Short-term transcriptomic response to plasma membrane injury

Abstract Plasma membrane repair mechanisms are activated within seconds post-injury to promote rapid membrane resealing in eukaryotic cells and prevent cell death. However, less is known about the regeneration phase that follows and how cells respond to injury in the short-term. Here, we provide a g...

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Autores principales:	Swantje Christin Häger, Catarina Dias, Stine Lauritzen Sønder, André Vidas Olsen, Isabelle da Piedade, Anne Sofie Busk Heitmann, Elena Papaleo, Jesper Nylandsted
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/99adb3dc3d88401b94736e0dc7c6ae80
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spelling	oai:doaj.org-article:99adb3dc3d88401b94736e0dc7c6ae802021-12-02T19:16:47ZShort-term transcriptomic response to plasma membrane injury10.1038/s41598-021-98420-y2045-2322https://doaj.org/article/99adb3dc3d88401b94736e0dc7c6ae802021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98420-yhttps://doaj.org/toc/2045-2322Abstract Plasma membrane repair mechanisms are activated within seconds post-injury to promote rapid membrane resealing in eukaryotic cells and prevent cell death. However, less is known about the regeneration phase that follows and how cells respond to injury in the short-term. Here, we provide a genome-wide study into the mRNA expression profile of MCF-7 breast cancer cells exposed to injury by digitonin, a mild non-ionic detergent that permeabilizes the plasma membrane. We focused on the early transcriptional signature and found a time-dependent increase in the number of differentially expressed (> twofold, P < 0.05) genes (34, 114 and 236 genes at 20-, 40- and 60-min post-injury, respectively). Pathway analysis highlighted a robust and gradual three-part transcriptional response: (1) prompt activation of immediate-early response genes, (2) activation of specific MAPK cascades and (3) induction of inflammatory and immune pathways. Therefore, plasma membrane injury triggers a rapid and strong stress and immunogenic response. Our meta-analysis suggests that this is a conserved transcriptome response to plasma membrane injury across different cell and injury types. Taken together, our study shows that injury has profound effects on the transcriptome of wounded cells in the regeneration phase (subsequent to membrane resealing), which is likely to influence cellular status and has been previously overlooked.Swantje Christin HägerCatarina DiasStine Lauritzen SønderAndré Vidas OlsenIsabelle da PiedadeAnne Sofie Busk HeitmannElena PapaleoJesper NylandstedNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Swantje Christin Häger Catarina Dias Stine Lauritzen Sønder André Vidas Olsen Isabelle da Piedade Anne Sofie Busk Heitmann Elena Papaleo Jesper Nylandsted Short-term transcriptomic response to plasma membrane injury
description	Abstract Plasma membrane repair mechanisms are activated within seconds post-injury to promote rapid membrane resealing in eukaryotic cells and prevent cell death. However, less is known about the regeneration phase that follows and how cells respond to injury in the short-term. Here, we provide a genome-wide study into the mRNA expression profile of MCF-7 breast cancer cells exposed to injury by digitonin, a mild non-ionic detergent that permeabilizes the plasma membrane. We focused on the early transcriptional signature and found a time-dependent increase in the number of differentially expressed (> twofold, P < 0.05) genes (34, 114 and 236 genes at 20-, 40- and 60-min post-injury, respectively). Pathway analysis highlighted a robust and gradual three-part transcriptional response: (1) prompt activation of immediate-early response genes, (2) activation of specific MAPK cascades and (3) induction of inflammatory and immune pathways. Therefore, plasma membrane injury triggers a rapid and strong stress and immunogenic response. Our meta-analysis suggests that this is a conserved transcriptome response to plasma membrane injury across different cell and injury types. Taken together, our study shows that injury has profound effects on the transcriptome of wounded cells in the regeneration phase (subsequent to membrane resealing), which is likely to influence cellular status and has been previously overlooked.
format	article
author	Swantje Christin Häger Catarina Dias Stine Lauritzen Sønder André Vidas Olsen Isabelle da Piedade Anne Sofie Busk Heitmann Elena Papaleo Jesper Nylandsted
author_facet	Swantje Christin Häger Catarina Dias Stine Lauritzen Sønder André Vidas Olsen Isabelle da Piedade Anne Sofie Busk Heitmann Elena Papaleo Jesper Nylandsted
author_sort	Swantje Christin Häger
title	Short-term transcriptomic response to plasma membrane injury
title_short	Short-term transcriptomic response to plasma membrane injury
title_full	Short-term transcriptomic response to plasma membrane injury
title_fullStr	Short-term transcriptomic response to plasma membrane injury
title_full_unstemmed	Short-term transcriptomic response to plasma membrane injury
title_sort	short-term transcriptomic response to plasma membrane injury
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/99adb3dc3d88401b94736e0dc7c6ae80
work_keys_str_mv	AT swantjechristinhager shorttermtranscriptomicresponsetoplasmamembraneinjury AT catarinadias shorttermtranscriptomicresponsetoplasmamembraneinjury AT stinelauritzensønder shorttermtranscriptomicresponsetoplasmamembraneinjury AT andrevidasolsen shorttermtranscriptomicresponsetoplasmamembraneinjury AT isabelledapiedade shorttermtranscriptomicresponsetoplasmamembraneinjury AT annesofiebuskheitmann shorttermtranscriptomicresponsetoplasmamembraneinjury AT elenapapaleo shorttermtranscriptomicresponsetoplasmamembraneinjury AT jespernylandsted shorttermtranscriptomicresponsetoplasmamembraneinjury
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Short-term transcriptomic response to plasma membrane injury

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