Cryptococcal cell morphology affects host cell interactions and pathogenicity.
Cryptococcus neoformans is a common life-threatening human fungal pathogen. The size of cryptococcal cells is typically 5 to 10 microm. Cell enlargement was observed in vivo, producing cells up to 100 microm. These morphological changes in cell size affected pathogenicity via reducing phagocytosis b...
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Public Library of Science (PLoS)
2010
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oai:doaj.org-article:99bcbef7a4be44a8b9223effd38d94742021-12-02T20:00:34ZCryptococcal cell morphology affects host cell interactions and pathogenicity.1553-73661553-737410.1371/journal.ppat.1000953https://doaj.org/article/99bcbef7a4be44a8b9223effd38d94742010-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20585559/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Cryptococcus neoformans is a common life-threatening human fungal pathogen. The size of cryptococcal cells is typically 5 to 10 microm. Cell enlargement was observed in vivo, producing cells up to 100 microm. These morphological changes in cell size affected pathogenicity via reducing phagocytosis by host mononuclear cells, increasing resistance to oxidative and nitrosative stress, and correlated with reduced penetration of the central nervous system. Cell enlargement was stimulated by coinfection with strains of opposite mating type, and ste3aDelta pheromone receptor mutant strains had reduced cell enlargement. Finally, analysis of DNA content in this novel cell type revealed that these enlarged cells were polyploid, uninucleate, and produced daughter cells in vivo. These results describe a novel mechanism by which C. neoformans evades host phagocytosis to allow survival of a subset of the population at early stages of infection. Thus, morphological changes play unique and specialized roles during infection.Laura H OkagakiAnna K StrainJudith N NielsenCaroline CharlierNicholas J BaltesFabrice ChrétienJoseph HeitmanFrançoise DromerKirsten NielsenPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 6, Iss 6, p e1000953 (2010) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 |
| spellingShingle |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Laura H Okagaki Anna K Strain Judith N Nielsen Caroline Charlier Nicholas J Baltes Fabrice Chrétien Joseph Heitman Françoise Dromer Kirsten Nielsen Cryptococcal cell morphology affects host cell interactions and pathogenicity. |
| description |
Cryptococcus neoformans is a common life-threatening human fungal pathogen. The size of cryptococcal cells is typically 5 to 10 microm. Cell enlargement was observed in vivo, producing cells up to 100 microm. These morphological changes in cell size affected pathogenicity via reducing phagocytosis by host mononuclear cells, increasing resistance to oxidative and nitrosative stress, and correlated with reduced penetration of the central nervous system. Cell enlargement was stimulated by coinfection with strains of opposite mating type, and ste3aDelta pheromone receptor mutant strains had reduced cell enlargement. Finally, analysis of DNA content in this novel cell type revealed that these enlarged cells were polyploid, uninucleate, and produced daughter cells in vivo. These results describe a novel mechanism by which C. neoformans evades host phagocytosis to allow survival of a subset of the population at early stages of infection. Thus, morphological changes play unique and specialized roles during infection. |
| format |
article |
| author |
Laura H Okagaki Anna K Strain Judith N Nielsen Caroline Charlier Nicholas J Baltes Fabrice Chrétien Joseph Heitman Françoise Dromer Kirsten Nielsen |
| author_facet |
Laura H Okagaki Anna K Strain Judith N Nielsen Caroline Charlier Nicholas J Baltes Fabrice Chrétien Joseph Heitman Françoise Dromer Kirsten Nielsen |
| author_sort |
Laura H Okagaki |
| title |
Cryptococcal cell morphology affects host cell interactions and pathogenicity. |
| title_short |
Cryptococcal cell morphology affects host cell interactions and pathogenicity. |
| title_full |
Cryptococcal cell morphology affects host cell interactions and pathogenicity. |
| title_fullStr |
Cryptococcal cell morphology affects host cell interactions and pathogenicity. |
| title_full_unstemmed |
Cryptococcal cell morphology affects host cell interactions and pathogenicity. |
| title_sort |
cryptococcal cell morphology affects host cell interactions and pathogenicity. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2010 |
| url |
https://doaj.org/article/99bcbef7a4be44a8b9223effd38d9474 |
| work_keys_str_mv |
AT laurahokagaki cryptococcalcellmorphologyaffectshostcellinteractionsandpathogenicity AT annakstrain cryptococcalcellmorphologyaffectshostcellinteractionsandpathogenicity AT judithnnielsen cryptococcalcellmorphologyaffectshostcellinteractionsandpathogenicity AT carolinecharlier cryptococcalcellmorphologyaffectshostcellinteractionsandpathogenicity AT nicholasjbaltes cryptococcalcellmorphologyaffectshostcellinteractionsandpathogenicity AT fabricechretien cryptococcalcellmorphologyaffectshostcellinteractionsandpathogenicity AT josephheitman cryptococcalcellmorphologyaffectshostcellinteractionsandpathogenicity AT francoisedromer cryptococcalcellmorphologyaffectshostcellinteractionsandpathogenicity AT kirstennielsen cryptococcalcellmorphologyaffectshostcellinteractionsandpathogenicity |
| _version_ |
1718375739111243776 |