Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats

Zhengwang Cao,1 Yiliang Fang,1 Yonghui Lu,1 Fenghua Qian,2 Qinglong Ma,1 Mingdi He,1 Huifeng Pi,1 Zhengping Yu,1 Zhou Zhou1 1Department of Occupational Health, 2Department of Haematology, Southwest Hospital, Third Military Medical University, Chongqing, People’s Republic of China Abstrac...

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Autores principales:	Cao Z, Fang Y, Lu Y, Qian F, Ma Q, He M, Pi H, Yu Z, Zhou Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	NLRP3 inflammasome nickel oxide nanoparticles inflammation cytokines Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9a0e78d8de1e448e953ee4fa7ec0edb5
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spelling	oai:doaj.org-article:9a0e78d8de1e448e953ee4fa7ec0edb52021-12-02T06:34:32ZExposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats1178-2013https://doaj.org/article/9a0e78d8de1e448e953ee4fa7ec0edb52016-07-01T00:00:00Zhttps://www.dovepress.com/exposure-to-nickel-oxide-nanoparticles-induces-pulmonary-inflammation--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zhengwang Cao,1 Yiliang Fang,1 Yonghui Lu,1 Fenghua Qian,2 Qinglong Ma,1 Mingdi He,1 Huifeng Pi,1 Zhengping Yu,1 Zhou Zhou1 1Department of Occupational Health, 2Department of Haematology, Southwest Hospital, Third Military Medical University, Chongqing, People’s Republic of China Abstract: With recent advances in the manufacture and application of nickel oxide nanoparticles (NiONPs), concerns about their adverse effects on the respiratory system are increasing. However, the underlying cellular and molecular mechanisms of NiONP-induced pulmonary toxicity remain unclear. In this study, we focused on the impacts of NiONPs on pulmonary inflammation and investigated whether the NLRP3 inflammasome is involved in NiONP-induced pulmonary inflammation and injury. NiONP suspensions were administered by single intratracheal instillation to rats, and inflammatory responses were evaluated at 3 days, 7 days, or 28 days after treatment. NiONP exposure resulted in sustained pulmonary inflammation accompanied by inflammatory cell infiltration, alveolar proteinosis, and cytokine secretion. Expression of Nlrp3 was markedly upregulated by the NiONPs, which was accompanied by overexpression of the active form of caspase-1 (p20) and interleukin (IL)-1β secretion in vivo. NiONP-induced IL-1β secretion was partially prevented by co-treatment with a caspase-1 inhibitor in macrophages. Moreover, siRNA-mediated Nlrp3 knockdown completely attenuated NiONP-induced cytokine release and caspase-1 activity in macrophages in vitro. In addition, NiONP-induced NLRP3 inflammasome activation requires particle uptake and reactive oxygen species production. Collectively, our findings suggest that the NLRP3 inflammasome participates in NiONP-induced pulmonary inflammation and offer new strategies to combat the pulmonary toxicity induced by NiONPs. Keywords: NLRP3 inflammasome, nickel oxide nanoparticles, inflammation, cytokinesCao ZFang YLu YQian FMa QHe MPi HYu ZZhou ZDove Medical PressarticleNLRP3 inflammasomenickel oxide nanoparticlesinflammationcytokinesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 3331-3346 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	NLRP3 inflammasome nickel oxide nanoparticles inflammation cytokines Medicine (General) R5-920
spellingShingle	NLRP3 inflammasome nickel oxide nanoparticles inflammation cytokines Medicine (General) R5-920 Cao Z Fang Y Lu Y Qian F Ma Q He M Pi H Yu Z Zhou Z Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats
description	Zhengwang Cao,1 Yiliang Fang,1 Yonghui Lu,1 Fenghua Qian,2 Qinglong Ma,1 Mingdi He,1 Huifeng Pi,1 Zhengping Yu,1 Zhou Zhou1 1Department of Occupational Health, 2Department of Haematology, Southwest Hospital, Third Military Medical University, Chongqing, People’s Republic of China Abstract: With recent advances in the manufacture and application of nickel oxide nanoparticles (NiONPs), concerns about their adverse effects on the respiratory system are increasing. However, the underlying cellular and molecular mechanisms of NiONP-induced pulmonary toxicity remain unclear. In this study, we focused on the impacts of NiONPs on pulmonary inflammation and investigated whether the NLRP3 inflammasome is involved in NiONP-induced pulmonary inflammation and injury. NiONP suspensions were administered by single intratracheal instillation to rats, and inflammatory responses were evaluated at 3 days, 7 days, or 28 days after treatment. NiONP exposure resulted in sustained pulmonary inflammation accompanied by inflammatory cell infiltration, alveolar proteinosis, and cytokine secretion. Expression of Nlrp3 was markedly upregulated by the NiONPs, which was accompanied by overexpression of the active form of caspase-1 (p20) and interleukin (IL)-1β secretion in vivo. NiONP-induced IL-1β secretion was partially prevented by co-treatment with a caspase-1 inhibitor in macrophages. Moreover, siRNA-mediated Nlrp3 knockdown completely attenuated NiONP-induced cytokine release and caspase-1 activity in macrophages in vitro. In addition, NiONP-induced NLRP3 inflammasome activation requires particle uptake and reactive oxygen species production. Collectively, our findings suggest that the NLRP3 inflammasome participates in NiONP-induced pulmonary inflammation and offer new strategies to combat the pulmonary toxicity induced by NiONPs. Keywords: NLRP3 inflammasome, nickel oxide nanoparticles, inflammation, cytokines
format	article
author	Cao Z Fang Y Lu Y Qian F Ma Q He M Pi H Yu Z Zhou Z
author_facet	Cao Z Fang Y Lu Y Qian F Ma Q He M Pi H Yu Z Zhou Z
author_sort	Cao Z
title	Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats
title_short	Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats
title_full	Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats
title_fullStr	Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats
title_full_unstemmed	Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats
title_sort	exposure to nickel oxide nanoparticles induces pulmonary inflammation through nlrp3 inflammasome activation in rats
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/9a0e78d8de1e448e953ee4fa7ec0edb5
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Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats

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