QUANTITATIVE EVALUATION OF IMMUNOCYTES AND INTERLEUKIN-17 LEVELS IN SALIVA FROM SMOKERS AT THE EARLY STAGES OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE

The study was focused on identification of disorders in immune homeostasis in the mucosalivary area, using cytofluorometric analysis of immune cell populations and quantitative enzyme immunoassay for interleukin-17 in saliva of radiochemical facility workers with a history of smoking, being at initi...

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Autores principales:	E. I. Altynbaeva, S. N. Teplova, S. A. Kochengina
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	chronic obstructive pulmonary disease (copd) salivary immunocytesa flow cytometry il-17 t helper cells Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/9a460b44d84244b4900d53ecaa1aa2db
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spelling	oai:doaj.org-article:9a460b44d84244b4900d53ecaa1aa2db2021-11-18T08:03:38ZQUANTITATIVE EVALUATION OF IMMUNOCYTES AND INTERLEUKIN-17 LEVELS IN SALIVA FROM SMOKERS AT THE EARLY STAGES OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE1563-06252313-741X10.15789/1563-0625-2010-4-5-399-404https://doaj.org/article/9a460b44d84244b4900d53ecaa1aa2db2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/217https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe study was focused on identification of disorders in immune homeostasis in the mucosalivary area, using cytofluorometric analysis of immune cell populations and quantitative enzyme immunoassay for interleukin-17 in saliva of radiochemical facility workers with a history of smoking, being at initial stages of chronic obstructive pulmonary diseases (COPD) without exacerbations. We have observed a cohort of COPD patients (144 workers), as well as a group of 264 smoking individuals without any signs of COPD. The study and control groups have been matched by age, gender, working conditions, smoking index and clinical history. During the last ten years, the persons under study were subject to a regular follow-up. The current study included twenty-three patients from the main group and ten individuals from the group of comparison. Analysis of immunocytes in saliva was peformed by means of flow cytometry. The patients for laboratory studies were selected in a random manner. Total amounts of leukocytes was measured in saliva, providing a mean value of 2.4106/ml, followed by filtration of saliva through porous filters (Becton Dickinson). Cytofluorimetric analysis was performed be means of BD FACSCanto II machine, using a kit of appropriate monoclonal antibodies, thus allowing of a four-colour fluorescence analysis. The salivary immune cell subpopulations were scored with viable cells, positive for CD4+. A significant increase in amounts of CD+CD8- (25.85% versus 1.4% in the control group, p = 0.049) and in CD+CD+ (3.3% versus 0.6%, p = 0.049) was noted in COPD patients, hence presuming an increase in total amounts of T-lymphocytes and T-helpers, without any enhancement of cytotoxic cell populations in the mucosalivary region, being permanently exposed to tobacco smoke in the smokers with COPD. The obtained findings let us to assume an involvement of CD+CD+ lymphocytes in pathogenesis of inflammatory alterations at COPD. An increased level of IL-17 was revealed in COPD patients group, versus those smokers, who had no clinical signs of COPD, thus suggesting activation of secretory functions in a subpopulation of CD+CD+ lymphocytes (i.e., Th17).E. I. AltynbaevaS. N. TeplovaS. A. KochenginaSPb RAACIarticlechronic obstructive pulmonary disease (copd)salivary immunocytesaflow cytometryil-17t helper cellsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 12, Iss 4-5, Pp 399-404 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	chronic obstructive pulmonary disease (copd) salivary immunocytesa flow cytometry il-17 t helper cells Immunologic diseases. Allergy RC581-607
spellingShingle	chronic obstructive pulmonary disease (copd) salivary immunocytesa flow cytometry il-17 t helper cells Immunologic diseases. Allergy RC581-607 E. I. Altynbaeva S. N. Teplova S. A. Kochengina QUANTITATIVE EVALUATION OF IMMUNOCYTES AND INTERLEUKIN-17 LEVELS IN SALIVA FROM SMOKERS AT THE EARLY STAGES OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE
