Reliability and variation in mitochondrial respiration in human adipose tissue

Adipose tissue mitochondrial function is gaining increasing interest since it is a good marker of overall health. Methodological challenges and variability in assessing mitochondrial respiration in fresh adipose tissue with high-resolution respirometry are unknown and should be explored. Mitochondri...

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Autores principales:	Ronni Eg Sahl, Eva Frederikke Høy Helms, Malte Schmücker, Mathias Flensted-Jensen, Arthur Ingersen, Thomas Morville, Flemming Dela, Jørn Wulff Helge, Steen Larsen
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	adipose tissue human mitochondria high resolution respirometry coefficient of variation Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/99a290dd0b034b6082c2a88f46948afa
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spelling	oai:doaj.org-article:99a290dd0b034b6082c2a88f46948afa2021-11-26T11:19:49ZReliability and variation in mitochondrial respiration in human adipose tissue2162-39452162-397X10.1080/21623945.2021.1991617https://doaj.org/article/99a290dd0b034b6082c2a88f46948afa2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21623945.2021.1991617https://doaj.org/toc/2162-3945https://doaj.org/toc/2162-397XAdipose tissue mitochondrial function is gaining increasing interest since it is a good marker of overall health. Methodological challenges and variability in assessing mitochondrial respiration in fresh adipose tissue with high-resolution respirometry are unknown and should be explored. Mitochondrial respiratory capacity (MRC) in human adipose tissue declines in a gradual manner when analyses are postponed 3 h and 24 h, with a statistically significant decline 24 h after obtaining the biopsy. This decline in MRC is associated with a reduced integrity of the outer mitochondrial membrane at both time points. This study suggests that the optimal amount of tissue to be used is 20 mg and that different technicians handling the biopsy do not affect MRC.Ronni Eg SahlEva Frederikke Høy HelmsMalte SchmückerMathias Flensted-JensenArthur IngersenThomas MorvilleFlemming DelaJørn Wulff HelgeSteen LarsenTaylor & Francis Grouparticleadipose tissuehumanmitochondriahigh resolution respirometrycoefficient of variationDiseases of the endocrine glands. Clinical endocrinologyRC648-665CytologyQH573-671PhysiologyQP1-981ENAdipocyte, Vol 10, Iss 1, Pp 605-611 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adipose tissue human mitochondria high resolution respirometry coefficient of variation Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981
spellingShingle	adipose tissue human mitochondria high resolution respirometry coefficient of variation Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981 Ronni Eg Sahl Eva Frederikke Høy Helms Malte Schmücker Mathias Flensted-Jensen Arthur Ingersen Thomas Morville Flemming Dela Jørn Wulff Helge Steen Larsen Reliability and variation in mitochondrial respiration in human adipose tissue
description	Adipose tissue mitochondrial function is gaining increasing interest since it is a good marker of overall health. Methodological challenges and variability in assessing mitochondrial respiration in fresh adipose tissue with high-resolution respirometry are unknown and should be explored. Mitochondrial respiratory capacity (MRC) in human adipose tissue declines in a gradual manner when analyses are postponed 3 h and 24 h, with a statistically significant decline 24 h after obtaining the biopsy. This decline in MRC is associated with a reduced integrity of the outer mitochondrial membrane at both time points. This study suggests that the optimal amount of tissue to be used is 20 mg and that different technicians handling the biopsy do not affect MRC.
format	article
author	Ronni Eg Sahl Eva Frederikke Høy Helms Malte Schmücker Mathias Flensted-Jensen Arthur Ingersen Thomas Morville Flemming Dela Jørn Wulff Helge Steen Larsen
author_facet	Ronni Eg Sahl Eva Frederikke Høy Helms Malte Schmücker Mathias Flensted-Jensen Arthur Ingersen Thomas Morville Flemming Dela Jørn Wulff Helge Steen Larsen
author_sort	Ronni Eg Sahl
title	Reliability and variation in mitochondrial respiration in human adipose tissue
title_short	Reliability and variation in mitochondrial respiration in human adipose tissue
title_full	Reliability and variation in mitochondrial respiration in human adipose tissue
title_fullStr	Reliability and variation in mitochondrial respiration in human adipose tissue
title_full_unstemmed	Reliability and variation in mitochondrial respiration in human adipose tissue
title_sort	reliability and variation in mitochondrial respiration in human adipose tissue
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/99a290dd0b034b6082c2a88f46948afa
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Reliability and variation in mitochondrial respiration in human adipose tissue

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