Phthalocyanine-loaded graphene nanoplatform for imaging-guided combinatorial phototherapy

Phthalocyanine-loaded graphene nanoplatform for imaging-guided combinatorial phototherapy

Olena Taratula,1 Mehulkumar Patel,2 Canan Schumann,1 Michael A Naleway,1 Addison J Pang,1 Huixin He,2 Oleh Taratula1 1Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Portland, OR, USA; 2Department of Chemistry, Rutgers University-Newark, Newark, NJ, USA Abstra...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Taratula O, Patel M, Schumann C, Naleway MA, Pang AJ, He H
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2015
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/9a01f11e3ec841c0b6ab533480460966
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:9a01f11e3ec841c0b6ab533480460966
record_format dspace
spelling oai:doaj.org-article:9a01f11e3ec841c0b6ab5334804609662021-12-02T05:09:55ZPhthalocyanine-loaded graphene nanoplatform for imaging-guided combinatorial phototherapy1178-2013https://doaj.org/article/9a01f11e3ec841c0b6ab5334804609662015-03-01T00:00:00Zhttp://www.dovepress.com/phthalocyanine-loaded-graphene-nanoplatform-fornbspimaging-guided-comb-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Olena Taratula,1 Mehulkumar Patel,2 Canan Schumann,1 Michael A Naleway,1 Addison J Pang,1 Huixin He,2 Oleh Taratula1 1Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Portland, OR, USA; 2Department of Chemistry, Rutgers University-Newark, Newark, NJ, USA Abstract: We report a novel cancer-targeted nanomedicine platform for imaging and prospect for future treatment of unresected ovarian cancer tumors by intraoperative multimodal phototherapy. To develop the required theranostic system, novel low-oxygen graphene nanosheets were chemically modified with polypropylenimine dendrimers loaded with phthalocyanine (Pc) as a photosensitizer. Such a molecular design prevents fluorescence quenching of the Pc by graphene nanosheets, providing the possibility of fluorescence imaging. Furthermore, the developed nanoplatform was conjugated with poly(ethylene glycol), to improve biocompatibility, and with luteinizing hormone-releasing hormone (LHRH) peptide, for tumor-targeted delivery. Notably, a low-power near-infrared (NIR) irradiation of single wavelength was used for both heat generation by the graphene nanosheets (photothermal therapy [PTT]) and for reactive oxygen species (ROS)-production by Pc (photodynamic therapy [PDT]). The combinatorial phototherapy resulted in an enhanced destruction of ovarian cancer cells, with a killing efficacy of 90%–95% at low Pc and low-oxygen graphene dosages, presumably conferring cytotoxicity to the synergistic effects of generated ROS and mild hyperthermia. An animal study confirmed that Pc loaded into the nanoplatform can be employed as a NIR fluorescence agent for imaging-guided drug delivery. Hence, the newly developed Pc-graphene nanoplatform has the significant potential as an effective NIR theranostic probe for imaging and combinatorial phototherapy. Keywords: graphene nanosheets, phthalocyanine, photothermal therapy, photodynamic therapy, theranostic Taratula OPatel MSchumann CNaleway MAPang AJHe HTaratula ODove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 2347-2362 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Taratula O
Patel M
Schumann C
Naleway MA
Pang AJ
He H
Taratula O
Phthalocyanine-loaded graphene nanoplatform for imaging-guided combinatorial phototherapy
description Olena Taratula,1 Mehulkumar Patel,2 Canan Schumann,1 Michael A Naleway,1 Addison J Pang,1 Huixin He,2 Oleh Taratula1 1Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Portland, OR, USA; 2Department of Chemistry, Rutgers University-Newark, Newark, NJ, USA Abstract: We report a novel cancer-targeted nanomedicine platform for imaging and prospect for future treatment of unresected ovarian cancer tumors by intraoperative multimodal phototherapy. To develop the required theranostic system, novel low-oxygen graphene nanosheets were chemically modified with polypropylenimine dendrimers loaded with phthalocyanine (Pc) as a photosensitizer. Such a molecular design prevents fluorescence quenching of the Pc by graphene nanosheets, providing the possibility of fluorescence imaging. Furthermore, the developed nanoplatform was conjugated with poly(ethylene glycol), to improve biocompatibility, and with luteinizing hormone-releasing hormone (LHRH) peptide, for tumor-targeted delivery. Notably, a low-power near-infrared (NIR) irradiation of single wavelength was used for both heat generation by the graphene nanosheets (photothermal therapy [PTT]) and for reactive oxygen species (ROS)-production by Pc (photodynamic therapy [PDT]). The combinatorial phototherapy resulted in an enhanced destruction of ovarian cancer cells, with a killing efficacy of 90%–95% at low Pc and low-oxygen graphene dosages, presumably conferring cytotoxicity to the synergistic effects of generated ROS and mild hyperthermia. An animal study confirmed that Pc loaded into the nanoplatform can be employed as a NIR fluorescence agent for imaging-guided drug delivery. Hence, the newly developed Pc-graphene nanoplatform has the significant potential as an effective NIR theranostic probe for imaging and combinatorial phototherapy. Keywords: graphene nanosheets, phthalocyanine, photothermal therapy, photodynamic therapy, theranostic 
format article
author Taratula O
Patel M
Schumann C
Naleway MA
Pang AJ
He H
Taratula O
author_facet Taratula O
Patel M
Schumann C
Naleway MA
Pang AJ
He H
Taratula O
author_sort Taratula O
title Phthalocyanine-loaded graphene nanoplatform for imaging-guided combinatorial phototherapy
title_short Phthalocyanine-loaded graphene nanoplatform for imaging-guided combinatorial phototherapy
title_full Phthalocyanine-loaded graphene nanoplatform for imaging-guided combinatorial phototherapy
title_fullStr Phthalocyanine-loaded graphene nanoplatform for imaging-guided combinatorial phototherapy
title_full_unstemmed Phthalocyanine-loaded graphene nanoplatform for imaging-guided combinatorial phototherapy
title_sort phthalocyanine-loaded graphene nanoplatform for imaging-guided combinatorial phototherapy
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/9a01f11e3ec841c0b6ab533480460966
work_keys_str_mv AT taratulao phthalocyanineloadedgraphenenanoplatformfornbspimagingguidedcombinatorialphototherapy
AT patelm phthalocyanineloadedgraphenenanoplatformfornbspimagingguidedcombinatorialphototherapy
AT schumannc phthalocyanineloadedgraphenenanoplatformfornbspimagingguidedcombinatorialphototherapy
AT nalewayma phthalocyanineloadedgraphenenanoplatformfornbspimagingguidedcombinatorialphototherapy
AT pangaj phthalocyanineloadedgraphenenanoplatformfornbspimagingguidedcombinatorialphototherapy
AT heh phthalocyanineloadedgraphenenanoplatformfornbspimagingguidedcombinatorialphototherapy
AT taratulao phthalocyanineloadedgraphenenanoplatformfornbspimagingguidedcombinatorialphototherapy
_version_ 1718400546601172992

Ejemplares similares