Investigation of immunogenic properties of Hemolin from silkworm, Bombyx mori as carrier protein: an immunoinformatic approach

Abstract Infectious diseases are the major cause of high mortality among infants and geriatric patients. Vaccines are the only weapon in our arsenal to defend us ourselves against innumerable infectious diseases. Though myriad of vaccines are available, still countless people die due to microbial in...

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Autores principales:	Veeranarayanan Surya Aathmanathan, Nattarsingam Jothi, Vijay Kumar Prajapati, Muthukalingan Krishnan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9a3bf50bb4a348e7903e148a6de8d539
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spelling	oai:doaj.org-article:9a3bf50bb4a348e7903e148a6de8d5392021-12-02T11:40:53ZInvestigation of immunogenic properties of Hemolin from silkworm, Bombyx mori as carrier protein: an immunoinformatic approach10.1038/s41598-018-25374-z2045-2322https://doaj.org/article/9a3bf50bb4a348e7903e148a6de8d5392018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25374-zhttps://doaj.org/toc/2045-2322Abstract Infectious diseases are the major cause of high mortality among infants and geriatric patients. Vaccines are the only weapon in our arsenal to defend us ourselves against innumerable infectious diseases. Though myriad of vaccines are available, still countless people die due to microbial infections. Subunit vaccine is an effective strategy of vaccine development, combining a highly immunogenic carrier protein with highly antigenic but non–immunogenic antigen (haptens). In this study we have made an attempt to utilize the immunoinformatic tool for carrier protein development. Immunogenic mediators (T-cell, B-cell, IFN-γ epitopes) and physiochemical properties of hemolin protein of silkworm, Bombyx mori were studied. Hemolin was found to be non-allergic and highly antigenic in nature. The refined tertiary structure of modelled hemolin was docked against TLR3 and TLR4-MD2 complex. Molecular dynamics study emphasized the stable microscopic interaction between hemolin and TLRs. In-silico cloning and codon optimization was carried out for effective expression of hemolin in E. coli expression system. The overall presence of Cytotoxic T Lymphocytes (CTL), Humoral T Lymphocytes (HTL), and IFN-γ epitopes with high antigenicity depicts the potential of hemolin as a good candidate for carrier protein.Veeranarayanan Surya AathmanathanNattarsingam JothiVijay Kumar PrajapatiMuthukalingan KrishnanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Veeranarayanan Surya Aathmanathan Nattarsingam Jothi Vijay Kumar Prajapati Muthukalingan Krishnan Investigation of immunogenic properties of Hemolin from silkworm, Bombyx mori as carrier protein: an immunoinformatic approach
description	Abstract Infectious diseases are the major cause of high mortality among infants and geriatric patients. Vaccines are the only weapon in our arsenal to defend us ourselves against innumerable infectious diseases. Though myriad of vaccines are available, still countless people die due to microbial infections. Subunit vaccine is an effective strategy of vaccine development, combining a highly immunogenic carrier protein with highly antigenic but non–immunogenic antigen (haptens). In this study we have made an attempt to utilize the immunoinformatic tool for carrier protein development. Immunogenic mediators (T-cell, B-cell, IFN-γ epitopes) and physiochemical properties of hemolin protein of silkworm, Bombyx mori were studied. Hemolin was found to be non-allergic and highly antigenic in nature. The refined tertiary structure of modelled hemolin was docked against TLR3 and TLR4-MD2 complex. Molecular dynamics study emphasized the stable microscopic interaction between hemolin and TLRs. In-silico cloning and codon optimization was carried out for effective expression of hemolin in E. coli expression system. The overall presence of Cytotoxic T Lymphocytes (CTL), Humoral T Lymphocytes (HTL), and IFN-γ epitopes with high antigenicity depicts the potential of hemolin as a good candidate for carrier protein.
format	article
author	Veeranarayanan Surya Aathmanathan Nattarsingam Jothi Vijay Kumar Prajapati Muthukalingan Krishnan
author_facet	Veeranarayanan Surya Aathmanathan Nattarsingam Jothi Vijay Kumar Prajapati Muthukalingan Krishnan
author_sort	Veeranarayanan Surya Aathmanathan
title	Investigation of immunogenic properties of Hemolin from silkworm, Bombyx mori as carrier protein: an immunoinformatic approach
title_short	Investigation of immunogenic properties of Hemolin from silkworm, Bombyx mori as carrier protein: an immunoinformatic approach
title_full	Investigation of immunogenic properties of Hemolin from silkworm, Bombyx mori as carrier protein: an immunoinformatic approach
title_fullStr	Investigation of immunogenic properties of Hemolin from silkworm, Bombyx mori as carrier protein: an immunoinformatic approach
title_full_unstemmed	Investigation of immunogenic properties of Hemolin from silkworm, Bombyx mori as carrier protein: an immunoinformatic approach
title_sort	investigation of immunogenic properties of hemolin from silkworm, bombyx mori as carrier protein: an immunoinformatic approach
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/9a3bf50bb4a348e7903e148a6de8d539
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Investigation of immunogenic properties of Hemolin from silkworm, Bombyx mori as carrier protein: an immunoinformatic approach

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