Novel Insights and Features of the NDM-5-Producing <named-content content-type="genus-species">Escherichia coli</named-content> Sequence Type 167 High-Risk Clone

ABSTRACT Escherichia coli sequence type 167 (ST167), producing the metallo beta-lactamase NDM-5, has been isolated as a colonizer of patients recovered at the University Hospital Policlinico Umberto I of Rome. Phylogenesis and comparative analysis of the genomes of these strains were performed again...

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Autores principales:	Aurora Garcia-Fernandez, Laura Villa, Giulia Bibbolino, Alessia Bressan, Maria Trancassini, Valeria Pietropaolo, Mario Venditti, Guido Antonelli, Alessandra Carattoli
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Escherichia coli capsular polysaccharide carbapenems Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/9a0d4e3ee34e45649343f8b5cc5ea3bd
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spelling	oai:doaj.org-article:9a0d4e3ee34e45649343f8b5cc5ea3bd2021-11-15T15:29:17ZNovel Insights and Features of the NDM-5-Producing <named-content content-type="genus-species">Escherichia coli</named-content> Sequence Type 167 High-Risk Clone10.1128/mSphere.00269-202379-5042https://doaj.org/article/9a0d4e3ee34e45649343f8b5cc5ea3bd2020-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00269-20https://doaj.org/toc/2379-5042ABSTRACT Escherichia coli sequence type 167 (ST167), producing the metallo beta-lactamase NDM-5, has been isolated as a colonizer of patients recovered at the University Hospital Policlinico Umberto I of Rome. Phylogenesis and comparative analysis of the genomes of these strains were performed against 343 ST167 genomes available from the EnteroBase database. These analyses revealed that resistance plasmids, integrative conjugative elements (ICEs), carrying the yersiniabactin virulence trait and capsular synthesis gene clusters had variable compositions and distributions within different strains of the ST167 clone. A novel capsular synthesis gene cluster, highly similar to the K48 cluster previously described only for Klebsiella pneumoniae, was identified in phylogenetically related strains of the ST167 clone. IMPORTANCE Global dissemination of some E. coli high-risk clones has been described in the last decades. The most widespread was the ST131 clone, associated with extended-spectrum-beta-lactamase (ESBL) production. Genomics of ST131 demonstrated that one clade within the ST emerged in the early 2000s, followed by a rapid, global expansion. The E. coli ST167 clone is emerging throughout the world, being frequently reported for its association with carbapenem resistance. Our study shows that virulence features are differently represented within the ST167 population. One clade shows the K48 capsular synthesis gene cluster of K. pneumoniae, not previously described for E. coli, and is populated by NDM-5-producing strains. The combination of resistance and virulence may sustain the global expansion of this specific ST167 clade.Aurora Garcia-FernandezLaura VillaGiulia BibbolinoAlessia BressanMaria TrancassiniValeria PietropaoloMario VendittiGuido AntonelliAlessandra CarattoliAmerican Society for MicrobiologyarticleEscherichia colicapsular polysaccharidecarbapenemsMicrobiologyQR1-502ENmSphere, Vol 5, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Escherichia coli capsular polysaccharide carbapenems Microbiology QR1-502
spellingShingle	Escherichia coli capsular polysaccharide carbapenems Microbiology QR1-502 Aurora Garcia-Fernandez Laura Villa Giulia Bibbolino Alessia Bressan Maria Trancassini Valeria Pietropaolo Mario Venditti Guido Antonelli Alessandra Carattoli Novel Insights and Features of the NDM-5-Producing <named-content content-type="genus-species">Escherichia coli</named-content> Sequence Type 167 High-Risk Clone
description	ABSTRACT Escherichia coli sequence type 167 (ST167), producing the metallo beta-lactamase NDM-5, has been isolated as a colonizer of patients recovered at the University Hospital Policlinico Umberto I of Rome. Phylogenesis and comparative analysis of the genomes of these strains were performed against 343 ST167 genomes available from the EnteroBase database. These analyses revealed that resistance plasmids, integrative conjugative elements (ICEs), carrying the yersiniabactin virulence trait and capsular synthesis gene clusters had variable compositions and distributions within different strains of the ST167 clone. A novel capsular synthesis gene cluster, highly similar to the K48 cluster previously described only for Klebsiella pneumoniae, was identified in phylogenetically related strains of the ST167 clone. IMPORTANCE Global dissemination of some E. coli high-risk clones has been described in the last decades. The most widespread was the ST131 clone, associated with extended-spectrum-beta-lactamase (ESBL) production. Genomics of ST131 demonstrated that one clade within the ST emerged in the early 2000s, followed by a rapid, global expansion. The E. coli ST167 clone is emerging throughout the world, being frequently reported for its association with carbapenem resistance. Our study shows that virulence features are differently represented within the ST167 population. One clade shows the K48 capsular synthesis gene cluster of K. pneumoniae, not previously described for E. coli, and is populated by NDM-5-producing strains. The combination of resistance and virulence may sustain the global expansion of this specific ST167 clade.
format	article
author	Aurora Garcia-Fernandez Laura Villa Giulia Bibbolino Alessia Bressan Maria Trancassini Valeria Pietropaolo Mario Venditti Guido Antonelli Alessandra Carattoli
author_facet	Aurora Garcia-Fernandez Laura Villa Giulia Bibbolino Alessia Bressan Maria Trancassini Valeria Pietropaolo Mario Venditti Guido Antonelli Alessandra Carattoli
author_sort	Aurora Garcia-Fernandez
title	Novel Insights and Features of the NDM-5-Producing <named-content content-type="genus-species">Escherichia coli</named-content> Sequence Type 167 High-Risk Clone
title_short	Novel Insights and Features of the NDM-5-Producing <named-content content-type="genus-species">Escherichia coli</named-content> Sequence Type 167 High-Risk Clone
title_full	Novel Insights and Features of the NDM-5-Producing <named-content content-type="genus-species">Escherichia coli</named-content> Sequence Type 167 High-Risk Clone
title_fullStr	Novel Insights and Features of the NDM-5-Producing <named-content content-type="genus-species">Escherichia coli</named-content> Sequence Type 167 High-Risk Clone
title_full_unstemmed	Novel Insights and Features of the NDM-5-Producing <named-content content-type="genus-species">Escherichia coli</named-content> Sequence Type 167 High-Risk Clone
title_sort	novel insights and features of the ndm-5-producing <named-content content-type="genus-species">escherichia coli</named-content> sequence type 167 high-risk clone
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/9a0d4e3ee34e45649343f8b5cc5ea3bd
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Novel Insights and Features of the NDM-5-Producing <named-content content-type="genus-species">Escherichia coli</named-content> Sequence Type 167 High-Risk Clone

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