loads by PCR between nasopharyngeal swab and saliva in symptomatic patients

Abstract Emerging evidences have shown the utility of saliva for the detection of SARS-CoV-2 by PCR as alternative to nasopharyngeal swab (NPS). However, conflicting results have been reported regarding viral loads between NPS and saliva. We conducted a study to compare the viral loads between NPS a...

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Autores principales:	Isao Yokota, Takeshi Hattori, Peter Y. Shane, Satoshi Konno, Atsushi Nagasaka, Kimihiro Takeyabu, Shinichi Fujisawa, Mutsumi Nishida, Takanori Teshima
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9995bc5a4cf5429d95f710a191b54626
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spelling	oai:doaj.org-article:9995bc5a4cf5429d95f710a191b546262021-12-02T13:20:14ZEquivalent SARS-CoV-2 viral loads by PCR between nasopharyngeal swab and saliva in symptomatic patients10.1038/s41598-021-84059-22045-2322https://doaj.org/article/9995bc5a4cf5429d95f710a191b546262021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84059-2https://doaj.org/toc/2045-2322Abstract Emerging evidences have shown the utility of saliva for the detection of SARS-CoV-2 by PCR as alternative to nasopharyngeal swab (NPS). However, conflicting results have been reported regarding viral loads between NPS and saliva. We conducted a study to compare the viral loads between NPS and saliva in 42 COVID-19 patients. Viral loads were estimated by the cycle threshold (Ct) values. SARS-CoV-2 was detected in 34 (81%) using NPS with median Ct value of 27.4, and 38 (90%) using saliva with median Ct value of 28.9 (P = 0.79). Kendall’s W was 0.82, showing a high degree of agreement, indicating equivalent viral loads in NPS and saliva. After symptom onset, the Ct values of both NPS and saliva continued to increase over time, with no substantial difference. Self-collected saliva has a detection sensitivity comparable to that of NPS and is a useful diagnostic tool with mitigating uncomfortable process and the risk of aerosol transmission to healthcare workers.Isao YokotaTakeshi HattoriPeter Y. ShaneSatoshi KonnoAtsushi NagasakaKimihiro TakeyabuShinichi FujisawaMutsumi NishidaTakanori TeshimaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-4 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Isao Yokota Takeshi Hattori Peter Y. Shane Satoshi Konno Atsushi Nagasaka Kimihiro Takeyabu Shinichi Fujisawa Mutsumi Nishida Takanori Teshima Equivalent SARS-CoV-2 viral loads by PCR between nasopharyngeal swab and saliva in symptomatic patients
description	Abstract Emerging evidences have shown the utility of saliva for the detection of SARS-CoV-2 by PCR as alternative to nasopharyngeal swab (NPS). However, conflicting results have been reported regarding viral loads between NPS and saliva. We conducted a study to compare the viral loads between NPS and saliva in 42 COVID-19 patients. Viral loads were estimated by the cycle threshold (Ct) values. SARS-CoV-2 was detected in 34 (81%) using NPS with median Ct value of 27.4, and 38 (90%) using saliva with median Ct value of 28.9 (P = 0.79). Kendall’s W was 0.82, showing a high degree of agreement, indicating equivalent viral loads in NPS and saliva. After symptom onset, the Ct values of both NPS and saliva continued to increase over time, with no substantial difference. Self-collected saliva has a detection sensitivity comparable to that of NPS and is a useful diagnostic tool with mitigating uncomfortable process and the risk of aerosol transmission to healthcare workers.
format	article
author	Isao Yokota Takeshi Hattori Peter Y. Shane Satoshi Konno Atsushi Nagasaka Kimihiro Takeyabu Shinichi Fujisawa Mutsumi Nishida Takanori Teshima
author_facet	Isao Yokota Takeshi Hattori Peter Y. Shane Satoshi Konno Atsushi Nagasaka Kimihiro Takeyabu Shinichi Fujisawa Mutsumi Nishida Takanori Teshima
author_sort	Isao Yokota
title	Equivalent SARS-CoV-2 viral loads by PCR between nasopharyngeal swab and saliva in symptomatic patients
title_short	Equivalent SARS-CoV-2 viral loads by PCR between nasopharyngeal swab and saliva in symptomatic patients
title_full	Equivalent SARS-CoV-2 viral loads by PCR between nasopharyngeal swab and saliva in symptomatic patients
title_fullStr	Equivalent SARS-CoV-2 viral loads by PCR between nasopharyngeal swab and saliva in symptomatic patients
title_full_unstemmed	Equivalent SARS-CoV-2 viral loads by PCR between nasopharyngeal swab and saliva in symptomatic patients
title_sort	equivalent sars-cov-2 viral loads by pcr between nasopharyngeal swab and saliva in symptomatic patients
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9995bc5a4cf5429d95f710a191b54626
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Equivalent SARS-CoV-2 viral loads by PCR between nasopharyngeal swab and saliva in symptomatic patients

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