Construction of an Acetate Metabolic Pathway to Enhance Electron Generation of Engineered Shewanella oneidensis
Background: Microbial fuel cells (MFCs) are a novel bioelectrochemical devices that can use exoelectrogens as biocatalyst to convert various organic wastes into electricity. Among them, acetate, a major component of industrial biological wastewater and by-product of lignocellulose degradation, could...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Frontiers Media S.A.
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/9a1a60fe777344fdafbe7ffd50820224 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:9a1a60fe777344fdafbe7ffd50820224 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:9a1a60fe777344fdafbe7ffd508202242021-11-19T06:03:37ZConstruction of an Acetate Metabolic Pathway to Enhance Electron Generation of Engineered Shewanella oneidensis2296-418510.3389/fbioe.2021.757953https://doaj.org/article/9a1a60fe777344fdafbe7ffd508202242021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.757953/fullhttps://doaj.org/toc/2296-4185Background: Microbial fuel cells (MFCs) are a novel bioelectrochemical devices that can use exoelectrogens as biocatalyst to convert various organic wastes into electricity. Among them, acetate, a major component of industrial biological wastewater and by-product of lignocellulose degradation, could release eight electrons per mole when completely degraded into CO2 and H2O, which has been identified as a promising carbon source and electron donor. However, Shewanella oneidensis MR-1, a famous facultative anaerobic exoelectrogens, only preferentially uses lactate as carbon source and electron donor and could hardly metabolize acetate in MFCs, which greatly limited Coulombic efficiency of MFCs and the capacity of bio-catalysis.Results: Here, to enable acetate as the sole carbon source and electron donor for electricity production in S. oneidensis, we successfully constructed three engineered S. oneidensis (named AceU1, AceU2, and AceU3) by assembling the succinyl-CoA:acetate CoA-transferase (SCACT) metabolism pathways, including acetate coenzyme A transferase encoded by ato1 and ato2 gene from G. sulfurreducens and citrate synthase encoded by the gltA gene from S. oneidensis, which could successfully utilize acetate as carbon source under anaerobic and aerobic conditions. Then, biochemical characterizations showed the engineered strain AceU3 generated a maximum power density of 8.3 ± 1.2 mW/m2 with acetate as the sole electron donor in MFCs. In addition, when further using lactate as the electron donor, the maximum power density obtained by AceU3 was 51.1 ± 3.1 mW/m2, which approximately 2.4-fold higher than that of wild type (WT). Besides, the Coulombic efficiency of AceU3 strain could reach 12.4% increased by 2.0-fold compared that of WT, which demonstrated that the engineered strain AceU3 can further utilize acetate as an electron donor to continuously generate electricity.Conclusion: In the present study, we first rationally designed S. oneidensis for enhancing the electron generation by using acetate as sole carbon source and electron donor. Based on synthetic biology strategies, modular assembly of acetate metabolic pathways could be further extended to other exoelectrogens to improve the Coulombic efficiency and broaden the spectrum of available carbon sources in MFCs for bioelectricity production.Junqi ZhangJunqi ZhangZheng ChenZheng ChenChangjiang LiuChangjiang LiuJianxun LiXingjuan AnXingjuan AnDeguang WuXi SunBaocai ZhangBaocai ZhangLongping FuFeng LiFeng LiHao SongHao SongFrontiers Media S.A.articleacetatemicrobial fuel cellsynthetic biologyShewanella oneidensiscoulombic efficiencyBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
acetate microbial fuel cell synthetic biology Shewanella oneidensis coulombic efficiency Biotechnology TP248.13-248.65 |
| spellingShingle |
acetate microbial fuel cell synthetic biology Shewanella oneidensis coulombic efficiency Biotechnology TP248.13-248.65 Junqi Zhang Junqi Zhang Zheng Chen Zheng Chen Changjiang Liu Changjiang Liu Jianxun Li Xingjuan An Xingjuan An Deguang Wu Xi Sun Baocai Zhang Baocai Zhang Longping Fu Feng Li Feng Li Hao Song Hao Song Construction of an Acetate Metabolic Pathway to Enhance Electron Generation of Engineered Shewanella oneidensis |
| description |
