Long non-coding RNA NBR2 suppresses the progress of colorectal cancer in vitro and in vivo by regulating the polarization of TAM

Colorectal cancer (CRC) threatens the health of patients with high mortality, which lacks sensitive biomarkers for diagnosis to improve total survival. The lncRNA NBR2 is reported to be downregulated in CRC and suppresses the proliferation of CRC cells. However, the underlying mechanisms remain uncl...

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Autores principales:	Fuji Lai, Huiqin Zhang, Binbin Xu, Yangyang Xie, Hua Yu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	lncrna nbr 2 colorectal cancer tumor associated macrophage polarization Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/999f84986c3e4be89b81ff21b95a7c5f
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spelling	oai:doaj.org-article:999f84986c3e4be89b81ff21b95a7c5f2021-11-17T14:21:59ZLong non-coding RNA NBR2 suppresses the progress of colorectal cancer in vitro and in vivo by regulating the polarization of TAM2165-59792165-598710.1080/21655979.2021.1958558https://doaj.org/article/999f84986c3e4be89b81ff21b95a7c5f2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1958558https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Colorectal cancer (CRC) threatens the health of patients with high mortality, which lacks sensitive biomarkers for diagnosis to improve total survival. The lncRNA NBR2 is reported to be downregulated in CRC and suppresses the proliferation of CRC cells. However, the underlying mechanisms remain unclear. The present study aimed to explore the regulatory function of the lncRNA NBR2 on tumor-associated macrophage (TAM) polarization and its consequent anti-tumor effect. Two CRC cell lines were used in this study. We found that the lncRNA NBR2, TNF-α, and HLA-DR were downregulated, and Arg-1, CD163, CD206, and IL-4 were upregulated in CRC tumors. M1 polarization was activated and M2 polarization was suppressed in NBR2-overexpressed macrophages, accompanied by increased production of inflammatory factors, decreased proliferation, and inhibited migration ability in the co-culture system of HCT-116 cells (SW480 cells) and NBR2-overexpressed macrophages. The promoted proliferation and migration were observed in the co-culture system of HCT-116 cells (SW480 cells) and NBR2-knockdown macrophages. The tumor growth of both HCT-116 cells and SW480 cells in the xenograft model was suppressed by co-planting NBR2-overexpressed macrophages and was facilitated by the co-planting of NBR2-knockdown macrophages. The release of inflammatory factors was induced, M1 polarization was facilitated, and M2 polarization was suppressed in tumor tissues in the NBR2-overexpressed group, which were all reversed in the NBR2-knockdown group. Therefore, the lncRNA NBR2 suppressed the progression of colorectal cancer in vitro and in vivo by regulating TAM polarization.Fuji LaiHuiqin ZhangBinbin XuYangyang XieHua YuTaylor & Francis Grouparticlelncrna nbr 2colorectal cancertumor associated macrophagepolarizationBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 5462-5475 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lncrna nbr 2 colorectal cancer tumor associated macrophage polarization Biotechnology TP248.13-248.65
spellingShingle	lncrna nbr 2 colorectal cancer tumor associated macrophage polarization Biotechnology TP248.13-248.65 Fuji Lai Huiqin Zhang Binbin Xu Yangyang Xie Hua Yu Long non-coding RNA NBR2 suppresses the progress of colorectal cancer in vitro and in vivo by regulating the polarization of TAM
description	Colorectal cancer (CRC) threatens the health of patients with high mortality, which lacks sensitive biomarkers for diagnosis to improve total survival. The lncRNA NBR2 is reported to be downregulated in CRC and suppresses the proliferation of CRC cells. However, the underlying mechanisms remain unclear. The present study aimed to explore the regulatory function of the lncRNA NBR2 on tumor-associated macrophage (TAM) polarization and its consequent anti-tumor effect. Two CRC cell lines were used in this study. We found that the lncRNA NBR2, TNF-α, and HLA-DR were downregulated, and Arg-1, CD163, CD206, and IL-4 were upregulated in CRC tumors. M1 polarization was activated and M2 polarization was suppressed in NBR2-overexpressed macrophages, accompanied by increased production of inflammatory factors, decreased proliferation, and inhibited migration ability in the co-culture system of HCT-116 cells (SW480 cells) and NBR2-overexpressed macrophages. The promoted proliferation and migration were observed in the co-culture system of HCT-116 cells (SW480 cells) and NBR2-knockdown macrophages. The tumor growth of both HCT-116 cells and SW480 cells in the xenograft model was suppressed by co-planting NBR2-overexpressed macrophages and was facilitated by the co-planting of NBR2-knockdown macrophages. The release of inflammatory factors was induced, M1 polarization was facilitated, and M2 polarization was suppressed in tumor tissues in the NBR2-overexpressed group, which were all reversed in the NBR2-knockdown group. Therefore, the lncRNA NBR2 suppressed the progression of colorectal cancer in vitro and in vivo by regulating TAM polarization.
format	article
author	Fuji Lai Huiqin Zhang Binbin Xu Yangyang Xie Hua Yu
author_facet	Fuji Lai Huiqin Zhang Binbin Xu Yangyang Xie Hua Yu
author_sort	Fuji Lai
title	Long non-coding RNA NBR2 suppresses the progress of colorectal cancer in vitro and in vivo by regulating the polarization of TAM
title_short	Long non-coding RNA NBR2 suppresses the progress of colorectal cancer in vitro and in vivo by regulating the polarization of TAM
title_full	Long non-coding RNA NBR2 suppresses the progress of colorectal cancer in vitro and in vivo by regulating the polarization of TAM
title_fullStr	Long non-coding RNA NBR2 suppresses the progress of colorectal cancer in vitro and in vivo by regulating the polarization of TAM
title_full_unstemmed	Long non-coding RNA NBR2 suppresses the progress of colorectal cancer in vitro and in vivo by regulating the polarization of TAM
title_sort	long non-coding rna nbr2 suppresses the progress of colorectal cancer in vitro and in vivo by regulating the polarization of tam
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/999f84986c3e4be89b81ff21b95a7c5f
work_keys_str_mv	AT fujilai longnoncodingrnanbr2suppressestheprogressofcolorectalcancerinvitroandinvivobyregulatingthepolarizationoftam AT huiqinzhang longnoncodingrnanbr2suppressestheprogressofcolorectalcancerinvitroandinvivobyregulatingthepolarizationoftam AT binbinxu longnoncodingrnanbr2suppressestheprogressofcolorectalcancerinvitroandinvivobyregulatingthepolarizationoftam AT yangyangxie longnoncodingrnanbr2suppressestheprogressofcolorectalcancerinvitroandinvivobyregulatingthepolarizationoftam AT huayu longnoncodingrnanbr2suppressestheprogressofcolorectalcancerinvitroandinvivobyregulatingthepolarizationoftam
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Long non-coding RNA NBR2 suppresses the progress of colorectal cancer in vitro and in vivo by regulating the polarization of TAM

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