Deciphering the Molecular Basis for Attenuation of <i>Flavobacterium columnare</i> Strain Fc1723 Used as Modified Live Vaccine against Columnaris Disease

Vaccines are widely employed in aquaculture to prevent bacterial infections, but their use by the U.S. catfish industry is very limited. One of the main diseases affecting catfish aquaculture is columnaris disease, caused by the bacterial pathogen <i>Flavobacterium columnare</i>. In 2011...

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Autores principales:	Wenlong Cai, Covadonga R. Arias
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Flavobacterium columnare</i> columnaris disease attenuated vaccine rifampin catfish Medicine R
Acceso en línea:	https://doaj.org/article/99a1b2ec02a1483b8c10d357809917e9
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spelling	oai:doaj.org-article:99a1b2ec02a1483b8c10d357809917e92021-11-25T19:11:42ZDeciphering the Molecular Basis for Attenuation of <i>Flavobacterium columnare</i> Strain Fc1723 Used as Modified Live Vaccine against Columnaris Disease10.3390/vaccines91113702076-393Xhttps://doaj.org/article/99a1b2ec02a1483b8c10d357809917e92021-11-01T00:00:00Zhttps://www.mdpi.com/2076-393X/9/11/1370https://doaj.org/toc/2076-393XVaccines are widely employed in aquaculture to prevent bacterial infections, but their use by the U.S. catfish industry is very limited. One of the main diseases affecting catfish aquaculture is columnaris disease, caused by the bacterial pathogen <i>Flavobacterium columnare</i>. In 2011, a modified-live vaccine against columnaris disease was developed by selecting mutants that were resistant to rifampin. The previous study has suggested that this vaccine is stable, safe, and effective, but the mechanisms that resulted in attenuation remained uncharacterized. To understand the molecular basis for attenuation, a comparative genomic analysis was conducted to identify specific point mutations. The PacBio RS long-read sequencing platform was used to obtain draft genomes of the mutant attenuated strain (Fc1723) and the parent virulent strain (FcB27). Sequence-based genome comparison identified 16 single nucleotide polymorphisms (SNP) unique to the mutant. Genes that contained mutations were involved in rifampin resistance, gliding motility, DNA transcription, toxin secretion, and extracellular protease synthesis. The results also found that the vaccine strain formed biofilm at a significantly lower rate than the parent strain. These observations suggested that the rifampin-resistant phenotype and the associated attenuation of the vaccine strain result from the altered activity of RNA polymerase (RpoB) and possible disrupted protein secretion systems.Wenlong CaiCovadonga R. AriasMDPI AGarticle<i>Flavobacterium columnare</i>columnaris diseaseattenuated vaccinerifampincatfishMedicineRENVaccines, Vol 9, Iss 1370, p 1370 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Flavobacterium columnare</i> columnaris disease attenuated vaccine rifampin catfish Medicine R
spellingShingle	<i>Flavobacterium columnare</i> columnaris disease attenuated vaccine rifampin catfish Medicine R Wenlong Cai Covadonga R. Arias Deciphering the Molecular Basis for Attenuation of <i>Flavobacterium columnare</i> Strain Fc1723 Used as Modified Live Vaccine against Columnaris Disease
description	Vaccines are widely employed in aquaculture to prevent bacterial infections, but their use by the U.S. catfish industry is very limited. One of the main diseases affecting catfish aquaculture is columnaris disease, caused by the bacterial pathogen <i>Flavobacterium columnare</i>. In 2011, a modified-live vaccine against columnaris disease was developed by selecting mutants that were resistant to rifampin. The previous study has suggested that this vaccine is stable, safe, and effective, but the mechanisms that resulted in attenuation remained uncharacterized. To understand the molecular basis for attenuation, a comparative genomic analysis was conducted to identify specific point mutations. The PacBio RS long-read sequencing platform was used to obtain draft genomes of the mutant attenuated strain (Fc1723) and the parent virulent strain (FcB27). Sequence-based genome comparison identified 16 single nucleotide polymorphisms (SNP) unique to the mutant. Genes that contained mutations were involved in rifampin resistance, gliding motility, DNA transcription, toxin secretion, and extracellular protease synthesis. The results also found that the vaccine strain formed biofilm at a significantly lower rate than the parent strain. These observations suggested that the rifampin-resistant phenotype and the associated attenuation of the vaccine strain result from the altered activity of RNA polymerase (RpoB) and possible disrupted protein secretion systems.
format	article
author	Wenlong Cai Covadonga R. Arias
author_facet	Wenlong Cai Covadonga R. Arias
author_sort	Wenlong Cai
title	Deciphering the Molecular Basis for Attenuation of <i>Flavobacterium columnare</i> Strain Fc1723 Used as Modified Live Vaccine against Columnaris Disease
title_short	Deciphering the Molecular Basis for Attenuation of <i>Flavobacterium columnare</i> Strain Fc1723 Used as Modified Live Vaccine against Columnaris Disease
title_full	Deciphering the Molecular Basis for Attenuation of <i>Flavobacterium columnare</i> Strain Fc1723 Used as Modified Live Vaccine against Columnaris Disease
title_fullStr	Deciphering the Molecular Basis for Attenuation of <i>Flavobacterium columnare</i> Strain Fc1723 Used as Modified Live Vaccine against Columnaris Disease
title_full_unstemmed	Deciphering the Molecular Basis for Attenuation of <i>Flavobacterium columnare</i> Strain Fc1723 Used as Modified Live Vaccine against Columnaris Disease
title_sort	deciphering the molecular basis for attenuation of <i>flavobacterium columnare</i> strain fc1723 used as modified live vaccine against columnaris disease
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/99a1b2ec02a1483b8c10d357809917e9
work_keys_str_mv	AT wenlongcai decipheringthemolecularbasisforattenuationofiflavobacteriumcolumnareistrainfc1723usedasmodifiedlivevaccineagainstcolumnarisdisease AT covadongararias decipheringthemolecularbasisforattenuationofiflavobacteriumcolumnareistrainfc1723usedasmodifiedlivevaccineagainstcolumnarisdisease
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Deciphering the Molecular Basis for Attenuation of <i>Flavobacterium columnare</i> Strain Fc1723 Used as Modified Live Vaccine against Columnaris Disease

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