description	The study was focused on identification of disorders in immune homeostasis in the mucosalivary area, using cytofluorometric analysis of immune cell populations and quantitative enzyme immunoassay for interleukin-17 in saliva of radiochemical facility workers with a history of smoking, being at initial stages of chronic obstructive pulmonary diseases (COPD) without exacerbations. We have observed a cohort of COPD patients (144 workers), as well as a group of 264 smoking individuals without any signs of COPD. The study and control groups have been matched by age, gender, working conditions, smoking index and clinical history. During the last ten years, the persons under study were subject to a regular follow-up. The current study included twenty-three patients from the main group and ten individuals from the group of comparison. Analysis of immunocytes in saliva was peformed by means of flow cytometry. The patients for laboratory studies were selected in a random manner. Total amounts of leukocytes was measured in saliva, providing a mean value of 2.4106/ml, followed by filtration of saliva through porous filters (Becton Dickinson). Cytofluorimetric analysis was performed be means of BD FACSCanto II machine, using a kit of appropriate monoclonal antibodies, thus allowing of a four-colour fluorescence analysis. The salivary immune cell subpopulations were scored with viable cells, positive for CD4+. A significant increase in amounts of CD+CD8- (25.85% versus 1.4% in the control group, p = 0.049) and in CD+CD+ (3.3% versus 0.6%, p = 0.049) was noted in COPD patients, hence presuming an increase in total amounts of T-lymphocytes and T-helpers, without any enhancement of cytotoxic cell populations in the mucosalivary region, being permanently exposed to tobacco smoke in the smokers with COPD. The obtained findings let us to assume an involvement of CD+CD+ lymphocytes in pathogenesis of inflammatory alterations at COPD. An increased level of IL-17 was revealed in COPD patients group, versus those smokers, who had no clinical signs of COPD, thus suggesting activation of secretory functions in a subpopulation of CD+CD+ lymphocytes (i.e., Th17).
format	article
author	E. I. Altynbaeva S. N. Teplova S. A. Kochengina
author_facet	E. I. Altynbaeva S. N. Teplova S. A. Kochengina
author_sort	E. I. Altynbaeva
title	QUANTITATIVE EVALUATION OF IMMUNOCYTES AND INTERLEUKIN-17 LEVELS IN SALIVA FROM SMOKERS AT THE EARLY STAGES OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE
title_short	QUANTITATIVE EVALUATION OF IMMUNOCYTES AND INTERLEUKIN-17 LEVELS IN SALIVA FROM SMOKERS AT THE EARLY STAGES OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE
title_full	QUANTITATIVE EVALUATION OF IMMUNOCYTES AND INTERLEUKIN-17 LEVELS IN SALIVA FROM SMOKERS AT THE EARLY STAGES OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE
title_fullStr	QUANTITATIVE EVALUATION OF IMMUNOCYTES AND INTERLEUKIN-17 LEVELS IN SALIVA FROM SMOKERS AT THE EARLY STAGES OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE
title_full_unstemmed	QUANTITATIVE EVALUATION OF IMMUNOCYTES AND INTERLEUKIN-17 LEVELS IN SALIVA FROM SMOKERS AT THE EARLY STAGES OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE
title_sort	quantitative evaluation of immunocytes and interleukin-17 levels in saliva from smokers at the early stages of chronic obstructive pulmonary disease
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/9a460b44d84244b4900d53ecaa1aa2db
work_keys_str_mv	AT eialtynbaeva quantitativeevaluationofimmunocytesandinterleukin17levelsinsalivafromsmokersattheearlystagesofchronicobstructivepulmonarydisease AT snteplova quantitativeevaluationofimmunocytesandinterleukin17levelsinsalivafromsmokersattheearlystagesofchronicobstructivepulmonarydisease AT sakochengina quantitativeevaluationofimmunocytesandinterleukin17levelsinsalivafromsmokersattheearlystagesofchronicobstructivepulmonarydisease
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QUANTITATIVE EVALUATION OF IMMUNOCYTES AND INTERLEUKIN-17 LEVELS IN SALIVA FROM SMOKERS AT THE EARLY STAGES OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE

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