Analysis of the frequency of single nucleotide polymorphisms in cytokine genes in patients with New Onset Diabetes After Transplant

Abstract New Onset Diabetes After Transplantation (NODAT) is a serious metabolic complication. While β-cell dysfunction is considered the main contributing factor in the development of NODAT, the precise pathogenesis is not well understood. Cytokines are thought to be involved in the inflammation of...

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Autores principales:	Mohamed Jahromi, Torki Al-Otaibi, Osama Ashry Gheith, Nashwa Farouk Othman, Tarek Mahmoud, Parasad Nair, Medhat A-Halim, Parul Aggarwal, Grace Messenger, Philip Chu, Sacha A. De Serres, Jamil R. Azzi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9a574fadf06144d58b53f029e56d34f5
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spelling	oai:doaj.org-article:9a574fadf06144d58b53f029e56d34f52021-12-02T16:30:47ZAnalysis of the frequency of single nucleotide polymorphisms in cytokine genes in patients with New Onset Diabetes After Transplant10.1038/s41598-021-84400-92045-2322https://doaj.org/article/9a574fadf06144d58b53f029e56d34f52021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84400-9https://doaj.org/toc/2045-2322Abstract New Onset Diabetes After Transplantation (NODAT) is a serious metabolic complication. While β-cell dysfunction is considered the main contributing factor in the development of NODAT, the precise pathogenesis is not well understood. Cytokines are thought to be involved in the inflammation of islet β-cells in diabetes; however, few studies have investigated this hypothesis in NODAT. A total of 309 kidney transplant recipients (KTRs) were included in this study. An association between kidney transplants, and the development of diabetes after transplant (NODAT) was investigated. Comparison was made between KTRs who develop diabetes (NODAT cases) or did not develop diabetes (control), using key cytokines, IL-6 G (− 174)C, macrophage mediator; IL-4 C (− 490)T, T helper (Th)-2 cytokine profile initiator; Th-1 cytokine profile initiator interferon-γ T (+ 874) A gene and TGF β1 C (+ 869) T gene polymorphisms were investigated. The genes were amplified using well-established polymerase chain reaction (PCR) techniques in our laboratory. Compared to the AA and AT genotypes of interferon gamma (IFNG), there was a strong association between the TT genotype of IFNG and NODAT kidney transplant recipients (KTRs) versus non-NODAT KTRs (p = 0.005). The AA genotype of IFNG was found to be predominant in the control group (p = 0.004). Also, significant variations of IL6 G (− 174) C, IL-4 C (− 590) T, interferon-γ T (+ 874) A gene and transforming growth factor β1 C (+ 869) T may contribute to NODAT. Our data is consistent with theTh-1/T-reg pathway of immunity. Further larger pan Arab studies are required to confirm our findings.Mohamed JahromiTorki Al-OtaibiOsama Ashry GheithNashwa Farouk OthmanTarek MahmoudParasad NairMedhat A-HalimParul AggarwalGrace MessengerPhilip ChuSacha A. De SerresJamil R. AzziNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mohamed Jahromi Torki Al-Otaibi Osama Ashry Gheith Nashwa Farouk Othman Tarek Mahmoud Parasad Nair Medhat A-Halim Parul Aggarwal Grace Messenger Philip Chu Sacha A. De Serres Jamil R. Azzi Analysis of the frequency of single nucleotide polymorphisms in cytokine genes in patients with New Onset Diabetes After Transplant
description	Abstract New Onset Diabetes After Transplantation (NODAT) is a serious metabolic complication. While β-cell dysfunction is considered the main contributing factor in the development of NODAT, the precise pathogenesis is not well understood. Cytokines are thought to be involved in the inflammation of islet β-cells in diabetes; however, few studies have investigated this hypothesis in NODAT. A total of 309 kidney transplant recipients (KTRs) were included in this study. An association between kidney transplants, and the development of diabetes after transplant (NODAT) was investigated. Comparison was made between KTRs who develop diabetes (NODAT cases) or did not develop diabetes (control), using key cytokines, IL-6 G (− 174)C, macrophage mediator; IL-4 C (− 490)T, T helper (Th)-2 cytokine profile initiator; Th-1 cytokine profile initiator interferon-γ T (+ 874) A gene and TGF β1 C (+ 869) T gene polymorphisms were investigated. The genes were amplified using well-established polymerase chain reaction (PCR) techniques in our laboratory. Compared to the AA and AT genotypes of interferon gamma (IFNG), there was a strong association between the TT genotype of IFNG and NODAT kidney transplant recipients (KTRs) versus non-NODAT KTRs (p = 0.005). The AA genotype of IFNG was found to be predominant in the control group (p = 0.004). Also, significant variations of IL6 G (− 174) C, IL-4 C (− 590) T, interferon-γ T (+ 874) A gene and transforming growth factor β1 C (+ 869) T may contribute to NODAT. Our data is consistent with theTh-1/T-reg pathway of immunity. Further larger pan Arab studies are required to confirm our findings.
format	article
author	Mohamed Jahromi Torki Al-Otaibi Osama Ashry Gheith Nashwa Farouk Othman Tarek Mahmoud Parasad Nair Medhat A-Halim Parul Aggarwal Grace Messenger Philip Chu Sacha A. De Serres Jamil R. Azzi
author_facet	Mohamed Jahromi Torki Al-Otaibi Osama Ashry Gheith Nashwa Farouk Othman Tarek Mahmoud Parasad Nair Medhat A-Halim Parul Aggarwal Grace Messenger Philip Chu Sacha A. De Serres Jamil R. Azzi
author_sort	Mohamed Jahromi
title	Analysis of the frequency of single nucleotide polymorphisms in cytokine genes in patients with New Onset Diabetes After Transplant
title_short	Analysis of the frequency of single nucleotide polymorphisms in cytokine genes in patients with New Onset Diabetes After Transplant
title_full	Analysis of the frequency of single nucleotide polymorphisms in cytokine genes in patients with New Onset Diabetes After Transplant
title_fullStr	Analysis of the frequency of single nucleotide polymorphisms in cytokine genes in patients with New Onset Diabetes After Transplant
title_full_unstemmed	Analysis of the frequency of single nucleotide polymorphisms in cytokine genes in patients with New Onset Diabetes After Transplant
title_sort	analysis of the frequency of single nucleotide polymorphisms in cytokine genes in patients with new onset diabetes after transplant
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9a574fadf06144d58b53f029e56d34f5
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Analysis of the frequency of single nucleotide polymorphisms in cytokine genes in patients with New Onset Diabetes After Transplant

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