Scalable Culturing of Primary Human Glioblastoma Tumor-Initiating Cells with a Cell-Friendly Culture System

Abstract Glioblastoma is the most aggressive and deadly brain cancer. There is growing interest to develop drugs that specifically target to glioblastoma tumor-initiating cells (TICs). However, the cost-effective production of large numbers of high quality glioblastoma TICs for drug discovery with c...

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Autores principales:	Qiang Li, Haishuang Lin, Jack Rauch, Loic P. Deleyrolle, Brent A. Reynolds, Hendrik J. Viljoen, Chi Zhang, Linxia Gu, Erika Van Wyk, Yuguo Lei
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9a37707ea8514ad2b6d1c3bb740e5907
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spelling	oai:doaj.org-article:9a37707ea8514ad2b6d1c3bb740e59072021-12-02T11:41:14ZScalable Culturing of Primary Human Glioblastoma Tumor-Initiating Cells with a Cell-Friendly Culture System10.1038/s41598-018-21927-42045-2322https://doaj.org/article/9a37707ea8514ad2b6d1c3bb740e59072018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21927-4https://doaj.org/toc/2045-2322Abstract Glioblastoma is the most aggressive and deadly brain cancer. There is growing interest to develop drugs that specifically target to glioblastoma tumor-initiating cells (TICs). However, the cost-effective production of large numbers of high quality glioblastoma TICs for drug discovery with current cell culturing technologies remains very challenging. Here, we report a new method that cultures glioblastoma TICs in microscale alginate hydrogel tubes (or AlgTubes). The AlgTubes allowed long-term culturing (~50 days, 10 passages) of glioblastoma TICs with high growth rate (~700-fold expansion/14 days), high cell viability and high volumetric yield (~3.0 × 108 cells/mL) without losing the stem cell properties, all offered large advancements over current culturing methods. This method can be applied for the scalable production of glioblastoma TICs at affordable cost for drug discovery.Qiang LiHaishuang LinJack RauchLoic P. DeleyrolleBrent A. ReynoldsHendrik J. ViljoenChi ZhangChi ZhangLinxia GuErika Van WykYuguo LeiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qiang Li Haishuang Lin Jack Rauch Loic P. Deleyrolle Brent A. Reynolds Hendrik J. Viljoen Chi Zhang Chi Zhang Linxia Gu Erika Van Wyk Yuguo Lei Scalable Culturing of Primary Human Glioblastoma Tumor-Initiating Cells with a Cell-Friendly Culture System
description	Abstract Glioblastoma is the most aggressive and deadly brain cancer. There is growing interest to develop drugs that specifically target to glioblastoma tumor-initiating cells (TICs). However, the cost-effective production of large numbers of high quality glioblastoma TICs for drug discovery with current cell culturing technologies remains very challenging. Here, we report a new method that cultures glioblastoma TICs in microscale alginate hydrogel tubes (or AlgTubes). The AlgTubes allowed long-term culturing (~50 days, 10 passages) of glioblastoma TICs with high growth rate (~700-fold expansion/14 days), high cell viability and high volumetric yield (~3.0 × 108 cells/mL) without losing the stem cell properties, all offered large advancements over current culturing methods. This method can be applied for the scalable production of glioblastoma TICs at affordable cost for drug discovery.
format	article
author	Qiang Li Haishuang Lin Jack Rauch Loic P. Deleyrolle Brent A. Reynolds Hendrik J. Viljoen Chi Zhang Chi Zhang Linxia Gu Erika Van Wyk Yuguo Lei
author_facet	Qiang Li Haishuang Lin Jack Rauch Loic P. Deleyrolle Brent A. Reynolds Hendrik J. Viljoen Chi Zhang Chi Zhang Linxia Gu Erika Van Wyk Yuguo Lei
author_sort	Qiang Li
title	Scalable Culturing of Primary Human Glioblastoma Tumor-Initiating Cells with a Cell-Friendly Culture System
title_short	Scalable Culturing of Primary Human Glioblastoma Tumor-Initiating Cells with a Cell-Friendly Culture System
title_full	Scalable Culturing of Primary Human Glioblastoma Tumor-Initiating Cells with a Cell-Friendly Culture System
title_fullStr	Scalable Culturing of Primary Human Glioblastoma Tumor-Initiating Cells with a Cell-Friendly Culture System
title_full_unstemmed	Scalable Culturing of Primary Human Glioblastoma Tumor-Initiating Cells with a Cell-Friendly Culture System
title_sort	scalable culturing of primary human glioblastoma tumor-initiating cells with a cell-friendly culture system
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/9a37707ea8514ad2b6d1c3bb740e5907
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Scalable Culturing of Primary Human Glioblastoma Tumor-Initiating Cells with a Cell-Friendly Culture System

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