A DEFICIENCY OF ANTIGEN-PRESENTING CELLS IN PATIENTS WITH PULMONARY TUBERCULOSIS

Abstract. The phenotype and functional properties of antigen-presenting cells (APCs: blood monocytes and in vitro generated macrophages/dendritic cells) were investigated in patients with pulmonary tuberculosis (TB, n = 192) with different levels of proliferative response to M. tuberculosis antigens...

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Autores principales:	L. V. Sakhno, Zh. M. Raspay, M. A. Tikhonova, S. D. Niconov, O. A. Zhdanov, A. A. Ostanin, E. R. Chernykh
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	monocytes macrophages dendritic cellsи cytokines pulmonary tuberculosis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/9a38ace2e78644b48d34fc7852cc8e93
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spelling	oai:doaj.org-article:9a38ace2e78644b48d34fc7852cc8e932021-11-18T08:03:40ZA DEFICIENCY OF ANTIGEN-PRESENTING CELLS IN PATIENTS WITH PULMONARY TUBERCULOSIS1563-06252313-741X10.15789/1563-0625-2009-2-3-245-254https://doaj.org/article/9a38ace2e78644b48d34fc7852cc8e932014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/311https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. The phenotype and functional properties of antigen-presenting cells (APCs: blood monocytes and in vitro generated macrophages/dendritic cells) were investigated in patients with pulmonary tuberculosis (TB, n = 192) with different levels of proliferative response to M. tuberculosis antigens (PPD-responsive vs PPD - anergic patients, n = 118 and 74, respectively). A functional deficiency of all 3 types of APCs was revealed in patients with TB. I.e., a monocyte disfunction was displayed by low CD86 and HLA-DR expression, 2-fold increase of CD14+CD16+ subset, high level of FasL+ and IL-10+ cells, and enhanced IL-10 and IL-6 production upon LPS-stimulation. The in vitro generated macrophages from blood monocytes challenged with GM-CSF, were characterized by shifted Th1/Th2 balance (down-regulated production of IFNγ and IL-18 combined with up-regulation of IL-6 and IL-10), and reduced allostimulatory activity in mixed lymphocyte culture. The dendritic cells were characterized by decrease of mature, activated CD25+ cells, low level of IFNγ production in conjunction with enhanced capacity to produce IL - 10 and IL-6, and profound reduction of functional (allostimulatory) activity. The APC disfunction of were most prominent in PPD-anergic patients. A possible role of APC disfunctions in disturbed antigen-specific T-cell response to M. tuberculosis is discussed.L. V. SakhnoZh. M. RaspayM. A. TikhonovaS. D. NiconovO. A. ZhdanovA. A. OstaninE. R. ChernykhSPb RAACIarticlemonocytesmacrophagesdendritic cellsиcytokinespulmonary tuberculosisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 11, Iss 2-3, Pp 245-254 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	monocytes macrophages dendritic cellsи cytokines pulmonary tuberculosis Immunologic diseases. Allergy RC581-607
spellingShingle	monocytes macrophages dendritic cellsи cytokines pulmonary tuberculosis Immunologic diseases. Allergy RC581-607 L. V. Sakhno Zh. M. Raspay M. A. Tikhonova S. D. Niconov O. A. Zhdanov A. A. Ostanin E. R. Chernykh A DEFICIENCY OF ANTIGEN-PRESENTING CELLS IN PATIENTS WITH PULMONARY TUBERCULOSIS
description	Abstract. The phenotype and functional properties of antigen-presenting cells (APCs: blood monocytes and in vitro generated macrophages/dendritic cells) were investigated in patients with pulmonary tuberculosis (TB, n = 192) with different levels of proliferative response to M. tuberculosis antigens (PPD-responsive vs PPD - anergic patients, n = 118 and 74, respectively). A functional deficiency of all 3 types of APCs was revealed in patients with TB. I.e., a monocyte disfunction was displayed by low CD86 and HLA-DR expression, 2-fold increase of CD14+CD16+ subset, high level of FasL+ and IL-10+ cells, and enhanced IL-10 and IL-6 production upon LPS-stimulation. The in vitro generated macrophages from blood monocytes challenged with GM-CSF, were characterized by shifted Th1/Th2 balance (down-regulated production of IFNγ and IL-18 combined with up-regulation of IL-6 and IL-10), and reduced allostimulatory activity in mixed lymphocyte culture. The dendritic cells were characterized by decrease of mature, activated CD25+ cells, low level of IFNγ production in conjunction with enhanced capacity to produce IL - 10 and IL-6, and profound reduction of functional (allostimulatory) activity. The APC disfunction of were most prominent in PPD-anergic patients. A possible role of APC disfunctions in disturbed antigen-specific T-cell response to M. tuberculosis is discussed.
format	article
author	L. V. Sakhno Zh. M. Raspay M. A. Tikhonova S. D. Niconov O. A. Zhdanov A. A. Ostanin E. R. Chernykh
author_facet	L. V. Sakhno Zh. M. Raspay M. A. Tikhonova S. D. Niconov O. A. Zhdanov A. A. Ostanin E. R. Chernykh
author_sort	L. V. Sakhno
title	A DEFICIENCY OF ANTIGEN-PRESENTING CELLS IN PATIENTS WITH PULMONARY TUBERCULOSIS
title_short	A DEFICIENCY OF ANTIGEN-PRESENTING CELLS IN PATIENTS WITH PULMONARY TUBERCULOSIS
title_full	A DEFICIENCY OF ANTIGEN-PRESENTING CELLS IN PATIENTS WITH PULMONARY TUBERCULOSIS
title_fullStr	A DEFICIENCY OF ANTIGEN-PRESENTING CELLS IN PATIENTS WITH PULMONARY TUBERCULOSIS
title_full_unstemmed	A DEFICIENCY OF ANTIGEN-PRESENTING CELLS IN PATIENTS WITH PULMONARY TUBERCULOSIS
title_sort	deficiency of antigen-presenting cells in patients with pulmonary tuberculosis
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/9a38ace2e78644b48d34fc7852cc8e93
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A DEFICIENCY OF ANTIGEN-PRESENTING CELLS IN PATIENTS WITH PULMONARY TUBERCULOSIS

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