Rapid green synthesis of silver nanoparticles from Chrysanthemum indicum L and its antibacterial and cytotoxic effects: an in vitro study

Selvaraj Arokiyaraj,1 Mariadhas Valan Arasu,2 Savariar Vincent,3 Nyayirukannaian Udaya Prakash,4 Seong Ho Choi,5 Young-Kyoon Oh,1 Ki Choon Choi,2 Kyoung Hoon Kim1,61Department of Animal Nutrition and Physiology, National Institute of Animal Science, Rural Development Administration, Suwon, Republic...

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Autores principales:	Arokiyaraj S, Arasu MV, Vincent S, Udaya Prakash NK, Choi SH, Oh YK, Choi KC, Kim KH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9a36d0dd74424419bee9c163b11035d2
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spelling	oai:doaj.org-article:9a36d0dd74424419bee9c163b11035d22021-12-02T05:10:25ZRapid green synthesis of silver nanoparticles from Chrysanthemum indicum L and its antibacterial and cytotoxic effects: an in vitro study1178-2013https://doaj.org/article/9a36d0dd74424419bee9c163b11035d22014-01-01T00:00:00Zhttp://www.dovepress.com/rapid-green-synthesis-of-silver-nanoparticles-from-chrysanthemum-indic-a15439https://doaj.org/toc/1178-2013 Selvaraj Arokiyaraj,1 Mariadhas Valan Arasu,2 Savariar Vincent,3 Nyayirukannaian Udaya Prakash,4 Seong Ho Choi,5 Young-Kyoon Oh,1 Ki Choon Choi,2 Kyoung Hoon Kim1,61Department of Animal Nutrition and Physiology, National Institute of Animal Science, Rural Development Administration, Suwon, Republic of Korea; 2Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration, Seonghwan-Eup, Cheonan-Si, Chungnam, Republic of Korea; 3Center for Environmental Research and Development, Loyola College, Chennai, India; 4Research and Development, Vel Tech Dr RR and Dr SR Technical University, Chennai, India; 5Department of Animal Science, Chungbuk National University, Chungbuk, Republic of Korea; 6Department of Animal Science, Seoul National University, Pyeongchang, Republic of KoreaAbstract: The present work reports a simple, cost-effective, and ecofriendly method for the synthesis of silver nanoparticles (AgNPs) using Chrysanthemum indicum and its antibacterial and cytotoxic effects. The formation of AgNPs was confirmed by color change, and it was further characterized by ultraviolet–visible spectroscopy (435 nm). The phytochemical screening of C. indicum revealed the presence of flavonoids, terpenoids, and glycosides, suggesting that these compounds act as reducing and stabilizing agents. The crystalline nature of the synthesized particles was confirmed by X-ray diffraction, as they exhibited face-centered cubic symmetry. The size and morphology of the particles were characterized by transmission electron microscopy, which showed spherical shapes and sizes that ranged between 37.71–71.99 nm. Energy-dispersive X-ray spectroscopy documented the presence of silver. The antimicrobial effect of the synthesized AgNPs revealed a significant effect against the bacteria Klebsiella pneumonia, Escherichia coli, and Pseudomonas aeruginosa. Additionally, cytotoxic assays showed no toxicity of AgNPs toward 3T3 mouse embryo fibroblast cells (25 µg/mL); hence, these particles were safe to use.Keywords: antibacterial activity, Chrysanthemum indicum, green synthesis, silver nanoparticle, cytotoxicArokiyaraj SArasu MVVincent SUdaya Prakash NKChoi SHOh YKChoi KCKim KHDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 379-388 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Arokiyaraj S Arasu MV Vincent S Udaya Prakash NK Choi SH Oh YK Choi KC Kim KH Rapid green synthesis of silver nanoparticles from Chrysanthemum indicum L and its antibacterial and cytotoxic effects: an in vitro study
description	Selvaraj Arokiyaraj,1 Mariadhas Valan Arasu,2 Savariar Vincent,3 Nyayirukannaian Udaya Prakash,4 Seong Ho Choi,5 Young-Kyoon Oh,1 Ki Choon Choi,2 Kyoung Hoon Kim1,61Department of Animal Nutrition and Physiology, National Institute of Animal Science, Rural Development Administration, Suwon, Republic of Korea; 2Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration, Seonghwan-Eup, Cheonan-Si, Chungnam, Republic of Korea; 3Center for Environmental Research and Development, Loyola College, Chennai, India; 4Research and Development, Vel Tech Dr RR and Dr SR Technical University, Chennai, India; 5Department of Animal Science, Chungbuk National University, Chungbuk, Republic of Korea; 6Department of Animal Science, Seoul National University, Pyeongchang, Republic of KoreaAbstract: The present work reports a simple, cost-effective, and ecofriendly method for the synthesis of silver nanoparticles (AgNPs) using Chrysanthemum indicum and its antibacterial and cytotoxic effects. The formation of AgNPs was confirmed by color change, and it was further characterized by ultraviolet–visible spectroscopy (435 nm). The phytochemical screening of C. indicum revealed the presence of flavonoids, terpenoids, and glycosides, suggesting that these compounds act as reducing and stabilizing agents. The crystalline nature of the synthesized particles was confirmed by X-ray diffraction, as they exhibited face-centered cubic symmetry. The size and morphology of the particles were characterized by transmission electron microscopy, which showed spherical shapes and sizes that ranged between 37.71–71.99 nm. Energy-dispersive X-ray spectroscopy documented the presence of silver. The antimicrobial effect of the synthesized AgNPs revealed a significant effect against the bacteria Klebsiella pneumonia, Escherichia coli, and Pseudomonas aeruginosa. Additionally, cytotoxic assays showed no toxicity of AgNPs toward 3T3 mouse embryo fibroblast cells (25 µg/mL); hence, these particles were safe to use.Keywords: antibacterial activity, Chrysanthemum indicum, green synthesis, silver nanoparticle, cytotoxic
format	article
author	Arokiyaraj S Arasu MV Vincent S Udaya Prakash NK Choi SH Oh YK Choi KC Kim KH
author_facet	Arokiyaraj S Arasu MV Vincent S Udaya Prakash NK Choi SH Oh YK Choi KC Kim KH
author_sort	Arokiyaraj S
title	Rapid green synthesis of silver nanoparticles from Chrysanthemum indicum L and its antibacterial and cytotoxic effects: an in vitro study
title_short	Rapid green synthesis of silver nanoparticles from Chrysanthemum indicum L and its antibacterial and cytotoxic effects: an in vitro study
title_full	Rapid green synthesis of silver nanoparticles from Chrysanthemum indicum L and its antibacterial and cytotoxic effects: an in vitro study
title_fullStr	Rapid green synthesis of silver nanoparticles from Chrysanthemum indicum L and its antibacterial and cytotoxic effects: an in vitro study
title_full_unstemmed	Rapid green synthesis of silver nanoparticles from Chrysanthemum indicum L and its antibacterial and cytotoxic effects: an in vitro study
title_sort	rapid green synthesis of silver nanoparticles from chrysanthemum indicum l and its antibacterial and cytotoxic effects: an in vitro study
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/9a36d0dd74424419bee9c163b11035d2
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Rapid green synthesis of silver nanoparticles from Chrysanthemum indicum L and its antibacterial and cytotoxic effects: an in vitro study

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