NanoUPLC-QTOF-MS/MS Determination of Major Rosuvastatin Degradation Products Generated by Gamma Radiation in Aqueous Solution

Rosuvastatin, a member of the statin family of drugs, is used to regulate high cholesterol levels in the human body. Moreover, rosuvastatin and other statins demonstrate a protective role against free radical-induced oxidative stress. Our research aimed to investigate the end-products of free radica...

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Autores principales:	Lucija Dončević, Ema Svetličić, Amela Hozić, Branka Mihaljević, Dorota Jarmużek, Ivana Tartaro Bujak, Donata Pluskota-Karwatka, Luka Ozdanovac, Iva Džeba, Mario Cindrić
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	rosuvastatin nanoLC-MS gamma radiation degradation products identification OH radical mechanisms Medicine R Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/9a49ffc6112f47cba51d155282fd216d
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spelling	oai:doaj.org-article:9a49ffc6112f47cba51d155282fd216d2021-11-25T18:39:49ZNanoUPLC-QTOF-MS/MS Determination of Major Rosuvastatin Degradation Products Generated by Gamma Radiation in Aqueous Solution10.3390/ph141111601424-8247https://doaj.org/article/9a49ffc6112f47cba51d155282fd216d2021-11-01T00:00:00Zhttps://www.mdpi.com/1424-8247/14/11/1160https://doaj.org/toc/1424-8247Rosuvastatin, a member of the statin family of drugs, is used to regulate high cholesterol levels in the human body. Moreover, rosuvastatin and other statins demonstrate a protective role against free radical-induced oxidative stress. Our research aimed to investigate the end-products of free radical-induced degradation of rosuvastatin. To induce the radical degradation, an aqueous solution of rosuvastatin was irradiated using different doses of gamma radiation (50–1000 Gy) under oxidative conditions. Rosuvastatin and related degradation products were separated on nanoC18 column under gradient elution, and identification was carried out on hyphenated nanoUPLC and nanoESI-QTOF mass spectrometer system. Elemental composition analysis using highly accurate mass measurements together with isotope fitting algorithm identified nine major degradation products. This is the first study of gamma radiation-induced degradation of rosuvastatin, where chemical structures, MS/MS fragmentation pathways and formation mechanisms of the resulting degradation products are detailly described. The presented results contribute to the understanding of the degradation pathway of rosuvastatin and possibly other statins under gamma radiation conditions.Lucija DončevićEma SvetličićAmela HozićBranka MihaljevićDorota JarmużekIvana Tartaro BujakDonata Pluskota-KarwatkaLuka OzdanovacIva DžebaMario CindrićMDPI AGarticlerosuvastatinnanoLC-MSgamma radiationdegradation products identificationOH radical mechanismsMedicineRPharmacy and materia medicaRS1-441ENPharmaceuticals, Vol 14, Iss 1160, p 1160 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rosuvastatin nanoLC-MS gamma radiation degradation products identification OH radical mechanisms Medicine R Pharmacy and materia medica RS1-441
spellingShingle	rosuvastatin nanoLC-MS gamma radiation degradation products identification OH radical mechanisms Medicine R Pharmacy and materia medica RS1-441 Lucija Dončević Ema Svetličić Amela Hozić Branka Mihaljević Dorota Jarmużek Ivana Tartaro Bujak Donata Pluskota-Karwatka Luka Ozdanovac Iva Džeba Mario Cindrić NanoUPLC-QTOF-MS/MS Determination of Major Rosuvastatin Degradation Products Generated by Gamma Radiation in Aqueous Solution
description	Rosuvastatin, a member of the statin family of drugs, is used to regulate high cholesterol levels in the human body. Moreover, rosuvastatin and other statins demonstrate a protective role against free radical-induced oxidative stress. Our research aimed to investigate the end-products of free radical-induced degradation of rosuvastatin. To induce the radical degradation, an aqueous solution of rosuvastatin was irradiated using different doses of gamma radiation (50–1000 Gy) under oxidative conditions. Rosuvastatin and related degradation products were separated on nanoC18 column under gradient elution, and identification was carried out on hyphenated nanoUPLC and nanoESI-QTOF mass spectrometer system. Elemental composition analysis using highly accurate mass measurements together with isotope fitting algorithm identified nine major degradation products. This is the first study of gamma radiation-induced degradation of rosuvastatin, where chemical structures, MS/MS fragmentation pathways and formation mechanisms of the resulting degradation products are detailly described. The presented results contribute to the understanding of the degradation pathway of rosuvastatin and possibly other statins under gamma radiation conditions.
format	article
author	Lucija Dončević Ema Svetličić Amela Hozić Branka Mihaljević Dorota Jarmużek Ivana Tartaro Bujak Donata Pluskota-Karwatka Luka Ozdanovac Iva Džeba Mario Cindrić
author_facet	Lucija Dončević Ema Svetličić Amela Hozić Branka Mihaljević Dorota Jarmużek Ivana Tartaro Bujak Donata Pluskota-Karwatka Luka Ozdanovac Iva Džeba Mario Cindrić
author_sort	Lucija Dončević
title	NanoUPLC-QTOF-MS/MS Determination of Major Rosuvastatin Degradation Products Generated by Gamma Radiation in Aqueous Solution
title_short	NanoUPLC-QTOF-MS/MS Determination of Major Rosuvastatin Degradation Products Generated by Gamma Radiation in Aqueous Solution
title_full	NanoUPLC-QTOF-MS/MS Determination of Major Rosuvastatin Degradation Products Generated by Gamma Radiation in Aqueous Solution
title_fullStr	NanoUPLC-QTOF-MS/MS Determination of Major Rosuvastatin Degradation Products Generated by Gamma Radiation in Aqueous Solution
title_full_unstemmed	NanoUPLC-QTOF-MS/MS Determination of Major Rosuvastatin Degradation Products Generated by Gamma Radiation in Aqueous Solution
title_sort	nanouplc-qtof-ms/ms determination of major rosuvastatin degradation products generated by gamma radiation in aqueous solution
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9a49ffc6112f47cba51d155282fd216d
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NanoUPLC-QTOF-MS/MS Determination of Major Rosuvastatin Degradation Products Generated by Gamma Radiation in Aqueous Solution

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