Genetic control of conventional and pheromone-stimulated biofilm formation in Candida albicans.

Candida albicans can stochastically switch between two phenotypes, white and opaque. Opaque cells are the sexually competent form of C. albicans and therefore undergo efficient polarized growth and mating in the presence of pheromone. In contrast, white cells cannot mate, but are induced - under a s...

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Autores principales:	Ching-Hsuan Lin, Shail Kabrawala, Emily P Fox, Clarissa J Nobile, Alexander D Johnson, Richard J Bennett
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/99f8335be0dd48869c44b77f410ee780
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spelling	oai:doaj.org-article:99f8335be0dd48869c44b77f410ee7802021-11-18T06:05:46ZGenetic control of conventional and pheromone-stimulated biofilm formation in Candida albicans.1553-73661553-737410.1371/journal.ppat.1003305https://doaj.org/article/99f8335be0dd48869c44b77f410ee7802013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23637598/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Candida albicans can stochastically switch between two phenotypes, white and opaque. Opaque cells are the sexually competent form of C. albicans and therefore undergo efficient polarized growth and mating in the presence of pheromone. In contrast, white cells cannot mate, but are induced - under a specialized set of conditions - to form biofilms in response to pheromone. In this work, we compare the genetic regulation of such "pheromone-stimulated" biofilms with that of "conventional" C. albicans biofilms. In particular, we examined a network of six transcriptional regulators (Bcr1, Brg1, Efg1, Tec1, Ndt80, and Rob1) that mediate conventional biofilm formation for their potential roles in pheromone-stimulated biofilm formation. We show that four of the six transcription factors (Bcr1, Brg1, Rob1, and Tec1) promote formation of both conventional and pheromone-stimulated biofilms, indicating they play general roles in cell cohesion and biofilm development. In addition, we identify the master transcriptional regulator of pheromone-stimulated biofilms as C. albicans Cph1, ortholog of Saccharomyces cerevisiae Ste12. Cph1 regulates mating in C. albicans opaque cells, and here we show that Cph1 is also essential for pheromone-stimulated biofilm formation in white cells. In contrast, Cph1 is dispensable for the formation of conventional biofilms. The regulation of pheromone- stimulated biofilm formation was further investigated by transcriptional profiling and genetic analyses. These studies identified 196 genes that are induced by pheromone signaling during biofilm formation. One of these genes, HGC1, is shown to be required for both conventional and pheromone-stimulated biofilm formation. Taken together, these observations compare and contrast the regulation of conventional and pheromone-stimulated biofilm formation in C. albicans, and demonstrate that Cph1 is required for the latter, but not the former.Ching-Hsuan LinShail KabrawalaEmily P FoxClarissa J NobileAlexander D JohnsonRichard J BennettPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 4, p e1003305 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Ching-Hsuan Lin Shail Kabrawala Emily P Fox Clarissa J Nobile Alexander D Johnson Richard J Bennett Genetic control of conventional and pheromone-stimulated biofilm formation in Candida albicans.
description	Candida albicans can stochastically switch between two phenotypes, white and opaque. Opaque cells are the sexually competent form of C. albicans and therefore undergo efficient polarized growth and mating in the presence of pheromone. In contrast, white cells cannot mate, but are induced - under a specialized set of conditions - to form biofilms in response to pheromone. In this work, we compare the genetic regulation of such "pheromone-stimulated" biofilms with that of "conventional" C. albicans biofilms. In particular, we examined a network of six transcriptional regulators (Bcr1, Brg1, Efg1, Tec1, Ndt80, and Rob1) that mediate conventional biofilm formation for their potential roles in pheromone-stimulated biofilm formation. We show that four of the six transcription factors (Bcr1, Brg1, Rob1, and Tec1) promote formation of both conventional and pheromone-stimulated biofilms, indicating they play general roles in cell cohesion and biofilm development. In addition, we identify the master transcriptional regulator of pheromone-stimulated biofilms as C. albicans Cph1, ortholog of Saccharomyces cerevisiae Ste12. Cph1 regulates mating in C. albicans opaque cells, and here we show that Cph1 is also essential for pheromone-stimulated biofilm formation in white cells. In contrast, Cph1 is dispensable for the formation of conventional biofilms. The regulation of pheromone- stimulated biofilm formation was further investigated by transcriptional profiling and genetic analyses. These studies identified 196 genes that are induced by pheromone signaling during biofilm formation. One of these genes, HGC1, is shown to be required for both conventional and pheromone-stimulated biofilm formation. Taken together, these observations compare and contrast the regulation of conventional and pheromone-stimulated biofilm formation in C. albicans, and demonstrate that Cph1 is required for the latter, but not the former.
format	article
author	Ching-Hsuan Lin Shail Kabrawala Emily P Fox Clarissa J Nobile Alexander D Johnson Richard J Bennett
author_facet	Ching-Hsuan Lin Shail Kabrawala Emily P Fox Clarissa J Nobile Alexander D Johnson Richard J Bennett
author_sort	Ching-Hsuan Lin
title	Genetic control of conventional and pheromone-stimulated biofilm formation in Candida albicans.
title_short	Genetic control of conventional and pheromone-stimulated biofilm formation in Candida albicans.
title_full	Genetic control of conventional and pheromone-stimulated biofilm formation in Candida albicans.
title_fullStr	Genetic control of conventional and pheromone-stimulated biofilm formation in Candida albicans.
title_full_unstemmed	Genetic control of conventional and pheromone-stimulated biofilm formation in Candida albicans.
title_sort	genetic control of conventional and pheromone-stimulated biofilm formation in candida albicans.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/99f8335be0dd48869c44b77f410ee780
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Genetic control of conventional and pheromone-stimulated biofilm formation in Candida albicans.

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