Lipoparticles for Synergistic Chemo-Photodynamic Therapy to Ovarian Carcinoma Cells: In vitro and in vivo Assessments
Sajid Ali,1 Muhammad Umair Amin,1,2 Imran Tariq,1,3 Muhammad Farhan Sohail,4,5 Muhammad Yasir Ali,1,6 Eduard Preis,1 Ghazala Ambreen,1 Shashank Reddy Pinnapireddy,1 Jarmila Jedelská,1 Jens Schäfer,1 Udo Bakowsky1 1Department of Pharmaceutics and Biopharmaceutics, University of Ma...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/9a48d1ef0da24530a73d99c9338f5feb |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:9a48d1ef0da24530a73d99c9338f5feb |
|---|---|
| record_format |
dspace |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
atomic force microscopy facs in vivo in vitro cytotoxicity lipid-polymer hybrid nanoparticles photodynamic therapy plga nanoparticles tem Medicine (General) R5-920 |
| spellingShingle |
atomic force microscopy facs in vivo in vitro cytotoxicity lipid-polymer hybrid nanoparticles photodynamic therapy plga nanoparticles tem Medicine (General) R5-920 Ali S Amin MU Tariq I Sohail MF Ali MY Preis E Ambreen G Pinnapireddy SR Jedelská J Schäfer J Bakowsky U Lipoparticles for Synergistic Chemo-Photodynamic Therapy to Ovarian Carcinoma Cells: In vitro and in vivo Assessments |
| description |
Sajid Ali,1 Muhammad Umair Amin,1,2 Imran Tariq,1,3 Muhammad Farhan Sohail,4,5 Muhammad Yasir Ali,1,6 Eduard Preis,1 Ghazala Ambreen,1 Shashank Reddy Pinnapireddy,1 Jarmila Jedelská,1 Jens Schäfer,1 Udo Bakowsky1 1Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Marburg, Germany; 2Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan; 3Punjab University College of Pharmacy, University of the Punjab, Allama Iqbal Campus, Lahore, Pakistan; 4Riphah Institute of Pharmaceutical Sciences (RIPS), Riphah International University, Lahore, Pakistan; 5Department of Pharmacy, Faculty of Health and Medical Science, University of Copenhagen, Copenhagen, Denmark; 6Faculty of Pharmaceutical Sciences, GC University Faisalabad, Faisalabad, PakistanCorrespondence: Udo BakowskyDepartment of Pharmaceutics and Biopharmaceutics, University of Marburg, Robert Koch Str. 4, Marburg, 35037, GermanyTel + 49 6421 28 25884Fax + 49 6421 28 27016Email ubakowsky@aol.comPurpose: Lipoparticles are the core-shell type lipid-polymer hybrid systems comprising polymeric nanoparticle core enveloped by single or multiple pegylated lipid layers (shell), thereby melding the biomimetic properties of long-circulating vesicles as well as the mechanical advantages of the nanoparticles. The present study was aimed at the development of such an integrated system, combining the photodynamic and chemotherapeutic approaches for the treatment of multidrug-resistant cancers.Methods: For this rationale, two different sized Pirarubicin (THP) loaded poly lactic-co-glycolic acid (PLGA) nanoparticles were prepared by emulsion solvent evaporation technique, whereas liposomes containing Temoporfin (mTHPC) were prepared by lipid film hydration method. Physicochemical and morphological characterizations were done using dynamic light scattering, laser doppler anemometry, atomic force microscopy, and transmission electron microscopy. The quantitative assessment of cell damage was determined using MTT and reactive oxygen species (ROS) assay. The biocompatibility of the nanoformulations was evaluated with serum stability testing, haemocompatibility as well as acute in vivo toxicity using female albino (BALB/c) mice.Results and Conclusion: The mean hydrodynamic diameter of the formulations was found between 108.80 ± 2.10 to 405.70 ± 10.00 nm with the zeta (ζ) potential ranging from − 12.70 ± 1.20 to 5.90 ± 1.10 mV. Based on the physicochemical evaluations, the