Detection of viral proteins in human cells lines by xeno-proteomics: elimination of the last valid excuse for not testing every cellular proteome dataset for viral proteins.

Cell cultures used routinely in proteomic experiments may contain proteins from other species because of infection, transfection or just contamination. Since infection or contamination may affect the results of a biological experiment, it is important to test the samples for the presence of "al...

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Autores principales:	Alexey L Chernobrovkin, Roman A Zubarev
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9a1382e2544c4455bbb799824c45999d
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spelling	oai:doaj.org-article:9a1382e2544c4455bbb799824c45999d2021-11-18T08:28:48ZDetection of viral proteins in human cells lines by xeno-proteomics: elimination of the last valid excuse for not testing every cellular proteome dataset for viral proteins.1932-620310.1371/journal.pone.0091433https://doaj.org/article/9a1382e2544c4455bbb799824c45999d2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24618588/?tool=EBIhttps://doaj.org/toc/1932-6203Cell cultures used routinely in proteomic experiments may contain proteins from other species because of infection, transfection or just contamination. Since infection or contamination may affect the results of a biological experiment, it is important to test the samples for the presence of "alien" proteins. Usually cells are tested only for the most common infections, and most of the existing tests are targeting specific contaminations. Here we describe a three-step procedure for reliable untargeted detection of viral proteins using proteomics data, and recommend this or similar procedure to be applied to every proteomics dataset submitted for publication.Alexey L ChernobrovkinRoman A ZubarevPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e91433 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexey L Chernobrovkin Roman A Zubarev Detection of viral proteins in human cells lines by xeno-proteomics: elimination of the last valid excuse for not testing every cellular proteome dataset for viral proteins.
description	Cell cultures used routinely in proteomic experiments may contain proteins from other species because of infection, transfection or just contamination. Since infection or contamination may affect the results of a biological experiment, it is important to test the samples for the presence of "alien" proteins. Usually cells are tested only for the most common infections, and most of the existing tests are targeting specific contaminations. Here we describe a three-step procedure for reliable untargeted detection of viral proteins using proteomics data, and recommend this or similar procedure to be applied to every proteomics dataset submitted for publication.
format	article
author	Alexey L Chernobrovkin Roman A Zubarev
author_facet	Alexey L Chernobrovkin Roman A Zubarev
author_sort	Alexey L Chernobrovkin
title	Detection of viral proteins in human cells lines by xeno-proteomics: elimination of the last valid excuse for not testing every cellular proteome dataset for viral proteins.
title_short	Detection of viral proteins in human cells lines by xeno-proteomics: elimination of the last valid excuse for not testing every cellular proteome dataset for viral proteins.
title_full	Detection of viral proteins in human cells lines by xeno-proteomics: elimination of the last valid excuse for not testing every cellular proteome dataset for viral proteins.
title_fullStr	Detection of viral proteins in human cells lines by xeno-proteomics: elimination of the last valid excuse for not testing every cellular proteome dataset for viral proteins.
title_full_unstemmed	Detection of viral proteins in human cells lines by xeno-proteomics: elimination of the last valid excuse for not testing every cellular proteome dataset for viral proteins.
title_sort	detection of viral proteins in human cells lines by xeno-proteomics: elimination of the last valid excuse for not testing every cellular proteome dataset for viral proteins.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/9a1382e2544c4455bbb799824c45999d
work_keys_str_mv	AT alexeylchernobrovkin detectionofviralproteinsinhumancellslinesbyxenoproteomicseliminationofthelastvalidexcusefornottestingeverycellularproteomedatasetforviralproteins AT romanazubarev detectionofviralproteinsinhumancellslinesbyxenoproteomicseliminationofthelastvalidexcusefornottestingeverycellularproteomedatasetforviralproteins
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Detection of viral proteins in human cells lines by xeno-proteomics: elimination of the last valid excuse for not testing every cellular proteome dataset for viral proteins.

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