Effect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2α/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation

Abstract Tahiti lemon juice (Citrus latifolia) (TLJ), as a natural source of flavonoids, has been used as an alternative to anti-inflammatory drugs for the treatment of dysmenorrhea and menstrual excessive bleeding, often associated with an imbalance of the prostaglandins (PG) levels. However, despi...

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Autores principales:	Thaiane Robeldo, Edione Fatima Canzi, Priscila Maria de Andrade, Jhonne Pedro Pedotte Santana, Felipe Roberti Teixeira, Valentine Spagnol, Beatriz Helena Lameiro Noronha Sales Maia, Maristela Carbol, Erika Gonçalves Caneira, Maria Fátima das Graças Fernandes Da Silva, Ricardo Carneiro Borra
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9a2fd92dc41f4792bc182c1ec1c0205c
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spelling	oai:doaj.org-article:9a2fd92dc41f4792bc182c1ec1c0205c2021-12-02T17:14:59ZEffect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2α/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation10.1038/s41598-020-63477-82045-2322https://doaj.org/article/9a2fd92dc41f4792bc182c1ec1c0205c2020-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-63477-8https://doaj.org/toc/2045-2322Abstract Tahiti lemon juice (Citrus latifolia) (TLJ), as a natural source of flavonoids, has been used as an alternative to anti-inflammatory drugs for the treatment of dysmenorrhea and menstrual excessive bleeding, often associated with an imbalance of the prostaglandins (PG) levels. However, despite the positive effects, the mechanisms that rule menstruation control are still unknown. Therefore, the objectives were to characterize the TLJ and analyze its effect on the production of PGF2α, PGE2 and pro-inflammatory cytokines involved inmenstruation. Flavonoids from TLJ were discriminated by UPLC-DAD-MS/MS (Qq-TOF) and the effects of TLJ were studied in vitro by quantification of the contraction of myoblasts in culture and PGF2α and PGE2 productions. Further, the systemic and menstrual fluid levels of PGF2α, PGE2, IL-1β, TNF-α, IL-6, AK1B1 and AK1C3 enzymes produced by women during the menstrual period were compared after exposition or not to TLJ or meloxicam. The results showed that TLJ induces an increase in the contraction of myoblasts and the PGF2α supernatant level. Regarding in vivo analysis, a higher concentration of PGF2α and an unaltered PGE2 level was also found in the menstrual blood of women treated with TLJ, in contrast with a lower level of PGE2 and PGF2α observed in the meloxicam group. Concerning cytokines, only menstrual TNF-α levels decrease after treatment with TLJ or meloxicam. In conclusion, TLJ may favor the control of menstruation events via a PGF2α mediated muscle contractile response.Thaiane RobeldoEdione Fatima CanziPriscila Maria de AndradeJhonne Pedro Pedotte SantanaFelipe Roberti TeixeiraValentine SpagnolBeatriz Helena Lameiro Noronha Sales MaiaMaristela CarbolErika Gonçalves CaneiraMaria Fátima das Graças Fernandes Da SilvaRicardo Carneiro BorraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-16 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thaiane Robeldo Edione Fatima Canzi Priscila Maria de Andrade Jhonne Pedro Pedotte Santana Felipe Roberti Teixeira Valentine Spagnol Beatriz Helena Lameiro Noronha Sales Maia Maristela Carbol Erika Gonçalves Caneira Maria Fátima das Graças Fernandes Da Silva Ricardo Carneiro Borra Effect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2α/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation
description	Abstract Tahiti lemon juice (Citrus latifolia) (TLJ), as a natural source of flavonoids, has been used as an alternative to anti-inflammatory drugs for the treatment of dysmenorrhea and menstrual excessive bleeding, often associated with an imbalance of the prostaglandins (PG) levels. However, despite the positive effects, the mechanisms that rule menstruation control are still unknown. Therefore, the objectives were to characterize the TLJ and analyze its effect on the production of PGF2α, PGE2 and pro-inflammatory cytokines involved inmenstruation. Flavonoids from TLJ were discriminated by UPLC-DAD-MS/MS (Qq-TOF) and the effects of TLJ were studied in vitro by quantification of the contraction of myoblasts in culture and PGF2α and PGE2 productions. Further, the systemic and menstrual fluid levels of PGF2α, PGE2, IL-1β, TNF-α, IL-6, AK1B1 and AK1C3 enzymes produced by women during the menstrual period were compared after exposition or not to TLJ or meloxicam. The results showed that TLJ induces an increase in the contraction of myoblasts and the PGF2α supernatant level. Regarding in vivo analysis, a higher concentration of PGF2α and an unaltered PGE2 level was also found in the menstrual blood of women treated with TLJ, in contrast with a lower level of PGE2 and PGF2α observed in the meloxicam group. Concerning cytokines, only menstrual TNF-α levels decrease after treatment with TLJ or meloxicam. In conclusion, TLJ may favor the control of menstruation events via a PGF2α mediated muscle contractile response.
format	article
author	Thaiane Robeldo Edione Fatima Canzi Priscila Maria de Andrade Jhonne Pedro Pedotte Santana Felipe Roberti Teixeira Valentine Spagnol Beatriz Helena Lameiro Noronha Sales Maia Maristela Carbol Erika Gonçalves Caneira Maria Fátima das Graças Fernandes Da Silva Ricardo Carneiro Borra
author_facet	Thaiane Robeldo Edione Fatima Canzi Priscila Maria de Andrade Jhonne Pedro Pedotte Santana Felipe Roberti Teixeira Valentine Spagnol Beatriz Helena Lameiro Noronha Sales Maia Maristela Carbol Erika Gonçalves Caneira Maria Fátima das Graças Fernandes Da Silva Ricardo Carneiro Borra
author_sort	Thaiane Robeldo
title	Effect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2α/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation
title_short	Effect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2α/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation
title_full	Effect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2α/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation
title_fullStr	Effect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2α/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation
title_full_unstemmed	Effect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2α/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation
title_sort	effect of tahiti lime (citrus latifolia) juice on the production of the pgf2α/pge2 and pro-inflammatory cytokines involved in menstruation
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/9a2fd92dc41f4792bc182c1ec1c0205c
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Effect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2α/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation

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