Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation

CRISPR-Cas9 is a powerful technique for manipulating the human genome, however temporal and spatial control of activity would facilitate safer, more selective use. Here the authors incorporate aptazymes into guide RNAs to allow small molecule activation of CRISPR-Cas9.

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Detalles Bibliográficos
Autores principales:	Weixin Tang, Johnny H. Hu, David R. Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/99b6d665d5524fc89acbd0b21587a335
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spelling	oai:doaj.org-article:99b6d665d5524fc89acbd0b21587a3352021-12-02T17:01:19ZAptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation10.1038/ncomms159392041-1723https://doaj.org/article/99b6d665d5524fc89acbd0b21587a3352017-06-01T00:00:00Zhttps://doi.org/10.1038/ncomms15939https://doaj.org/toc/2041-1723CRISPR-Cas9 is a powerful technique for manipulating the human genome, however temporal and spatial control of activity would facilitate safer, more selective use. Here the authors incorporate aptazymes into guide RNAs to allow small molecule activation of CRISPR-Cas9.Weixin TangJohnny H. HuDavid R. LiuNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Weixin Tang Johnny H. Hu David R. Liu Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
description	CRISPR-Cas9 is a powerful technique for manipulating the human genome, however temporal and spatial control of activity would facilitate safer, more selective use. Here the authors incorporate aptazymes into guide RNAs to allow small molecule activation of CRISPR-Cas9.
format	article
author	Weixin Tang Johnny H. Hu David R. Liu
author_facet	Weixin Tang Johnny H. Hu David R. Liu
author_sort	Weixin Tang
title	Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
title_short	Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
title_full	Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
title_fullStr	Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
title_full_unstemmed	Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
title_sort	aptazyme-embedded guide rnas enable ligand-responsive genome editing and transcriptional activation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/99b6d665d5524fc89acbd0b21587a335
work_keys_str_mv	AT weixintang aptazymeembeddedguidernasenableligandresponsivegenomeeditingandtranscriptionalactivation AT johnnyhhu aptazymeembeddedguidernasenableligandresponsivegenomeeditingandtranscriptionalactivation AT davidrliu aptazymeembeddedguidernasenableligandresponsivegenomeeditingandtranscriptionalactivation
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Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation

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