selected THP nanoparticles were coated with mTHPC liposomes to produce lipid-coated nanoparticles (LCNPs). A significant (p< 0.001) cytotoxicity synergism was evident in LCNPs when irradiated at 652 nm, using an LED device. No incidence of genotoxicity was observed as seen with the comet assay. The LCNPs decreased the generalized in vivo toxicity as compared to the free drugs and was evident from the serum biochemical profile, visceral body index, liver function tests as well as renal function tests. The histopathological examinations of the vital organs revealed no significant evidence of toxicity suggesting the safety and efficacy of our lipid-polymer hybrid system.Keywords: atomic force microscopy, FACS, in vivo, in vitro cytotoxicity, lipid-polymer hybrid nanoparticles, photodynamic therapy, PLGA nanoparticles, TEM |
| format |
article |
| author |
Ali S Amin MU Tariq I Sohail MF Ali MY Preis E Ambreen G Pinnapireddy SR Jedelská J Schäfer J Bakowsky U |
| author_facet |
Ali S Amin MU Tariq I Sohail MF Ali MY Preis E Ambreen G Pinnapireddy SR Jedelská J Schäfer J Bakowsky U |
| author_sort |
Ali S |
| title |
Lipoparticles for Synergistic Chemo-Photodynamic Therapy to Ovarian Carcinoma Cells: In vitro and in vivo Assessments |
| title_short |
Lipoparticles for Synergistic Chemo-Photodynamic Therapy to Ovarian Carcinoma Cells: In vitro and in vivo Assessments |
| title_full |
Lipoparticles for Synergistic Chemo-Photodynamic Therapy to Ovarian Carcinoma Cells: In vitro and in vivo Assessments |
| title_fullStr |
Lipoparticles for Synergistic Chemo-Photodynamic Therapy to Ovarian Carcinoma Cells: In vitro and in vivo Assessments |
| title_full_unstemmed |
Lipoparticles for Synergistic Chemo-Photodynamic Therapy to Ovarian Carcinoma Cells: In vitro and in vivo Assessments |
| title_sort |
lipoparticles for synergistic chemo-photodynamic therapy to ovarian carcinoma cells: in vitro and in vivo assessments |
| publisher |
Dove Medical Press |
| publishDate |
2021 |
| url |
https://doaj.org/article/9a48d1ef0da24530a73d99c9338f5feb |
| work_keys_str_mv |
AT alis lipoparticlesforsynergisticchemophotodynamictherapytoovariancarcinomacellsinvitroandinvivoassessments AT aminmu lipoparticlesforsynergisticchemophotodynamictherapytoovariancarcinomacellsinvitroandinvivoassessments AT tariqi lipoparticlesforsynergisticchemophotodynamictherapytoovariancarcinomacellsinvitroandinvivoassessments AT sohailmf lipoparticlesforsynergisticchemophotodynamictherapytoovariancarcinomacellsinvitroandinvivoassessments AT alimy lipoparticlesforsynergisticchemophotodynamictherapytoovariancarcinomacellsinvitroandinvivoassessments AT preise lipoparticlesforsynergisticchemophotodynamictherapytoovariancarcinomacellsinvitroandinvivoassessments AT ambreeng lipoparticlesforsynergisticchemophotodynamictherapytoovariancarcinomacellsinvitroandinvivoassessments AT pinnapireddysr lipoparticlesforsynergisticchemophotodynamictherapytoovariancarcinomacellsinvitroandinvivoassessments AT jedelskaj lipoparticlesforsynergisticchemophotodynamictherapytoovariancarcinomacellsinvitroandinvivoassessments AT schaferj lipoparticlesforsynergisticchemophotodynamictherapytoovariancarcinomacellsinvitroandinvivoassessments AT bakowskyu lipoparticlesforsynergisticchemophotodynamictherapytoovariancarcinomacellsinvitroandinvivoassessments |
| _version_ |
1718390670270398464 |
| spelling |
