Lacrimal Gland Repair after Short-term Obstruction of Excretory Duct in Rabbits

Abstract Aqueous tear-deficient dry eye is a multifactorial chronic disorder in which the lacrimal glands fail to produce enough tears to maintain a healthy ocular surface. The existence of lacrimal gland stem/progenitor cells was proposed in several species, yet their origin and characteristics are...

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Autores principales:	Hui Lin, Ying Liu, Hong He, Benjamin Botsford, Samuel Yiu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/99e01fd3c92f470ab739b6f2bdd6d1f9
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spelling	oai:doaj.org-article:99e01fd3c92f470ab739b6f2bdd6d1f92021-12-02T16:06:58ZLacrimal Gland Repair after Short-term Obstruction of Excretory Duct in Rabbits10.1038/s41598-017-08197-22045-2322https://doaj.org/article/99e01fd3c92f470ab739b6f2bdd6d1f92017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08197-2https://doaj.org/toc/2045-2322Abstract Aqueous tear-deficient dry eye is a multifactorial chronic disorder in which the lacrimal glands fail to produce enough tears to maintain a healthy ocular surface. The existence of lacrimal gland stem/progenitor cells was proposed in several species, yet their origin and characteristics are not very clear. Here, we investigated the presence of resident progenitor cells and their regenerative potential in a rabbit model with lacrimal gland main excretory duct ligation-induced injury. The ligation-injured lacrimal glands temporarily decreased in weight and had impaired tear secretion. Protein expression profiles and transcriptional profiles were obtained from injured tissue. Isolated lacrimal gland progenitor cells were tested and characterized by stem cell-related marker evaluation, single cell clonal assay and three-dimensional (3-D) culture. The results of our study indicate that lacrimal glands are capable of tissue repair after duct ligation-induced injury, likely involving resident stem/progenitor cells and epithelial-mesenchymal transitions. Lacrimal gland progenitor cells isolated from ligated tissue can differentiate in 3-D culture. The results provide further insights into lacrimal gland stem/progenitor cell physiology and their potential for treating severe cases of tear deficiency.Hui LinYing LiuHong HeBenjamin BotsfordSamuel YiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hui Lin Ying Liu Hong He Benjamin Botsford Samuel Yiu Lacrimal Gland Repair after Short-term Obstruction of Excretory Duct in Rabbits
description	Abstract Aqueous tear-deficient dry eye is a multifactorial chronic disorder in which the lacrimal glands fail to produce enough tears to maintain a healthy ocular surface. The existence of lacrimal gland stem/progenitor cells was proposed in several species, yet their origin and characteristics are not very clear. Here, we investigated the presence of resident progenitor cells and their regenerative potential in a rabbit model with lacrimal gland main excretory duct ligation-induced injury. The ligation-injured lacrimal glands temporarily decreased in weight and had impaired tear secretion. Protein expression profiles and transcriptional profiles were obtained from injured tissue. Isolated lacrimal gland progenitor cells were tested and characterized by stem cell-related marker evaluation, single cell clonal assay and three-dimensional (3-D) culture. The results of our study indicate that lacrimal glands are capable of tissue repair after duct ligation-induced injury, likely involving resident stem/progenitor cells and epithelial-mesenchymal transitions. Lacrimal gland progenitor cells isolated from ligated tissue can differentiate in 3-D culture. The results provide further insights into lacrimal gland stem/progenitor cell physiology and their potential for treating severe cases of tear deficiency.
format	article
author	Hui Lin Ying Liu Hong He Benjamin Botsford Samuel Yiu
author_facet	Hui Lin Ying Liu Hong He Benjamin Botsford Samuel Yiu
author_sort	Hui Lin
title	Lacrimal Gland Repair after Short-term Obstruction of Excretory Duct in Rabbits
title_short	Lacrimal Gland Repair after Short-term Obstruction of Excretory Duct in Rabbits
title_full	Lacrimal Gland Repair after Short-term Obstruction of Excretory Duct in Rabbits
title_fullStr	Lacrimal Gland Repair after Short-term Obstruction of Excretory Duct in Rabbits
title_full_unstemmed	Lacrimal Gland Repair after Short-term Obstruction of Excretory Duct in Rabbits
title_sort	lacrimal gland repair after short-term obstruction of excretory duct in rabbits
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/99e01fd3c92f470ab739b6f2bdd6d1f9
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Lacrimal Gland Repair after Short-term Obstruction of Excretory Duct in Rabbits

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