Identification of extracellular glycerophosphodiesterases in Pseudomonas and their role in soil organic phosphorus remineralisation

Abstract In soils, phosphorus (P) exists in numerous organic and inorganic forms. However, plants can only acquire inorganic orthophosphate (Pi), meaning global crop production is frequently limited by P availability. To overcome this problem, rock phosphate fertilisers are heavily applied, often wi...

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Autores principales:	Ian D. E. A. Lidbury, Andrew R. J. Murphy, Tandra D. Fraser, Gary D. Bending, Alexandra M. E. Jones, Jonathan D. Moore, Andrew Goodall, Mark Tibbett, John P. Hammond, David J. Scanlan, Elizabeth M. H. Wellington
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9a2a1bc08e2a4e4d979823a81956a0d5
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spelling	oai:doaj.org-article:9a2a1bc08e2a4e4d979823a81956a0d52021-12-02T12:32:29ZIdentification of extracellular glycerophosphodiesterases in Pseudomonas and their role in soil organic phosphorus remineralisation10.1038/s41598-017-02327-62045-2322https://doaj.org/article/9a2a1bc08e2a4e4d979823a81956a0d52017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02327-6https://doaj.org/toc/2045-2322Abstract In soils, phosphorus (P) exists in numerous organic and inorganic forms. However, plants can only acquire inorganic orthophosphate (Pi), meaning global crop production is frequently limited by P availability. To overcome this problem, rock phosphate fertilisers are heavily applied, often with negative environmental and socio-economic consequences. The organic P fraction of soil contains phospholipids that are rapidly degraded resulting in the release of bioavailable Pi. However, the mechanisms behind this process remain unknown. We identified and experimentally confirmed the function of two secreted glycerolphosphodiesterases, GlpQI and GlpQII, found in Pseudomonas stutzeri DSM4166 and Pseudomonas fluorescens SBW25, respectively. A series of co-cultivation experiments revealed that in these Pseudomonas strains, cleavage of glycerolphosphorylcholine and its breakdown product G3P occurs extracellularly allowing other bacteria to benefit from this metabolism. Analyses of metagenomic and metatranscriptomic datasets revealed that this trait is widespread among soil bacteria with Actinobacteria and Proteobacteria, specifically Betaproteobacteria and Gammaproteobacteria, the likely major players.Ian D. E. A. LidburyAndrew R. J. MurphyTandra D. FraserGary D. BendingAlexandra M. E. JonesJonathan D. MooreAndrew GoodallMark TibbettJohn P. HammondDavid J. ScanlanElizabeth M. H. WellingtonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ian D. E. A. Lidbury Andrew R. J. Murphy Tandra D. Fraser Gary D. Bending Alexandra M. E. Jones Jonathan D. Moore Andrew Goodall Mark Tibbett John P. Hammond David J. Scanlan Elizabeth M. H. Wellington Identification of extracellular glycerophosphodiesterases in Pseudomonas and their role in soil organic phosphorus remineralisation
description	Abstract In soils, phosphorus (P) exists in numerous organic and inorganic forms. However, plants can only acquire inorganic orthophosphate (Pi), meaning global crop production is frequently limited by P availability. To overcome this problem, rock phosphate fertilisers are heavily applied, often with negative environmental and socio-economic consequences. The organic P fraction of soil contains phospholipids that are rapidly degraded resulting in the release of bioavailable Pi. However, the mechanisms behind this process remain unknown. We identified and experimentally confirmed the function of two secreted glycerolphosphodiesterases, GlpQI and GlpQII, found in Pseudomonas stutzeri DSM4166 and Pseudomonas fluorescens SBW25, respectively. A series of co-cultivation experiments revealed that in these Pseudomonas strains, cleavage of glycerolphosphorylcholine and its breakdown product G3P occurs extracellularly allowing other bacteria to benefit from this metabolism. Analyses of metagenomic and metatranscriptomic datasets revealed that this trait is widespread among soil bacteria with Actinobacteria and Proteobacteria, specifically Betaproteobacteria and Gammaproteobacteria, the likely major players.
format	article
author	Ian D. E. A. Lidbury Andrew R. J. Murphy Tandra D. Fraser Gary D. Bending Alexandra M. E. Jones Jonathan D. Moore Andrew Goodall Mark Tibbett John P. Hammond David J. Scanlan Elizabeth M. H. Wellington
author_facet	Ian D. E. A. Lidbury Andrew R. J. Murphy Tandra D. Fraser Gary D. Bending Alexandra M. E. Jones Jonathan D. Moore Andrew Goodall Mark Tibbett John P. Hammond David J. Scanlan Elizabeth M. H. Wellington
author_sort	Ian D. E. A. Lidbury
title	Identification of extracellular glycerophosphodiesterases in Pseudomonas and their role in soil organic phosphorus remineralisation
title_short	Identification of extracellular glycerophosphodiesterases in Pseudomonas and their role in soil organic phosphorus remineralisation
title_full	Identification of extracellular glycerophosphodiesterases in Pseudomonas and their role in soil organic phosphorus remineralisation
title_fullStr	Identification of extracellular glycerophosphodiesterases in Pseudomonas and their role in soil organic phosphorus remineralisation
title_full_unstemmed	Identification of extracellular glycerophosphodiesterases in Pseudomonas and their role in soil organic phosphorus remineralisation
title_sort	identification of extracellular glycerophosphodiesterases in pseudomonas and their role in soil organic phosphorus remineralisation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9a2a1bc08e2a4e4d979823a81956a0d5
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Identification of extracellular glycerophosphodiesterases in Pseudomonas and their role in soil organic phosphorus remineralisation

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