Antibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection

Abstract Dengue is the most common mosquito-transmitted viral infection for which an improved vaccine is still needed. Although nonstructural protein-1 (NS1) immunization can protect mice against dengue infection, molecular mimicry between NS1 and host proteins makes NS1-based vaccines challenging t...

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Autores principales:	Yen-Chung Lai, Yung-Chun Chuang, Ching-Chuan Liu, Tzong-Shiann Ho, Yee-Shin Lin, Robert Anderson, Trai-Ming Yeh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/99e85865988b468bbb37fefb11e3201a
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spelling	oai:doaj.org-article:99e85865988b468bbb37fefb11e3201a2021-12-02T11:52:20ZAntibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection10.1038/s41598-017-07308-32045-2322https://doaj.org/article/99e85865988b468bbb37fefb11e3201a2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07308-3https://doaj.org/toc/2045-2322Abstract Dengue is the most common mosquito-transmitted viral infection for which an improved vaccine is still needed. Although nonstructural protein-1 (NS1) immunization can protect mice against dengue infection, molecular mimicry between NS1 and host proteins makes NS1-based vaccines challenging to develop. Based on the epitope recognized by the anti-NS1 monoclonal Ab (mAb) 33D2 which recognizes a conserved NS1 wing domain (NS1-WD) region but not host proteins, we synthesized a modified NS1-WD peptide to immunize mice. We found that both mAb 33D2 and modified NS1-WD peptide immune sera could induce complement-dependent lysis of dengue-infected but not un-infected cells in vitro. Furthermore, either active immunization with the modified NS1-WD peptide or passive transfer of mAb 33D2 efficiently protected mice against all serotypes of dengue virus infection. More importantly, dengue patients with more antibodies recognized the modified NS1-WD peptide had less severe disease. Thus, the modified NS1-WD peptide is a promising dengue vaccine candidate.Yen-Chung LaiYung-Chun ChuangChing-Chuan LiuTzong-Shiann HoYee-Shin LinRobert AndersonTrai-Ming YehNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yen-Chung Lai Yung-Chun Chuang Ching-Chuan Liu Tzong-Shiann Ho Yee-Shin Lin Robert Anderson Trai-Ming Yeh Antibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection
description	Abstract Dengue is the most common mosquito-transmitted viral infection for which an improved vaccine is still needed. Although nonstructural protein-1 (NS1) immunization can protect mice against dengue infection, molecular mimicry between NS1 and host proteins makes NS1-based vaccines challenging to develop. Based on the epitope recognized by the anti-NS1 monoclonal Ab (mAb) 33D2 which recognizes a conserved NS1 wing domain (NS1-WD) region but not host proteins, we synthesized a modified NS1-WD peptide to immunize mice. We found that both mAb 33D2 and modified NS1-WD peptide immune sera could induce complement-dependent lysis of dengue-infected but not un-infected cells in vitro. Furthermore, either active immunization with the modified NS1-WD peptide or passive transfer of mAb 33D2 efficiently protected mice against all serotypes of dengue virus infection. More importantly, dengue patients with more antibodies recognized the modified NS1-WD peptide had less severe disease. Thus, the modified NS1-WD peptide is a promising dengue vaccine candidate.
format	article
author	Yen-Chung Lai Yung-Chun Chuang Ching-Chuan Liu Tzong-Shiann Ho Yee-Shin Lin Robert Anderson Trai-Ming Yeh
author_facet	Yen-Chung Lai Yung-Chun Chuang Ching-Chuan Liu Tzong-Shiann Ho Yee-Shin Lin Robert Anderson Trai-Ming Yeh
author_sort	Yen-Chung Lai
title	Antibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection
title_short	Antibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection
title_full	Antibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection
title_fullStr	Antibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection
title_full_unstemmed	Antibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection
title_sort	antibodies against modified ns1 wing domain peptide protect against dengue virus infection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/99e85865988b468bbb37fefb11e3201a
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Antibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection

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