Intercellular transfer of oncogenic H-Ras at the immunological synapse.

Immune cells establish dynamic adhesive cell-cell interactions at a specific contact region, termed the immunological synapse (IS). Intriguing features of the IS are the formation of regions of plasma membrane fusion and the intercellular exchange of membrane fragments between the conjugated cells....

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Autores principales:	Oded Rechavi, Itamar Goldstein, Helly Vernitsky, Barak Rotblat, Yoel Kloog
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2007
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9a067d86b8d440899a275fa5ddd22ddf
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spelling	oai:doaj.org-article:9a067d86b8d440899a275fa5ddd22ddf2021-11-25T06:13:50ZIntercellular transfer of oncogenic H-Ras at the immunological synapse.1932-620310.1371/journal.pone.0001204https://doaj.org/article/9a067d86b8d440899a275fa5ddd22ddf2007-11-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0001204https://doaj.org/toc/1932-6203Immune cells establish dynamic adhesive cell-cell interactions at a specific contact region, termed the immunological synapse (IS). Intriguing features of the IS are the formation of regions of plasma membrane fusion and the intercellular exchange of membrane fragments between the conjugated cells. It is not known whether upon IS formation, intact intracellular proteins can transfer from target cells to lymphocytes to allow the transmission of signals across cell boundaries. Here we show by both FACS and confocal microscopy that human lymphocytes acquire from the cells they scan the inner-membrane protein H-Ras, a G-protein vital for common lymphocyte functions and a prominent participant in human cancer. The transfer was cell contact-dependent and occurred in the context of cell-conjugate formation. Moreover, the acquisition of oncogenic H-RasG12V by natural killer (NK) and T lymphocytes had important biological functions in the adopting lymphocytes: the transferred H-RasG12V induced ERK phosphorylation, increased interferon-gamma and tumor necrosis factor-alpha secretion, enhanced lymphocyte proliferation, and augmented NK-mediated target cell killing. Our findings reveal a novel mode of cell-to-cell communication-allowing lymphocytes to extend the confines of their own proteome-which may moreover play an important role in natural tumor immunity.Oded RechaviItamar GoldsteinHelly VernitskyBarak RotblatYoel KloogPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 2, Iss 11, p e1204 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Oded Rechavi Itamar Goldstein Helly Vernitsky Barak Rotblat Yoel Kloog Intercellular transfer of oncogenic H-Ras at the immunological synapse.
description	Immune cells establish dynamic adhesive cell-cell interactions at a specific contact region, termed the immunological synapse (IS). Intriguing features of the IS are the formation of regions of plasma membrane fusion and the intercellular exchange of membrane fragments between the conjugated cells. It is not known whether upon IS formation, intact intracellular proteins can transfer from target cells to lymphocytes to allow the transmission of signals across cell boundaries. Here we show by both FACS and confocal microscopy that human lymphocytes acquire from the cells they scan the inner-membrane protein H-Ras, a G-protein vital for common lymphocyte functions and a prominent participant in human cancer. The transfer was cell contact-dependent and occurred in the context of cell-conjugate formation. Moreover, the acquisition of oncogenic H-RasG12V by natural killer (NK) and T lymphocytes had important biological functions in the adopting lymphocytes: the transferred H-RasG12V induced ERK phosphorylation, increased interferon-gamma and tumor necrosis factor-alpha secretion, enhanced lymphocyte proliferation, and augmented NK-mediated target cell killing. Our findings reveal a novel mode of cell-to-cell communication-allowing lymphocytes to extend the confines of their own proteome-which may moreover play an important role in natural tumor immunity.
format	article
author	Oded Rechavi Itamar Goldstein Helly Vernitsky Barak Rotblat Yoel Kloog
author_facet	Oded Rechavi Itamar Goldstein Helly Vernitsky Barak Rotblat Yoel Kloog
author_sort	Oded Rechavi
title	Intercellular transfer of oncogenic H-Ras at the immunological synapse.
title_short	Intercellular transfer of oncogenic H-Ras at the immunological synapse.
title_full	Intercellular transfer of oncogenic H-Ras at the immunological synapse.
title_fullStr	Intercellular transfer of oncogenic H-Ras at the immunological synapse.
title_full_unstemmed	Intercellular transfer of oncogenic H-Ras at the immunological synapse.
title_sort	intercellular transfer of oncogenic h-ras at the immunological synapse.
publisher	Public Library of Science (PLoS)
publishDate	2007
url	https://doaj.org/article/9a067d86b8d440899a275fa5ddd22ddf
work_keys_str_mv	AT odedrechavi intercellulartransferofoncogenichrasattheimmunologicalsynapse AT itamargoldstein intercellulartransferofoncogenichrasattheimmunologicalsynapse AT hellyvernitsky intercellulartransferofoncogenichrasattheimmunologicalsynapse AT barakrotblat intercellulartransferofoncogenichrasattheimmunologicalsynapse AT yoelkloog intercellulartransferofoncogenichrasattheimmunologicalsynapse
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Intercellular transfer of oncogenic H-Ras at the immunological synapse.

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