Nanoplasmonic Upconverting Nanoparticles as Orientation Sensors for Single Particle Microscopy

Abstract We showed that the anisotropic disk shape of nanoplasmonic upconverting nanoparticles (NP-UCNPs) creates changes in fluorescence intensity during rotational motion. We determined the orientation by a three-fold change in fluorescence intensity. We further found that the luminescence intensi...

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Autores principales:	Kory K. Green, Janina Wirth, Shuang F. Lim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/99c7a62c1e8941529cc750523a4b196f
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spelling	oai:doaj.org-article:99c7a62c1e8941529cc750523a4b196f2021-12-02T11:40:51ZNanoplasmonic Upconverting Nanoparticles as Orientation Sensors for Single Particle Microscopy10.1038/s41598-017-00869-32045-2322https://doaj.org/article/99c7a62c1e8941529cc750523a4b196f2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00869-3https://doaj.org/toc/2045-2322Abstract We showed that the anisotropic disk shape of nanoplasmonic upconverting nanoparticles (NP-UCNPs) creates changes in fluorescence intensity during rotational motion. We determined the orientation by a three-fold change in fluorescence intensity. We further found that the luminescence intensity was strongly dependent on the particle orientation and on polarization of the excitation light. The luminescence intensity showed a three-fold difference between flat and on-edge orientations. The intensity also varied sinusoidally with the polarization of the incident light, with an Imax/Imin ratio of up to 2.02. Both the orientation dependence and Imax/Imin are dependent on the presence of a gold shell on the UCNP. Because the fluorescence depends on the NP’s orientation, the rotational motion of biomolecules coupled to the NP can be detected. Finally, we tracked the real-time rotational motion of a single NP-UCNP in solution between slide and coverslip with diffusivity up to 10−2 μm2s−1.Kory K. GreenJanina WirthShuang F. LimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kory K. Green Janina Wirth Shuang F. Lim Nanoplasmonic Upconverting Nanoparticles as Orientation Sensors for Single Particle Microscopy
description	Abstract We showed that the anisotropic disk shape of nanoplasmonic upconverting nanoparticles (NP-UCNPs) creates changes in fluorescence intensity during rotational motion. We determined the orientation by a three-fold change in fluorescence intensity. We further found that the luminescence intensity was strongly dependent on the particle orientation and on polarization of the excitation light. The luminescence intensity showed a three-fold difference between flat and on-edge orientations. The intensity also varied sinusoidally with the polarization of the incident light, with an Imax/Imin ratio of up to 2.02. Both the orientation dependence and Imax/Imin are dependent on the presence of a gold shell on the UCNP. Because the fluorescence depends on the NP’s orientation, the rotational motion of biomolecules coupled to the NP can be detected. Finally, we tracked the real-time rotational motion of a single NP-UCNP in solution between slide and coverslip with diffusivity up to 10−2 μm2s−1.
format	article
author	Kory K. Green Janina Wirth Shuang F. Lim
author_facet	Kory K. Green Janina Wirth Shuang F. Lim
author_sort	Kory K. Green
title	Nanoplasmonic Upconverting Nanoparticles as Orientation Sensors for Single Particle Microscopy
title_short	Nanoplasmonic Upconverting Nanoparticles as Orientation Sensors for Single Particle Microscopy
title_full	Nanoplasmonic Upconverting Nanoparticles as Orientation Sensors for Single Particle Microscopy
title_fullStr	Nanoplasmonic Upconverting Nanoparticles as Orientation Sensors for Single Particle Microscopy
title_full_unstemmed	Nanoplasmonic Upconverting Nanoparticles as Orientation Sensors for Single Particle Microscopy
title_sort	nanoplasmonic upconverting nanoparticles as orientation sensors for single particle microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/99c7a62c1e8941529cc750523a4b196f
work_keys_str_mv	AT korykgreen nanoplasmonicupconvertingnanoparticlesasorientationsensorsforsingleparticlemicroscopy AT janinawirth nanoplasmonicupconvertingnanoparticlesasorientationsensorsforsingleparticlemicroscopy AT shuangflim nanoplasmonicupconvertingnanoparticlesasorientationsensorsforsingleparticlemicroscopy
_version_	1718395532504727552

Nanoplasmonic Upconverting Nanoparticles as Orientation Sensors for Single Particle Microscopy

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