LIF-free embryonic stem cell culture in simulated microgravity.
<h4>Background</h4>Leukemia inhibitory factor (LIF) is an indispensable factor for maintaining mouse embryonic stem (ES) cell pluripotency. A feeder layer and serum are also needed to maintain an undifferentiated state, however, such animal derived materials need to be eliminated for cli...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2009
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/9a3c9e03e57448ccaafe52842fa62eb8 |
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| Sumario: | <h4>Background</h4>Leukemia inhibitory factor (LIF) is an indispensable factor for maintaining mouse embryonic stem (ES) cell pluripotency. A feeder layer and serum are also needed to maintain an undifferentiated state, however, such animal derived materials need to be eliminated for clinical applications. Therefore, a more reliable ES cell culture technique is required.<h4>Methodology/principal findings</h4>We cultured mouse ES cells in simulated microgravity using a 3D-clinostat. We used feeder-free and serum-free media without LIF.<h4>Conclusions/significance</h4>Here we show that simulated microgravity allows novel LIF-free and animal derived material-free culture methods for mouse ES cells. |
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