Dry powder cationic lipopolymeric nanomicelle inhalation for targeted delivery of antitubercular drug to alveolar macrophage

Mithun Varghese Vadakkan,1 K Annapoorna,2 KC Sivakumar,3 Sathish Mundayoor,2 GS Vinod Kumar1 1Chemical Biology, 2Mycobacterium Research Group, 3Bioinformatics Facility, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India Abstract: Excipients having self-assembling properties are...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vadakkan MV, Annapoorna K, Sivakumar KC, Mundayoor S, Kumar GSV
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/99d849c436d14de1bca156f03ed3e3c6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:99d849c436d14de1bca156f03ed3e3c6
record_format	dspace
spelling	oai:doaj.org-article:99d849c436d14de1bca156f03ed3e3c62021-12-02T02:01:52ZDry powder cationic lipopolymeric nanomicelle inhalation for targeted delivery of antitubercular drug to alveolar macrophage1176-91141178-2013https://doaj.org/article/99d849c436d14de1bca156f03ed3e3c62013-08-01T00:00:00Zhttp://www.dovepress.com/dry-powder-cationic-lipopolymeric-nanomicelle-inhalation-for-targeted--a13939https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Mithun Varghese Vadakkan,1 K Annapoorna,2 KC Sivakumar,3 Sathish Mundayoor,2 GS Vinod Kumar1 1Chemical Biology, 2Mycobacterium Research Group, 3Bioinformatics Facility, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India Abstract: Excipients having self-assembling properties are less explored in the field of dry powder inhalation (DPI) technology. An amphiphilic lipopolymer system was developed using stearic acid (SA) and branched polyethyleneimine (BPEI) (1800 Dalton), at different proportions by covalent conjugation. A molecular dynamic (MD) simulation tool was employed for predicting the carrier behavior in a polar in vivo condition. The structural characterization was carried out using nuclear magnetic resonance spectroscopy (NMR) and Fourier transform infrared (FTIR) spectroscopy. The physical nature of the lipopolymer was analyzed by differential scanning calorimetry. Determination of zeta potential and diameter of the micelles showed existence of cationic particles in the nano size range when a lower number of primary amino groups of BPEI was grafted with SA. The rifampicin (RIF)-loaded lipopolymer was also formulated further into spray-dried microparticles. Powder X-ray diffraction (PXRD) studies revealed that the RIF API (active pharmaceutical ingredient) exists as molecular dispersion in spray-dried microparticles. Topological analysis of the spray-dried nanomicelle was carried out using scanning electron microscopy (SEM). A large population of the drug-carrying particles were found to be under the inhalable size range (fine particle fraction 67.88% ± 3%). In vitro drug release kinetics from spray-dried nanomicelles were carried out at lung fluid pH. Keywords: molecular dynamics, dry powder inhalation, pulmonary tuberculosis, nanomicelle, lipopolymer, rifampicinVadakkan MVAnnapoorna KSivakumar KCMundayoor SKumar GSVDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 2871-2885 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Vadakkan MV Annapoorna K Sivakumar KC Mundayoor S Kumar GSV Dry powder cationic lipopolymeric nanomicelle inhalation for targeted delivery of antitubercular drug to alveolar macrophage
description	Mithun Varghese Vadakkan,1 K Annapoorna,2 KC Sivakumar,3 Sathish Mundayoor,2 GS Vinod Kumar1 1Chemical Biology, 2Mycobacterium Research Group, 3Bioinformatics Facility, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India Abstract: Excipients having self-assembling properties are less explored in the field of dry powder inhalation (DPI) technology. An amphiphilic lipopolymer system was developed using stearic acid (SA) and branched polyethyleneimine (BPEI) (1800 Dalton), at different proportions by covalent conjugation. A molecular dynamic (MD) simulation tool was employed for predicting the carrier behavior in a polar in vivo condition. The structural characterization was carried out using nuclear magnetic resonance spectroscopy (NMR) and Fourier transform infrared (FTIR) spectroscopy. The physical nature of the lipopolymer was analyzed by differential scanning calorimetry. Determination of zeta potential and diameter of the micelles showed existence of cationic particles in the nano size range when a lower number of primary amino groups of BPEI was grafted with SA. The rifampicin (RIF)-loaded lipopolymer was also formulated further into spray-dried microparticles. Powder X-ray diffraction (PXRD) studies revealed that the RIF API (active pharmaceutical ingredient) exists as molecular dispersion in spray-dried microparticles. Topological analysis of the spray-dried nanomicelle was carried out using scanning electron microscopy (SEM). A large population of the drug-carrying particles were found to be under the inhalable size range (fine particle fraction 67.88% ± 3%). In vitro drug release kinetics from spray-dried nanomicelles were carried out at lung fluid pH. Keywords: molecular dynamics, dry powder inhalation, pulmonary tuberculosis, nanomicelle, lipopolymer, rifampicin
format	article
author	Vadakkan MV Annapoorna K Sivakumar KC Mundayoor S Kumar GSV
author_facet	Vadakkan MV Annapoorna K Sivakumar KC Mundayoor S Kumar GSV
author_sort	Vadakkan MV
title	Dry powder cationic lipopolymeric nanomicelle inhalation for targeted delivery of antitubercular drug to alveolar macrophage
title_short	Dry powder cationic lipopolymeric nanomicelle inhalation for targeted delivery of antitubercular drug to alveolar macrophage
title_full	Dry powder cationic lipopolymeric nanomicelle inhalation for targeted delivery of antitubercular drug to alveolar macrophage
title_fullStr	Dry powder cationic lipopolymeric nanomicelle inhalation for targeted delivery of antitubercular drug to alveolar macrophage
title_full_unstemmed	Dry powder cationic lipopolymeric nanomicelle inhalation for targeted delivery of antitubercular drug to alveolar macrophage
title_sort	dry powder cationic lipopolymeric nanomicelle inhalation for targeted delivery of antitubercular drug to alveolar macrophage
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/99d849c436d14de1bca156f03ed3e3c6
work_keys_str_mv	AT vadakkanmv drypowdercationiclipopolymericnanomicelleinhalationfortargeteddeliveryofantituberculardrugtoalveolarmacrophage AT annapoornak drypowdercationiclipopolymericnanomicelleinhalationfortargeteddeliveryofantituberculardrugtoalveolarmacrophage AT sivakumarkc drypowdercationiclipopolymericnanomicelleinhalationfortargeteddeliveryofantituberculardrugtoalveolarmacrophage AT mundayoors drypowdercationiclipopolymericnanomicelleinhalationfortargeteddeliveryofantituberculardrugtoalveolarmacrophage AT kumargsv drypowdercationiclipopolymericnanomicelleinhalationfortargeteddeliveryofantituberculardrugtoalveolarmacrophage
_version_	1718402792000847872

Dry powder cationic lipopolymeric nanomicelle inhalation for targeted delivery of antitubercular drug to alveolar macrophage

Ejemplares similares