Microbiota profile in mesophilic biodigestion of sugarcane vinasse in batch reactors

The vinasse is a residue of ethanol production with the potential for methane production, requiring an allochthonous inoculum. Several microorganisms act in the different phases of anaerobic digestion, and the identification of these microbial communities is essential to optimize the process. The ch...

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Autores principales:	Janaina Iltchenco, Vanessa Peruzzo, Flaviane Eva Magrini, Letícia Marconatto, Ana Paula Torres, Lademir Luiz Beal, Suelen Paesi
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	biogas methanobacterium sp. methanosaeta sp. microbial consortia sugarcane vinasse Environmental technology. Sanitary engineering TD1-1066
Acceso en línea:	https://doaj.org/article/99d9cc4e2fd3471fa3c2c10967b5223f
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spelling	oai:doaj.org-article:99d9cc4e2fd3471fa3c2c10967b5223f2021-11-06T11:26:26ZMicrobiota profile in mesophilic biodigestion of sugarcane vinasse in batch reactors0273-12231996-973210.2166/wst.2021.375https://doaj.org/article/99d9cc4e2fd3471fa3c2c10967b5223f2021-10-01T00:00:00Zhttp://wst.iwaponline.com/content/84/8/2028https://doaj.org/toc/0273-1223https://doaj.org/toc/1996-9732The vinasse is a residue of ethanol production with the potential for methane production, requiring an allochthonous inoculum. Several microorganisms act in the different phases of anaerobic digestion, and the identification of these microbial communities is essential to optimize the process. The characterization of the microbiota involved in the biodigestion of vinasse was observed in the initial stage (IS), at the peak of methane production (MS) and the end of the process (FS) of the best performance assay by high-throughput sequencing. The highest methane production was 0.78 mmolCH4.gVS.h−1 at 243.7 h in the substrate/inoculum ratio of 1.7, with consumption partial of acetic, propionic and isobutyric acids and an 82% reduction of chemical oxygen demand. High microbial diversity was found. The genera Clostridium, Acinetobacter, Candidatus Cloacamonas, Bacteroides, Syntrophomonas, Kosmotoga, the family Porphyromonadaceae and the class Bacteroidia were the most abundant in the maximum methane production. Methane production was driven by Methanobacterium and Methanosaeta, suggesting the metabolic pathways used were hydrogenotrophic and acetoclastic. HIGHLIGHTS The AD of vinasse with allochthonous inoculum reached 82% reduction in COD.; The increase of OLR provided the highest methanogenic activity (0.78 mmol CH4.gVS.h−1).; Clostridium, Candidatus Cloacamonas and Acinetobacter predominated in CH4 production.; Methanobacterium and Methanosaeta drove the methane production.;Janaina IltchencoVanessa PeruzzoFlaviane Eva MagriniLetícia MarconattoAna Paula TorresLademir Luiz BealSuelen PaesiIWA Publishingarticlebiogasmethanobacterium sp.methanosaeta sp.microbial consortiasugarcane vinasseEnvironmental technology. Sanitary engineeringTD1-1066ENWater Science and Technology, Vol 84, Iss 8, Pp 2028-2039 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biogas methanobacterium sp. methanosaeta sp. microbial consortia sugarcane vinasse Environmental technology. Sanitary engineering TD1-1066
spellingShingle	biogas methanobacterium sp. methanosaeta sp. microbial consortia sugarcane vinasse Environmental technology. Sanitary engineering TD1-1066 Janaina Iltchenco Vanessa Peruzzo Flaviane Eva Magrini Letícia Marconatto Ana Paula Torres Lademir Luiz Beal Suelen Paesi Microbiota profile in mesophilic biodigestion of sugarcane vinasse in batch reactors
description	The vinasse is a residue of ethanol production with the potential for methane production, requiring an allochthonous inoculum. Several microorganisms act in the different phases of anaerobic digestion, and the identification of these microbial communities is essential to optimize the process. The characterization of the microbiota involved in the biodigestion of vinasse was observed in the initial stage (IS), at the peak of methane production (MS) and the end of the process (FS) of the best performance assay by high-throughput sequencing. The highest methane production was 0.78 mmolCH4.gVS.h−1 at 243.7 h in the substrate/inoculum ratio of 1.7, with consumption partial of acetic, propionic and isobutyric acids and an 82% reduction of chemical oxygen demand. High microbial diversity was found. The genera Clostridium, Acinetobacter, Candidatus Cloacamonas, Bacteroides, Syntrophomonas, Kosmotoga, the family Porphyromonadaceae and the class Bacteroidia were the most abundant in the maximum methane production. Methane production was driven by Methanobacterium and Methanosaeta, suggesting the metabolic pathways used were hydrogenotrophic and acetoclastic. HIGHLIGHTS The AD of vinasse with allochthonous inoculum reached 82% reduction in COD.; The increase of OLR provided the highest methanogenic activity (0.78 mmol CH4.gVS.h−1).; Clostridium, Candidatus Cloacamonas and Acinetobacter predominated in CH4 production.; Methanobacterium and Methanosaeta drove the methane production.;
format	article
author	Janaina Iltchenco Vanessa Peruzzo Flaviane Eva Magrini Letícia Marconatto Ana Paula Torres Lademir Luiz Beal Suelen Paesi
author_facet	Janaina Iltchenco Vanessa Peruzzo Flaviane Eva Magrini Letícia Marconatto Ana Paula Torres Lademir Luiz Beal Suelen Paesi
author_sort	Janaina Iltchenco
title	Microbiota profile in mesophilic biodigestion of sugarcane vinasse in batch reactors
title_short	Microbiota profile in mesophilic biodigestion of sugarcane vinasse in batch reactors
title_full	Microbiota profile in mesophilic biodigestion of sugarcane vinasse in batch reactors
title_fullStr	Microbiota profile in mesophilic biodigestion of sugarcane vinasse in batch reactors
title_full_unstemmed	Microbiota profile in mesophilic biodigestion of sugarcane vinasse in batch reactors
title_sort	microbiota profile in mesophilic biodigestion of sugarcane vinasse in batch reactors
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/99d9cc4e2fd3471fa3c2c10967b5223f
work_keys_str_mv	AT janainailtchenco microbiotaprofileinmesophilicbiodigestionofsugarcanevinasseinbatchreactors AT vanessaperuzzo microbiotaprofileinmesophilicbiodigestionofsugarcanevinasseinbatchreactors AT flavianeevamagrini microbiotaprofileinmesophilicbiodigestionofsugarcanevinasseinbatchreactors AT leticiamarconatto microbiotaprofileinmesophilicbiodigestionofsugarcanevinasseinbatchreactors AT anapaulatorres microbiotaprofileinmesophilicbiodigestionofsugarcanevinasseinbatchreactors AT lademirluizbeal microbiotaprofileinmesophilicbiodigestionofsugarcanevinasseinbatchreactors AT suelenpaesi microbiotaprofileinmesophilicbiodigestionofsugarcanevinasseinbatchreactors
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Microbiota profile in mesophilic biodigestion of sugarcane vinasse in batch reactors

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