VAPA, an innovative "virus-acquisition phenotyping assay" opens new horizons in research into the vector-transmission of plant viruses.

Host-to-host transmission--a key step in plant virus infection cycles--is ensured predominantly by vectors, especially aphids and related insects. A deeper understanding of the mechanisms of virus acquisition, which is critical to vector-transmission, might help to design future virus control strate...

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Autores principales:	Alexandre Martinière, Jean-Luc Macia, Guillaume Bagnolini, Chiraz Jridi, Aurélie Bak, Stéphane Blanc, Martin Drucker
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/99ba8938b4774e72a22e4d5210c7ecb0
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spelling	oai:doaj.org-article:99ba8938b4774e72a22e4d5210c7ecb02021-11-18T06:48:19ZVAPA, an innovative "virus-acquisition phenotyping assay" opens new horizons in research into the vector-transmission of plant viruses.1932-620310.1371/journal.pone.0023241https://doaj.org/article/99ba8938b4774e72a22e4d5210c7ecb02011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21853093/?tool=EBIhttps://doaj.org/toc/1932-6203Host-to-host transmission--a key step in plant virus infection cycles--is ensured predominantly by vectors, especially aphids and related insects. A deeper understanding of the mechanisms of virus acquisition, which is critical to vector-transmission, might help to design future virus control strategies, because any newly discovered molecular or cellular process is a potential target for hampering viral spread within host populations. With this aim in mind, an aphid membrane-feeding assay was developed where aphids transmitted two non-circulative viruses [cauliflower mosaic virus (CaMV) and turnip mosaic virus] from infected protoplasts. In this assay, virus acquisition occurs exclusively from living cells. Most interestingly, we also show that CaMV is less efficiently transmitted by aphids in the presence of oryzalin--a microtubule-depolymerising drug. The example presented here demonstrates that our technically simple "virus-acquisition phenotyping assay" (VAPA) provides a first opportunity to implement correlative studies relating the physiological state of infected plant cells to vector-transmission efficiency.Alexandre MartinièreJean-Luc MaciaGuillaume BagnoliniChiraz JridiAurélie BakStéphane BlancMartin DruckerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e23241 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexandre Martinière Jean-Luc Macia Guillaume Bagnolini Chiraz Jridi Aurélie Bak Stéphane Blanc Martin Drucker VAPA, an innovative "virus-acquisition phenotyping assay" opens new horizons in research into the vector-transmission of plant viruses.
description	Host-to-host transmission--a key step in plant virus infection cycles--is ensured predominantly by vectors, especially aphids and related insects. A deeper understanding of the mechanisms of virus acquisition, which is critical to vector-transmission, might help to design future virus control strategies, because any newly discovered molecular or cellular process is a potential target for hampering viral spread within host populations. With this aim in mind, an aphid membrane-feeding assay was developed where aphids transmitted two non-circulative viruses [cauliflower mosaic virus (CaMV) and turnip mosaic virus] from infected protoplasts. In this assay, virus acquisition occurs exclusively from living cells. Most interestingly, we also show that CaMV is less efficiently transmitted by aphids in the presence of oryzalin--a microtubule-depolymerising drug. The example presented here demonstrates that our technically simple "virus-acquisition phenotyping assay" (VAPA) provides a first opportunity to implement correlative studies relating the physiological state of infected plant cells to vector-transmission efficiency.
format	article
author	Alexandre Martinière Jean-Luc Macia Guillaume Bagnolini Chiraz Jridi Aurélie Bak Stéphane Blanc Martin Drucker
author_facet	Alexandre Martinière Jean-Luc Macia Guillaume Bagnolini Chiraz Jridi Aurélie Bak Stéphane Blanc Martin Drucker
author_sort	Alexandre Martinière
title	VAPA, an innovative "virus-acquisition phenotyping assay" opens new horizons in research into the vector-transmission of plant viruses.
title_short	VAPA, an innovative "virus-acquisition phenotyping assay" opens new horizons in research into the vector-transmission of plant viruses.
title_full	VAPA, an innovative "virus-acquisition phenotyping assay" opens new horizons in research into the vector-transmission of plant viruses.
title_fullStr	VAPA, an innovative "virus-acquisition phenotyping assay" opens new horizons in research into the vector-transmission of plant viruses.
title_full_unstemmed	VAPA, an innovative "virus-acquisition phenotyping assay" opens new horizons in research into the vector-transmission of plant viruses.
title_sort	vapa, an innovative "virus-acquisition phenotyping assay" opens new horizons in research into the vector-transmission of plant viruses.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/99ba8938b4774e72a22e4d5210c7ecb0
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VAPA, an innovative "virus-acquisition phenotyping assay" opens new horizons in research into the vector-transmission of plant viruses.

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