Genetic adhesion profiles and adhesive variability of uropathogenic <i>Escherichia coli</i> strains

Our study was aimed at investigating prevalence of adhesion genetic determinants among uropathogenic E. coli strains and assessing their correlation with level of specific and non-specific adhesion. E. coli bacterial cultures (n = 33) isolated from patients with urinary tract infection were examined...

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Autores principales:	M. V. Kuznetsova, J. S. Gizatullina
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2021
Materias:	uropathogenic escherichia coli strains adhesins genes specific adhesion non-specific adhesion biofilm phylogroup Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/9a1ba4f30da44242b427295f1df4d8ac
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spelling	oai:doaj.org-article:9a1ba4f30da44242b427295f1df4d8ac2021-11-22T07:09:54ZGenetic adhesion profiles and adhesive variability of uropathogenic <i>Escherichia coli</i> strains2220-76192313-739810.15789/2220-7619-GAP-1413https://doaj.org/article/9a1ba4f30da44242b427295f1df4d8ac2021-06-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1413https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Our study was aimed at investigating prevalence of adhesion genetic determinants among uropathogenic E. coli strains and assessing their correlation with level of specific and non-specific adhesion. E. coli bacterial cultures (n = 33) isolated from patients with urinary tract infection were examined. A phylogenetic group of strains was detected by using Clermont quadriplex-PCR method. Detection of fimbrial and afimbrial adhesin genes was carried out with end-point PCR. Level of erythrocyte-specific, non-specific hydrophobic and hydrophilic adhesion as well as biofilm formation were estimated by using standard methods. Adhesin genes were detected with the following frequencies: fimH — 75.76%, flu — 66.67%, iha — 39.40%, papC — 33.33%. Each of the genes sfaDE, upaG, afa/draBC and yqi was found with frequency 18.18%, whereas eaeA was not detected. Seven strains (21.21%) carried solely fimbrial adhesin genes, three strains (9.09%) — afimbrial adhesin genes, and twenty-one strains (63.64%) had genes of both adhesin types. Twenty-three individual adhesion genotypes were found among thirty-three UPEC strains. A combination of at least four genes were detected in 45.45% strains, among which 60% belonged to phylogroup B2. Odds ratio for adhesin gene prevalence in B2 group was calculated. It was shown that in B2 group yqi and sfaDE genes were detected by 14-fold more frequently (OR = 14.286) than in other phylogroups, and flu gene was observed at 10-fold higher rate (OR = 10.000). No correlation between such genes and level of adhesion to erythrocytes was found, whereas fimH+, papC+ and upaG+ strains had higher level of non-specific hydrophilic adhesion. It was shown that fimbrial adhesins accounted for bacterial adhesion and biofilm formation stronger than afimbrial ones. Thicker biofilm tended to form on latex catheter surface for strains with positive genetic profile for adhesin gene carriers.M. V. KuznetsovaJ. S. GizatullinaSankt-Peterburg : NIIÈM imeni Pasteraarticleuropathogenic escherichia coli strainsadhesins genesspecific adhesionnon-specific adhesionbiofilmphylogroupInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 11, Iss 3, Pp 481-490 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	uropathogenic escherichia coli strains adhesins genes specific adhesion non-specific adhesion biofilm phylogroup Infectious and parasitic diseases RC109-216
spellingShingle	uropathogenic escherichia coli strains adhesins genes specific adhesion non-specific adhesion biofilm phylogroup Infectious and parasitic diseases RC109-216 M. V. Kuznetsova J. S. Gizatullina Genetic adhesion profiles and adhesive variability of uropathogenic <i>Escherichia coli</i> strains
description	Our study was aimed at investigating prevalence of adhesion genetic determinants among uropathogenic E. coli strains and assessing their correlation with level of specific and non-specific adhesion. E. coli bacterial cultures (n = 33) isolated from patients with urinary tract infection were examined. A phylogenetic group of strains was detected by using Clermont quadriplex-PCR method. Detection of fimbrial and afimbrial adhesin genes was carried out with end-point PCR. Level of erythrocyte-specific, non-specific hydrophobic and hydrophilic adhesion as well as biofilm formation were estimated by using standard methods. Adhesin genes were detected with the following frequencies: fimH — 75.76%, flu — 66.67%, iha — 39.40%, papC — 33.33%. Each of the genes sfaDE, upaG, afa/draBC and yqi was found with frequency 18.18%, whereas eaeA was not detected. Seven strains (21.21%) carried solely fimbrial adhesin genes, three strains (9.09%) — afimbrial adhesin genes, and twenty-one strains (63.64%) had genes of both adhesin types. Twenty-three individual adhesion genotypes were found among thirty-three UPEC strains. A combination of at least four genes were detected in 45.45% strains, among which 60% belonged to phylogroup B2. Odds ratio for adhesin gene prevalence in B2 group was calculated. It was shown that in B2 group yqi and sfaDE genes were detected by 14-fold more frequently (OR = 14.286) than in other phylogroups, and flu gene was observed at 10-fold higher rate (OR = 10.000). No correlation between such genes and level of adhesion to erythrocytes was found, whereas fimH+, papC+ and upaG+ strains had higher level of non-specific hydrophilic adhesion. It was shown that fimbrial adhesins accounted for bacterial adhesion and biofilm formation stronger than afimbrial ones. Thicker biofilm tended to form on latex catheter surface for strains with positive genetic profile for adhesin gene carriers.
format	article
author	M. V. Kuznetsova J. S. Gizatullina
author_facet	M. V. Kuznetsova J. S. Gizatullina
author_sort	M. V. Kuznetsova
title	Genetic adhesion profiles and adhesive variability of uropathogenic <i>Escherichia coli</i> strains
title_short	Genetic adhesion profiles and adhesive variability of uropathogenic <i>Escherichia coli</i> strains
title_full	Genetic adhesion profiles and adhesive variability of uropathogenic <i>Escherichia coli</i> strains
title_fullStr	Genetic adhesion profiles and adhesive variability of uropathogenic <i>Escherichia coli</i> strains
title_full_unstemmed	Genetic adhesion profiles and adhesive variability of uropathogenic <i>Escherichia coli</i> strains
title_sort	genetic adhesion profiles and adhesive variability of uropathogenic <i>escherichia coli</i> strains
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2021
url	https://doaj.org/article/9a1ba4f30da44242b427295f1df4d8ac
work_keys_str_mv	AT mvkuznetsova geneticadhesionprofilesandadhesivevariabilityofuropathogeniciescherichiacoliistrains AT jsgizatullina geneticadhesionprofilesandadhesivevariabilityofuropathogeniciescherichiacoliistrains
_version_	1718417932073041920

Genetic adhesion profiles and adhesive variability of uropathogenic <i>Escherichia coli</i> strains

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