Assessment of the Anti-apoptotic Effects of Peganum harmala Leaf Extract on Type 2 Diabetes in the Kidney of Male Wistar Rats

Background: Growing evidence has shown that the apoptosis of cells plays an important role in the advancement of the Diabetic nephropathy (DN). Objectives: This study attempted to discover the therapeutic potential of Peganum harmala leaf extract in the apoptosis of diabetic kidney disease. Me...

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Autores principales:	Forough Kajbaf, Shahrbanoo Oryan, Ramesh Ahmadi, Akram Eidi
Formato:	article
Lenguaje:	EN
Publicado:	Hamadan University of Medical Sciences 2020
Materias:	apoptosis peganum harmala diabetes kidney caspase-3 Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/9a55d30cb8e2473ba312a3ee22135a90
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spelling	oai:doaj.org-article:9a55d30cb8e2473ba312a3ee22135a902021-11-23T07:31:00ZAssessment of the Anti-apoptotic Effects of Peganum harmala Leaf Extract on Type 2 Diabetes in the Kidney of Male Wistar Rats10.34172/ajmb.2020.112345-4113https://doaj.org/article/9a55d30cb8e2473ba312a3ee22135a902020-12-01T00:00:00Zhttp://ajmb.umsha.ac.ir/PDF/ajmb-8-74.pdfhttps://doaj.org/toc/2345-4113Background: Growing evidence has shown that the apoptosis of cells plays an important role in the advancement of the Diabetic nephropathy (DN). Objectives: This study attempted to discover the therapeutic potential of Peganum harmala leaf extract in the apoptosis of diabetic kidney disease. Methods: In the present experimental research, 32 male Wistar rats were studied, and diabetes was induced by streptozotocin (STZ) (65 mg/kg). The animals were randomly divided into four groups (n=8, in each group) as follows: control, diabetic, control+leaf extract, diabetic+leaf extract. For our purposes, the methanolic extract of P. harmala leaves (150 mg/kg) was given by gavage for 28 days. Flow cytometry and real-time polymerase chain reaction (PCR) analyses were utilized to determine the percentages of apoptotic cells. Also, histological alterations and blood biochemical parameters were evaluated. Results: The P. harmala leaf extract has a high amount of flavonoids (25.84%), a lower percentage of alkaloids (0.14%), and some antioxidant properties. Serum urea (P<0.001) and apoptosis (P<0.05) significantly elevated in diabetic rats relative to the control ones. The mean of fasting blood creatinine, urea, and albumin level was not significantly changed in diabetic+leaf extract rats as compared to the diabetic ones. Histopathological results also displayed that diabetic complications in the kidney could not be improved following treatment by the leaf extract of P. harmala. In addition, the leaf extract could not significantly reduce the apoptosis and caspase-3 expression compared to diabetics in renal cells. Conclusion: Based on our findings, the leaf extract of P. harmala is unable to inhibit apoptosis in the diabetic kidney model.Forough KajbafShahrbanoo OryanRamesh AhmadiAkram EidiHamadan University of Medical Sciencesarticleapoptosispeganum harmaladiabeteskidneycaspase-3Medical technologyR855-855.5ENAvicenna Journal of Medical Biochemistry, Vol 8, Iss 2, Pp 74-82 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	apoptosis peganum harmala diabetes kidney caspase-3 Medical technology R855-855.5
spellingShingle	apoptosis peganum harmala diabetes kidney caspase-3 Medical technology R855-855.5 Forough Kajbaf Shahrbanoo Oryan Ramesh Ahmadi Akram Eidi Assessment of the Anti-apoptotic Effects of Peganum harmala Leaf Extract on Type 2 Diabetes in the Kidney of Male Wistar Rats
description	Background: Growing evidence has shown that the apoptosis of cells plays an important role in the advancement of the Diabetic nephropathy (DN). Objectives: This study attempted to discover the therapeutic potential of Peganum harmala leaf extract in the apoptosis of diabetic kidney disease. Methods: In the present experimental research, 32 male Wistar rats were studied, and diabetes was induced by streptozotocin (STZ) (65 mg/kg). The animals were randomly divided into four groups (n=8, in each group) as follows: control, diabetic, control+leaf extract, diabetic+leaf extract. For our purposes, the methanolic extract of P. harmala leaves (150 mg/kg) was given by gavage for 28 days. Flow cytometry and real-time polymerase chain reaction (PCR) analyses were utilized to determine the percentages of apoptotic cells. Also, histological alterations and blood biochemical parameters were evaluated. Results: The P. harmala leaf extract has a high amount of flavonoids (25.84%), a lower percentage of alkaloids (0.14%), and some antioxidant properties. Serum urea (P<0.001) and apoptosis (P<0.05) significantly elevated in diabetic rats relative to the control ones. The mean of fasting blood creatinine, urea, and albumin level was not significantly changed in diabetic+leaf extract rats as compared to the diabetic ones. Histopathological results also displayed that diabetic complications in the kidney could not be improved following treatment by the leaf extract of P. harmala. In addition, the leaf extract could not significantly reduce the apoptosis and caspase-3 expression compared to diabetics in renal cells. Conclusion: Based on our findings, the leaf extract of P. harmala is unable to inhibit apoptosis in the diabetic kidney model.
format	article
author	Forough Kajbaf Shahrbanoo Oryan Ramesh Ahmadi Akram Eidi
author_facet	Forough Kajbaf Shahrbanoo Oryan Ramesh Ahmadi Akram Eidi
author_sort	Forough Kajbaf
title	Assessment of the Anti-apoptotic Effects of Peganum harmala Leaf Extract on Type 2 Diabetes in the Kidney of Male Wistar Rats
title_short	Assessment of the Anti-apoptotic Effects of Peganum harmala Leaf Extract on Type 2 Diabetes in the Kidney of Male Wistar Rats
title_full	Assessment of the Anti-apoptotic Effects of Peganum harmala Leaf Extract on Type 2 Diabetes in the Kidney of Male Wistar Rats
title_fullStr	Assessment of the Anti-apoptotic Effects of Peganum harmala Leaf Extract on Type 2 Diabetes in the Kidney of Male Wistar Rats
title_full_unstemmed	Assessment of the Anti-apoptotic Effects of Peganum harmala Leaf Extract on Type 2 Diabetes in the Kidney of Male Wistar Rats
title_sort	assessment of the anti-apoptotic effects of peganum harmala leaf extract on type 2 diabetes in the kidney of male wistar rats
publisher	Hamadan University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/9a55d30cb8e2473ba312a3ee22135a90
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Assessment of the Anti-apoptotic Effects of Peganum harmala Leaf Extract on Type 2 Diabetes in the Kidney of Male Wistar Rats

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