Optimization of culture conditions by response surface methodology for production of extracellular esterase from Serratia sp. EST-4

Objective: The aim of present study was to determine the effect of interaction among different production variables on esterase production from Serratia sp. EST-4. Method: The optimum and combined effect of independent parameters were determined using Plackett-Burman and Central Composite Design (CC...

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Autores principales:	Kamal Kumar Bhardwaj, Rakesh Kumar, Suhani Bhagta, Reena Gupta
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Response surface methodology Esterase Serratia sp. Central composite design Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/9a459a1b75e94b4c94d9251bb1543b2b
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spelling	oai:doaj.org-article:9a459a1b75e94b4c94d9251bb1543b2b2021-11-18T04:43:58ZOptimization of culture conditions by response surface methodology for production of extracellular esterase from Serratia sp. EST-41018-364710.1016/j.jksus.2021.101603https://doaj.org/article/9a459a1b75e94b4c94d9251bb1543b2b2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1018364721002652https://doaj.org/toc/1018-3647Objective: The aim of present study was to determine the effect of interaction among different production variables on esterase production from Serratia sp. EST-4. Method: The optimum and combined effect of independent parameters were determined using Plackett-Burman and Central Composite Design (CCD) (Design Expert 11). Results: The effect of six parameters-cotton seed oil, peptone and maltose concentrations, pH, temperature and inoculum volume for production of esterase was determined by Plackett-Burman design. Four factors i.e. inoculum size, pH, peptone concentration and cotton seed oil concentration had significant effect on yield of esterase as suggested by the Pareto chart. Under optimized conditions, (inoculum size (1.0%, v/v), pH (8.0), peptone concentration (1.5%, w/v) and cotton seed oil concentration (4.0%, v/v)), the maximum esterase production was 9.77 U/ml. Analysis of variance (ANOVA) of the Central Composite Design (CCD)-based experiment exhibited the model F-value of 44.24 and P-value of <0.0001 which implies that model was significant and adequate to represent the system. Conclusion: Inoculum size 1.0%, v/v, pH 8.0, concentration of peptone 1.5%, w/v and concentration of cotton seed oil 4.0%, v/v had positive effect on esterase production and provided 1.52-fold increase in esterase production than that obtained by optimizing one factor at one time.Kamal Kumar BhardwajRakesh KumarSuhani BhagtaReena GuptaElsevierarticleResponse surface methodologyEsteraseSerratia sp.Central composite designScience (General)Q1-390ENJournal of King Saud University: Science, Vol 33, Iss 8, Pp 101603- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Response surface methodology Esterase Serratia sp. Central composite design Science (General) Q1-390
spellingShingle	Response surface methodology Esterase Serratia sp. Central composite design Science (General) Q1-390 Kamal Kumar Bhardwaj Rakesh Kumar Suhani Bhagta Reena Gupta Optimization of culture conditions by response surface methodology for production of extracellular esterase from Serratia sp. EST-4
description	Objective: The aim of present study was to determine the effect of interaction among different production variables on esterase production from Serratia sp. EST-4. Method: The optimum and combined effect of independent parameters were determined using Plackett-Burman and Central Composite Design (CCD) (Design Expert 11). Results: The effect of six parameters-cotton seed oil, peptone and maltose concentrations, pH, temperature and inoculum volume for production of esterase was determined by Plackett-Burman design. Four factors i.e. inoculum size, pH, peptone concentration and cotton seed oil concentration had significant effect on yield of esterase as suggested by the Pareto chart. Under optimized conditions, (inoculum size (1.0%, v/v), pH (8.0), peptone concentration (1.5%, w/v) and cotton seed oil concentration (4.0%, v/v)), the maximum esterase production was 9.77 U/ml. Analysis of variance (ANOVA) of the Central Composite Design (CCD)-based experiment exhibited the model F-value of 44.24 and P-value of <0.0001 which implies that model was significant and adequate to represent the system. Conclusion: Inoculum size 1.0%, v/v, pH 8.0, concentration of peptone 1.5%, w/v and concentration of cotton seed oil 4.0%, v/v had positive effect on esterase production and provided 1.52-fold increase in esterase production than that obtained by optimizing one factor at one time.
format	article
author	Kamal Kumar Bhardwaj Rakesh Kumar Suhani Bhagta Reena Gupta
author_facet	Kamal Kumar Bhardwaj Rakesh Kumar Suhani Bhagta Reena Gupta
author_sort	Kamal Kumar Bhardwaj
title	Optimization of culture conditions by response surface methodology for production of extracellular esterase from Serratia sp. EST-4
title_short	Optimization of culture conditions by response surface methodology for production of extracellular esterase from Serratia sp. EST-4
title_full	Optimization of culture conditions by response surface methodology for production of extracellular esterase from Serratia sp. EST-4
title_fullStr	Optimization of culture conditions by response surface methodology for production of extracellular esterase from Serratia sp. EST-4
title_full_unstemmed	Optimization of culture conditions by response surface methodology for production of extracellular esterase from Serratia sp. EST-4
title_sort	optimization of culture conditions by response surface methodology for production of extracellular esterase from serratia sp. est-4
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/9a459a1b75e94b4c94d9251bb1543b2b
work_keys_str_mv	AT kamalkumarbhardwaj optimizationofcultureconditionsbyresponsesurfacemethodologyforproductionofextracellularesterasefromserratiaspest4 AT rakeshkumar optimizationofcultureconditionsbyresponsesurfacemethodologyforproductionofextracellularesterasefromserratiaspest4 AT suhanibhagta optimizationofcultureconditionsbyresponsesurfacemethodologyforproductionofextracellularesterasefromserratiaspest4 AT reenagupta optimizationofcultureconditionsbyresponsesurfacemethodologyforproductionofextracellularesterasefromserratiaspest4
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Optimization of culture conditions by response surface methodology for production of extracellular esterase from Serratia sp. EST-4

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