OralDisk: A Chair-Side Compatible Molecular Platform Using Whole Saliva for Monitoring Oral Health at the Dental Practice
Periodontitis and dental caries are two major bacterially induced, non-communicable diseases that cause the deterioration of oral health, with implications in patients’ general health. Early, precise diagnosis and personalized monitoring are essential for the efficient prevention and management of t...
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MDPI AG
2021
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oai:doaj.org-article:99cf5461c1914ad484eb0d8614eb3dc22021-11-25T16:55:05ZOralDisk: A Chair-Side Compatible Molecular Platform Using Whole Saliva for Monitoring Oral Health at the Dental Practice10.3390/bios111104232079-6374https://doaj.org/article/99cf5461c1914ad484eb0d8614eb3dc22021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6374/11/11/423https://doaj.org/toc/2079-6374Periodontitis and dental caries are two major bacterially induced, non-communicable diseases that cause the deterioration of oral health, with implications in patients’ general health. Early, precise diagnosis and personalized monitoring are essential for the efficient prevention and management of these diseases. Here, we present a disk-shaped microfluidic platform (OralDisk) compatible with chair-side use that enables analysis of non-invasively collected whole saliva samples and molecular-based detection of ten bacteria: seven periodontitis-associated (<i>Aggregatibacter actinomycetemcomitans</i>, <i>Campylobacter rectus</i>, <i>Fusobacterium nucleatum</i>, <i>Prevotella intermedia</i>, <i>Porphyromonas gingivalis</i>, <i>Tannerella forsythia</i>, <i>Treponema denticola</i>) and three caries-associated (oral <i>Lactobacilli</i>, <i>Streptococcus mutans</i>, <i>Streptococcus sobrinus</i>). Each OralDisk test required 400 µL of homogenized whole saliva. The automated workflow included bacterial DNA extraction, purification and hydrolysis probe real-time PCR detection of the target pathogens. All reagents were pre-stored within the disk and sample-to-answer processing took < 3 h using a compact, customized processing device. A technical feasibility study (25 OralDisks) was conducted using samples from healthy, periodontitis and caries patients. The comparison of the OralDisk with a lab-based reference method revealed a ~90% agreement amongst targets detected as positive and negative. This shows the OralDisk’s potential and suitability for inclusion in larger prospective implementation studies in dental care settings.Desirée BaumgartnerBenita JohannsenMara SpechtJan LüddeckeMarkus RombachSebastian HinNils PaustFelix von StettenRoland ZengerleChristopher HerzJohannes R. PehamPune N. PaquéThomas AttinJoël S. JenzerPhilipp KörnerPatrick R. SchmidlinThomas ThurnheerFlorian J. WegehauptWendy E. KamanAndrew StubbsJohn P. HaysViorel RusuAlex MichieThomas BinslDavid StejskalMichal KarpíšekKai BaoNagihan BostanciGeorgios N. BelibasakisKonstantinos MitsakakisMDPI AGarticledental practicepoint-of-care diagnosticstreatment monitoringoral healthperiodontitiscariesBiotechnologyTP248.13-248.65ENBiosensors, Vol 11, Iss 423, p 423 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
dental practice point-of-care diagnostics treatment monitoring oral health periodontitis caries Biotechnology TP248.13-248.65 |
| spellingShingle |
dental practice point-of-care diagnostics treatment monitoring oral health periodontitis caries Biotechnology TP248.13-248.65 Desirée Baumgartner Benita Johannsen Mara Specht Jan Lüddecke Markus Rombach Sebastian Hin Nils Paust Felix von Stetten Roland Zengerle Christopher Herz Johannes R. Peham Pune N. Paqué Thomas Attin Joël S. Jenzer Philipp Körner Patrick R. Schmidlin Thomas Thurnheer Florian J. Wegehaupt Wendy E. Kaman Andrew Stubbs John P. Hays Viorel Rusu Alex Michie Thomas Binsl David Stejskal Michal Karpíšek Kai Bao Nagihan Bostanci Georgios N. Belibasakis Konstantinos Mitsakakis OralDisk: A Chair-Side Compatible Molecular Platform Using Whole Saliva for Monitoring Oral Health at the Dental Practice |
