Nanoplasmonic Upconverting Nanoparticles as Orientation Sensors for Single Particle Microscopy
Abstract We showed that the anisotropic disk shape of nanoplasmonic upconverting nanoparticles (NP-UCNPs) creates changes in fluorescence intensity during rotational motion. We determined the orientation by a three-fold change in fluorescence intensity. We further found that the luminescence intensi...
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| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2017
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/99c7a62c1e8941529cc750523a4b196f |
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| Sumario: | Abstract We showed that the anisotropic disk shape of nanoplasmonic upconverting nanoparticles (NP-UCNPs) creates changes in fluorescence intensity during rotational motion. We determined the orientation by a three-fold change in fluorescence intensity. We further found that the luminescence intensity was strongly dependent on the particle orientation and on polarization of the excitation light. The luminescence intensity showed a three-fold difference between flat and on-edge orientations. The intensity also varied sinusoidally with the polarization of the incident light, with an Imax/Imin ratio of up to 2.02. Both the orientation dependence and Imax/Imin are dependent on the presence of a gold shell on the UCNP. Because the fluorescence depends on the NP’s orientation, the rotational motion of biomolecules coupled to the NP can be detected. Finally, we tracked the real-time rotational motion of a single NP-UCNP in solution between slide and coverslip with diffusivity up to 10−2 μm2s−1. |
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