Background: Microbial fuel cells (MFCs) are a novel bioelectrochemical devices that can use exoelectrogens as biocatalyst to convert various organic wastes into electricity. Among them, acetate, a major component of industrial biological wastewater and by-product of lignocellulose degradation, could release eight electrons per mole when completely degraded into CO2 and H2O, which has been identified as a promising carbon source and electron donor. However, Shewanella oneidensis MR-1, a famous facultative anaerobic exoelectrogens, only preferentially uses lactate as carbon source and electron donor and could hardly metabolize acetate in MFCs, which greatly limited Coulombic efficiency of MFCs and the capacity of bio-catalysis.Results: Here, to enable acetate as the sole carbon source and electron donor for electricity production in S. oneidensis, we successfully constructed three engineered S. oneidensis (named AceU1, AceU2, and AceU3) by assembling the succinyl-CoA:acetate CoA-transferase (SCACT) metabolism pathways, including acetate coenzyme A transferase encoded by ato1 and ato2 gene from G. sulfurreducens and citrate synthase encoded by the gltA gene from S. oneidensis, which could successfully utilize acetate as carbon source under anaerobic and aerobic conditions. Then, biochemical characterizations showed the engineered strain AceU3 generated a maximum power density of 8.3 ± 1.2 mW/m2 with acetate as the sole electron donor in MFCs. In addition, when further using lactate as the electron donor, the maximum power density obtained by AceU3 was 51.1 ± 3.1 mW/m2, which approximately 2.4-fold higher than that of wild type (WT). Besides, the Coulombic efficiency of AceU3 strain could reach 12.4% increased by 2.0-fold compared that of WT, which demonstrated that the engineered strain AceU3 can further utilize acetate as an electron donor to continuously generate electricity.Conclusion: In the present study, we first rationally designed S. oneidensis for enhancing the electron generation by using acetate as sole carbon source and electron donor. Based on synthetic biology strategies, modular assembly of acetate metabolic pathways could be further extended to other exoelectrogens to improve the Coulombic efficiency and broaden the spectrum of available carbon sources in MFCs for bioelectricity production. |
| format |
article |
| author |
Junqi Zhang Junqi Zhang Zheng Chen Zheng Chen Changjiang Liu Changjiang Liu Jianxun Li Xingjuan An Xingjuan An Deguang Wu Xi Sun Baocai Zhang Baocai Zhang Longping Fu Feng Li Feng Li Hao Song Hao Song |
| author_facet |
Junqi Zhang Junqi Zhang Zheng Chen Zheng Chen Changjiang Liu Changjiang Liu Jianxun Li Xingjuan An Xingjuan An Deguang Wu Xi Sun Baocai Zhang Baocai Zhang Longping Fu Feng Li Feng Li Hao Song Hao Song |
| author_sort |
Junqi Zhang |
| title |
Construction of an Acetate Metabolic Pathway to Enhance Electron Generation of Engineered Shewanella oneidensis |
| title_short |
Construction of an Acetate Metabolic Pathway to Enhance Electron Generation of Engineered Shewanella oneidensis |
| title_full |
Construction of an Acetate Metabolic Pathway to Enhance Electron Generation of Engineered Shewanella oneidensis |
| title_fullStr |
Construction of an Acetate Metabolic Pathway to Enhance Electron Generation of Engineered Shewanella oneidensis |
| title_full_unstemmed |
Construction of an Acetate Metabolic Pathway to Enhance Electron Generation of Engineered Shewanella oneidensis |
| title_sort |
construction of an acetate metabolic pathway to enhance electron generation of engineered shewanella oneidensis |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/9a1a60fe777344fdafbe7ffd50820224 |
| work_keys_str_mv |
AT junqizhang constructionofanacetatemetabolicpathwaytoenhanceelectrongenerationofengineeredshewanellaoneidensis AT junqizhang constructionofanacetatemetabolicpathwaytoenhanceelectrongenerationofengineeredshewanellaoneidensis AT zhengchen constructionofanacetatemetabolicpathwaytoenhanceelectrongenerationofengineeredshewanellaoneidensis AT zhengchen constructionofanacetatemetabolicpathwaytoenhanceelectrongenerationofengineeredshewanellaoneidensis AT changjiangliu constructionofanacetatemetabolicpathwaytoenhanceelectrongenerationofengineeredshewanellaoneidensis AT changjiangliu constructionofanacetatemetabolicpathwaytoenhanceelectrongenerationofengineeredshewanellaoneidensis AT jianxunli constructionofanacetatemetabolicpathwaytoenhanceelectrongenerationofengineeredshewanellaoneidensis AT xingjuanan constructionofanacetatemetabolicpathwaytoenhanceelectrongenerationofengineeredshewanellaoneidensis AT xingjuanan constructionofanacetatemetabolicpathwaytoenhanceelectrongenerationofengineeredshewanellaoneidensis AT deguangwu constructionofanacetatemetabolicpathwaytoenhanceelectrongenerationofengineeredshewanellaoneidensis AT xisun constructionofanacetatemetabolicpathwaytoenhanceelectrongenerationofengineeredshewanellaoneidensis AT baocaizhang constructionofanacetatemetabolicpathwaytoenhanceelectrongenerationofengineeredshewanellaoneidensis AT baocaizhang constructionofanacetatemetabolicpathwaytoenhanceelectrongenerationofengineeredshewanellaoneidensis AT longpingfu constructionofanacetatemetabolicpathwaytoenhanceelectrongenerationofengineeredshewanellaoneidensis AT fengli constructionofanacetatemetabolicpathwaytoenhanceelectrongenerationofengineeredshewanellaoneidensis AT fengli constructionofanacetatemetabolicpathwaytoenhanceelectrongenerationofengineeredshewanellaoneidensis AT haosong constructionofanacetatemetabolicpathwaytoenhanceelectrongenerationofengineeredshewanellaoneidensis AT haosong constructionofanacetatemetabolicpathwaytoenhanceelectrongenerationofengineeredshewanellaoneidensis |
| _version_ |
1718420328828370944 |