| description |
Periodontitis and dental caries are two major bacterially induced, non-communicable diseases that cause the deterioration of oral health, with implications in patients’ general health. Early, precise diagnosis and personalized monitoring are essential for the efficient prevention and management of these diseases. Here, we present a disk-shaped microfluidic platform (OralDisk) compatible with chair-side use that enables analysis of non-invasively collected whole saliva samples and molecular-based detection of ten bacteria: seven periodontitis-associated (<i>Aggregatibacter actinomycetemcomitans</i>, <i>Campylobacter rectus</i>, <i>Fusobacterium nucleatum</i>, <i>Prevotella intermedia</i>, <i>Porphyromonas gingivalis</i>, <i>Tannerella forsythia</i>, <i>Treponema denticola</i>) and three caries-associated (oral <i>Lactobacilli</i>, <i>Streptococcus mutans</i>, <i>Streptococcus sobrinus</i>). Each OralDisk test required 400 µL of homogenized whole saliva. The automated workflow included bacterial DNA extraction, purification and hydrolysis probe real-time PCR detection of the target pathogens. All reagents were pre-stored within the disk and sample-to-answer processing took < 3 h using a compact, customized processing device. A technical feasibility study (25 OralDisks) was conducted using samples from healthy, periodontitis and caries patients. The comparison of the OralDisk with a lab-based reference method revealed a ~90% agreement amongst targets detected as positive and negative. This shows the OralDisk’s potential and suitability for inclusion in larger prospective implementation studies in dental care settings. |
| format |
article |
| author |
Desirée Baumgartner Benita Johannsen Mara Specht Jan Lüddecke Markus Rombach Sebastian Hin Nils Paust Felix von Stetten Roland Zengerle Christopher Herz Johannes R. Peham Pune N. Paqué Thomas Attin Joël S. Jenzer Philipp Körner Patrick R. Schmidlin Thomas Thurnheer Florian J. Wegehaupt Wendy E. Kaman Andrew Stubbs John P. Hays Viorel Rusu Alex Michie Thomas Binsl David Stejskal Michal Karpíšek Kai Bao Nagihan Bostanci Georgios N. Belibasakis Konstantinos Mitsakakis |
| author_facet |
Desirée Baumgartner Benita Johannsen Mara Specht Jan Lüddecke Markus Rombach Sebastian Hin Nils Paust Felix von Stetten Roland Zengerle Christopher Herz Johannes R. Peham Pune N. Paqué Thomas Attin Joël S. Jenzer Philipp Körner Patrick R. Schmidlin Thomas Thurnheer Florian J. Wegehaupt Wendy E. Kaman Andrew Stubbs John P. Hays Viorel Rusu Alex Michie Thomas Binsl David Stejskal Michal Karpíšek Kai Bao Nagihan Bostanci Georgios N. Belibasakis Konstantinos Mitsakakis |
| author_sort |
Desirée Baumgartner |
| title |
OralDisk: A Chair-Side Compatible Molecular Platform Using Whole Saliva for Monitoring Oral Health at the Dental Practice |
| title_short |
OralDisk: A Chair-Side Compatible Molecular Platform Using Whole Saliva for Monitoring Oral Health at the Dental Practice |
| title_full |
OralDisk: A Chair-Side Compatible Molecular Platform Using Whole Saliva for Monitoring Oral Health at the Dental Practice |
| title_fullStr |
OralDisk: A Chair-Side Compatible Molecular Platform Using Whole Saliva for Monitoring Oral Health at the Dental Practice |
| title_full_unstemmed |
OralDisk: A Chair-Side Compatible Molecular Platform Using Whole Saliva for Monitoring Oral Health at the Dental Practice |
| title_sort |
oraldisk: a chair-side compatible molecular platform using whole saliva for monitoring oral health at the dental practice |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/99cf5461c1914ad484eb0d8614eb3dc2 |
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