oai:doaj.org-article:9a48d1ef0da24530a73d99c9338f5feb2021-12-02T14:39:24ZLipoparticles for Synergistic Chemo-Photodynamic Therapy to Ovarian Carcinoma Cells: In vitro and in vivo Assessments1178-2013https://doaj.org/article/9a48d1ef0da24530a73d99c9338f5feb2021-02-01T00:00:00Zhttps://www.dovepress.com/lipoparticles-for-synergistic-chemo-photodynamic-therapy-to-ovarian-ca-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sajid Ali,1 Muhammad Umair Amin,1,2 Imran Tariq,1,3 Muhammad Farhan Sohail,4,5 Muhammad Yasir Ali,1,6 Eduard Preis,1 Ghazala Ambreen,1 Shashank Reddy Pinnapireddy,1 Jarmila Jedelská,1 Jens Schäfer,1 Udo Bakowsky1 1Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Marburg, Germany; 2Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan; 3Punjab University College of Pharmacy, University of the Punjab, Allama Iqbal Campus, Lahore, Pakistan; 4Riphah Institute of Pharmaceutical Sciences (RIPS), Riphah International University, Lahore, Pakistan; 5Department of Pharmacy, Faculty of Health and Medical Science, University of Copenhagen, Copenhagen, Denmark; 6Faculty of Pharmaceutical Sciences, GC University Faisalabad, Faisalabad, PakistanCorrespondence: Udo BakowskyDepartment of Pharmaceutics and Biopharmaceutics, University of Marburg, Robert Koch Str. 4, Marburg, 35037, GermanyTel + 49 6421 28 25884Fax + 49 6421 28 27016Email ubakowsky@aol.comPurpose: Lipoparticles are the core-shell type lipid-polymer hybrid systems comprising polymeric nanoparticle core enveloped by single or multiple pegylated lipid layers (shell), thereby melding the biomimetic properties of long-circulating vesicles as well as the mechanical advantages of the nanoparticles. The present study was aimed at the development of such an integrated system, combining the photodynamic and chemotherapeutic approaches for the treatment of multidrug-resistant cancers.Methods: For this rationale, two different sized Pirarubicin (THP) loaded poly lactic-co-glycolic acid (PLGA) nanoparticles were prepared by emulsion solvent evaporation technique, whereas liposomes containing Temoporfin (mTHPC) were prepared by lipid film hydration method. Physicochemical and morphological characterizations were done using dynamic light scattering, laser doppler anemometry, atomic force microscopy, and transmission electron microscopy. The quantitative assessment of cell damage was determined using MTT and reactive oxygen species (ROS) assay. The biocompatibility of the nanoformulations was evaluated with serum stability testing, haemocompatibility as well as acute in vivo toxicity using female albino (BALB/c) mice.Results and Conclusion: The mean hydrodynamic diameter of the formulations was found between 108.80 ± 2.10 to 405.70 ± 10.00 nm with the zeta (ζ) potential ranging from − 12.70 ± 1.20 to 5.90 ± 1.10 mV. Based on the physicochemical evaluations, the selected THP nanoparticles were coated with mTHPC liposomes to produce lipid-coated nanoparticles (LCNPs). A significant (p< 0.001) cytotoxicity synergism was evident in LCNPs when irradiated at 652 nm, using an LED device. No incidence of genotoxicity was observed as seen with the comet assay. The LCNPs decreased the generalized in vivo toxicity as compared to the free drugs and was evident from the serum biochemical profile, visceral body index, liver function tests as well as renal function tests. The histopathological examinations of the vital organs revealed no significant evidence of toxicity suggesting the safety and efficacy of our lipid-polymer hybrid system.Keywords: atomic force microscopy, FACS, in vivo, in vitro cytotoxicity, lipid-polymer hybrid nanoparticles, photodynamic therapy, PLGA nanoparticles, TEMAli SAmin MUTariq ISohail MFAli MYPreis EAmbreen GPinnapireddy SRJedelská JSchäfer JBakowsky UDove Medical Pressarticleatomic force microscopyfacsin vivoin vitro cytotoxicitylipid-polymer hybrid nanoparticlesphotodynamic therapyplga nanoparticlestemMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 951-976 (2021) |