Leucosceptoside A from Devil’s Claw Modulates Psoriasis-like Inflammation via Suppression of the PI3K/AKT Signaling Pathway in Keratinocytes

Psoriasis is a chronic inflammatory skin condition characterized by abnormal keratinocyte proliferation and differentiation that is accompanied with dysregulated immune response and abnormal vascularization. Devil’s claw (<i>Harpagophytum procumbens</i> (Burch.) DC. ex Meisn.) tubers ext...

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Autores principales:	Ivanka K. Koycheva, Liliya V. Mihaylova, Monika N. Todorova, Zhivka P. Balcheva-Sivenova, Kalina Alipieva, Claudio Ferrante, Giustino Orlando, Milen I. Georgiev
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	psoriasis keratinocytes inflammation verbascoside leucosceptoside A devil’s claw Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/99b65710ec314300a165d3fbc4a59782
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spelling	oai:doaj.org-article:99b65710ec314300a165d3fbc4a597822021-11-25T18:29:11ZLeucosceptoside A from Devil’s Claw Modulates Psoriasis-like Inflammation via Suppression of the PI3K/AKT Signaling Pathway in Keratinocytes10.3390/molecules262270141420-3049https://doaj.org/article/99b65710ec314300a165d3fbc4a597822021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/7014https://doaj.org/toc/1420-3049Psoriasis is a chronic inflammatory skin condition characterized by abnormal keratinocyte proliferation and differentiation that is accompanied with dysregulated immune response and abnormal vascularization. Devil’s claw (<i>Harpagophytum procumbens</i> (Burch.) DC. ex Meisn.) tubers extract has been used both systemically and topically for treatment of chronic inflammatory diseases such as arthritis, osteoporosis, inflammatory bowel disease, among others. However, its potential mechanisms of action against psoriasis remains poorly investigated. The human keratinocyte HaCaT cell line is a well-accepted in vitro model system for inflammatory skin disorders such as psoriasis. The present study involved an exploration of the effect of biotechnologically produced <i>H. procumbens</i> (HP) cell suspension extract and pure phenylethanoid glycosides verbascoside (VER) and leucosceptoside A (LEU) in interferon (IFN)-γ/interleukin (IL)-17A/IL-22-stimulated HaCaT cells as a model of psoriasis-like inflammation. Changes in key inflammatory signaling pathways related to psoriasis development were detected by reverse transcription polymerase chain reaction and western blotting. Treatment with LEU, but not VER and HP extract improved psoriasis-related inflammation via suppression of the PI3K/AKT signaling in IFN-γ/IL-17A/IL-22-stimulated HaCaT cells. Our results suggest that LEU may exhibit therapeutic potential against psoriasis by regulating keratinocyte differentiation through inhibition of the PI3K/AKT pathway.Ivanka K. KoychevaLiliya V. MihaylovaMonika N. TodorovaZhivka P. Balcheva-SivenovaKalina AlipievaClaudio FerranteGiustino OrlandoMilen I. GeorgievMDPI AGarticlepsoriasiskeratinocytesinflammationverbascosideleucosceptoside Adevil’s clawOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 7014, p 7014 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	psoriasis keratinocytes inflammation verbascoside leucosceptoside A devil’s claw Organic chemistry QD241-441
spellingShingle	psoriasis keratinocytes inflammation verbascoside leucosceptoside A devil’s claw Organic chemistry QD241-441 Ivanka K. Koycheva Liliya V. Mihaylova Monika N. Todorova Zhivka P. Balcheva-Sivenova Kalina Alipieva Claudio Ferrante Giustino Orlando Milen I. Georgiev Leucosceptoside A from Devil’s Claw Modulates Psoriasis-like Inflammation via Suppression of the PI3K/AKT Signaling Pathway in Keratinocytes
description	Psoriasis is a chronic inflammatory skin condition characterized by abnormal keratinocyte proliferation and differentiation that is accompanied with dysregulated immune response and abnormal vascularization. Devil’s claw (<i>Harpagophytum procumbens</i> (Burch.) DC. ex Meisn.) tubers extract has been used both systemically and topically for treatment of chronic inflammatory diseases such as arthritis, osteoporosis, inflammatory bowel disease, among others. However, its potential mechanisms of action against psoriasis remains poorly investigated. The human keratinocyte HaCaT cell line is a well-accepted in vitro model system for inflammatory skin disorders such as psoriasis. The present study involved an exploration of the effect of biotechnologically produced <i>H. procumbens</i> (HP) cell suspension extract and pure phenylethanoid glycosides verbascoside (VER) and leucosceptoside A (LEU) in interferon (IFN)-γ/interleukin (IL)-17A/IL-22-stimulated HaCaT cells as a model of psoriasis-like inflammation. Changes in key inflammatory signaling pathways related to psoriasis development were detected by reverse transcription polymerase chain reaction and western blotting. Treatment with LEU, but not VER and HP extract improved psoriasis-related inflammation via suppression of the PI3K/AKT signaling in IFN-γ/IL-17A/IL-22-stimulated HaCaT cells. Our results suggest that LEU may exhibit therapeutic potential against psoriasis by regulating keratinocyte differentiation through inhibition of the PI3K/AKT pathway.
format	article
author	Ivanka K. Koycheva Liliya V. Mihaylova Monika N. Todorova Zhivka P. Balcheva-Sivenova Kalina Alipieva Claudio Ferrante Giustino Orlando Milen I. Georgiev
author_facet	Ivanka K. Koycheva Liliya V. Mihaylova Monika N. Todorova Zhivka P. Balcheva-Sivenova Kalina Alipieva Claudio Ferrante Giustino Orlando Milen I. Georgiev
author_sort	Ivanka K. Koycheva
title	Leucosceptoside A from Devil’s Claw Modulates Psoriasis-like Inflammation via Suppression of the PI3K/AKT Signaling Pathway in Keratinocytes
title_short	Leucosceptoside A from Devil’s Claw Modulates Psoriasis-like Inflammation via Suppression of the PI3K/AKT Signaling Pathway in Keratinocytes
title_full	Leucosceptoside A from Devil’s Claw Modulates Psoriasis-like Inflammation via Suppression of the PI3K/AKT Signaling Pathway in Keratinocytes
title_fullStr	Leucosceptoside A from Devil’s Claw Modulates Psoriasis-like Inflammation via Suppression of the PI3K/AKT Signaling Pathway in Keratinocytes
title_full_unstemmed	Leucosceptoside A from Devil’s Claw Modulates Psoriasis-like Inflammation via Suppression of the PI3K/AKT Signaling Pathway in Keratinocytes
title_sort	leucosceptoside a from devil’s claw modulates psoriasis-like inflammation via suppression of the pi3k/akt signaling pathway in keratinocytes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/99b65710ec314300a165d3fbc4a59782
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Leucosceptoside A from Devil’s Claw Modulates Psoriasis-like Inflammation via Suppression of the PI3K/AKT Signaling Pathway in Keratinocytes

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