LIF-free embryonic stem cell culture in simulated microgravity.

<h4>Background</h4>Leukemia inhibitory factor (LIF) is an indispensable factor for maintaining mouse embryonic stem (ES) cell pluripotency. A feeder layer and serum are also needed to maintain an undifferentiated state, however, such animal derived materials need to be eliminated for cli...

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Autores principales:	Yumi Kawahara, Tomotaka Manabe, Masaya Matsumoto, Teruyuki Kajiume, Masayasu Matsumoto, Louis Yuge
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9a3c9e03e57448ccaafe52842fa62eb8
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spelling	oai:doaj.org-article:9a3c9e03e57448ccaafe52842fa62eb82021-11-25T06:21:27ZLIF-free embryonic stem cell culture in simulated microgravity.1932-620310.1371/journal.pone.0006343https://doaj.org/article/9a3c9e03e57448ccaafe52842fa62eb82009-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19626124/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Leukemia inhibitory factor (LIF) is an indispensable factor for maintaining mouse embryonic stem (ES) cell pluripotency. A feeder layer and serum are also needed to maintain an undifferentiated state, however, such animal derived materials need to be eliminated for clinical applications. Therefore, a more reliable ES cell culture technique is required.<h4>Methodology/principal findings</h4>We cultured mouse ES cells in simulated microgravity using a 3D-clinostat. We used feeder-free and serum-free media without LIF.<h4>Conclusions/significance</h4>Here we show that simulated microgravity allows novel LIF-free and animal derived material-free culture methods for mouse ES cells.Yumi KawaharaTomotaka ManabeMasaya MatsumotoTeruyuki KajiumeMasayasu MatsumotoLouis YugePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 7, p e6343 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yumi Kawahara Tomotaka Manabe Masaya Matsumoto Teruyuki Kajiume Masayasu Matsumoto Louis Yuge LIF-free embryonic stem cell culture in simulated microgravity.
description	<h4>Background</h4>Leukemia inhibitory factor (LIF) is an indispensable factor for maintaining mouse embryonic stem (ES) cell pluripotency. A feeder layer and serum are also needed to maintain an undifferentiated state, however, such animal derived materials need to be eliminated for clinical applications. Therefore, a more reliable ES cell culture technique is required.<h4>Methodology/principal findings</h4>We cultured mouse ES cells in simulated microgravity using a 3D-clinostat. We used feeder-free and serum-free media without LIF.<h4>Conclusions/significance</h4>Here we show that simulated microgravity allows novel LIF-free and animal derived material-free culture methods for mouse ES cells.
format	article
author	Yumi Kawahara Tomotaka Manabe Masaya Matsumoto Teruyuki Kajiume Masayasu Matsumoto Louis Yuge
author_facet	Yumi Kawahara Tomotaka Manabe Masaya Matsumoto Teruyuki Kajiume Masayasu Matsumoto Louis Yuge
author_sort	Yumi Kawahara
title	LIF-free embryonic stem cell culture in simulated microgravity.
title_short	LIF-free embryonic stem cell culture in simulated microgravity.
title_full	LIF-free embryonic stem cell culture in simulated microgravity.
title_fullStr	LIF-free embryonic stem cell culture in simulated microgravity.
title_full_unstemmed	LIF-free embryonic stem cell culture in simulated microgravity.
title_sort	lif-free embryonic stem cell culture in simulated microgravity.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/9a3c9e03e57448ccaafe52842fa62eb8
work_keys_str_mv	AT yumikawahara liffreeembryonicstemcellcultureinsimulatedmicrogravity AT tomotakamanabe liffreeembryonicstemcellcultureinsimulatedmicrogravity AT masayamatsumoto liffreeembryonicstemcellcultureinsimulatedmicrogravity AT teruyukikajiume liffreeembryonicstemcellcultureinsimulatedmicrogravity AT masayasumatsumoto liffreeembryonicstemcellcultureinsimulatedmicrogravity AT louisyuge liffreeembryonicstemcellcultureinsimulatedmicrogravity
_version_	1718413815437066240

LIF-free embryonic stem cell culture in simulated microgravity.

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