The channel-activating protease CAP1/Prss8 is required for placental labyrinth maturation.

The serine protease CAP1/Prss8 is crucial for skin barrier function, lung alveolar fluid clearance and has been unveiled as diagnostic marker for specific cancer types. Here, we show that a constitutive knockout of CAP1/Prss8 leads to embryonic lethality. These embryos presented no specific defects,...

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Autores principales:	Edith Hummler, Aline Dousse, Audrey Rieder, Jean-Christophe Stehle, Isabelle Rubera, Maria-Chiara Osterheld, Friedrich Beermann, Simona Frateschi, Roch-Philippe Charles
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9a580cb873b145eba6aa52e64ed5ac1b
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spelling	oai:doaj.org-article:9a580cb873b145eba6aa52e64ed5ac1b2021-11-18T07:58:31ZThe channel-activating protease CAP1/Prss8 is required for placental labyrinth maturation.1932-620310.1371/journal.pone.0055796https://doaj.org/article/9a580cb873b145eba6aa52e64ed5ac1b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23405214/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The serine protease CAP1/Prss8 is crucial for skin barrier function, lung alveolar fluid clearance and has been unveiled as diagnostic marker for specific cancer types. Here, we show that a constitutive knockout of CAP1/Prss8 leads to embryonic lethality. These embryos presented no specific defects, but it is during this period, and in particular at E13.5, that wildtype placentas show an increased expression of CAP1/Prss8, thus suggesting a placental defect in the knockout situation. The placentas of knockout embryos exhibited significantly reduced vascular development and incomplete cellular maturation. In contrary, epiblast-specific deletion of CAP1/Prss8 allowed development until birth. These CAP1/Prss8-deficient newborns presented abnormal epidermis, and died soon after birth due to impaired skin function. We thus conclude that a late placental insufficiency might be the primary cause of embryonic lethality in CAP1/Prss8 knockouts. This study highlights a novel and crucial role for CAP1/Prss8 in placental development and function.Edith HummlerAline DousseAudrey RiederJean-Christophe StehleIsabelle RuberaMaria-Chiara OsterheldFriedrich BeermannSimona FrateschiRoch-Philippe CharlesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e55796 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Edith Hummler Aline Dousse Audrey Rieder Jean-Christophe Stehle Isabelle Rubera Maria-Chiara Osterheld Friedrich Beermann Simona Frateschi Roch-Philippe Charles The channel-activating protease CAP1/Prss8 is required for placental labyrinth maturation.
description	The serine protease CAP1/Prss8 is crucial for skin barrier function, lung alveolar fluid clearance and has been unveiled as diagnostic marker for specific cancer types. Here, we show that a constitutive knockout of CAP1/Prss8 leads to embryonic lethality. These embryos presented no specific defects, but it is during this period, and in particular at E13.5, that wildtype placentas show an increased expression of CAP1/Prss8, thus suggesting a placental defect in the knockout situation. The placentas of knockout embryos exhibited significantly reduced vascular development and incomplete cellular maturation. In contrary, epiblast-specific deletion of CAP1/Prss8 allowed development until birth. These CAP1/Prss8-deficient newborns presented abnormal epidermis, and died soon after birth due to impaired skin function. We thus conclude that a late placental insufficiency might be the primary cause of embryonic lethality in CAP1/Prss8 knockouts. This study highlights a novel and crucial role for CAP1/Prss8 in placental development and function.
format	article
author	Edith Hummler Aline Dousse Audrey Rieder Jean-Christophe Stehle Isabelle Rubera Maria-Chiara Osterheld Friedrich Beermann Simona Frateschi Roch-Philippe Charles
author_facet	Edith Hummler Aline Dousse Audrey Rieder Jean-Christophe Stehle Isabelle Rubera Maria-Chiara Osterheld Friedrich Beermann Simona Frateschi Roch-Philippe Charles
author_sort	Edith Hummler
title	The channel-activating protease CAP1/Prss8 is required for placental labyrinth maturation.
title_short	The channel-activating protease CAP1/Prss8 is required for placental labyrinth maturation.
title_full	The channel-activating protease CAP1/Prss8 is required for placental labyrinth maturation.
title_fullStr	The channel-activating protease CAP1/Prss8 is required for placental labyrinth maturation.
title_full_unstemmed	The channel-activating protease CAP1/Prss8 is required for placental labyrinth maturation.
title_sort	channel-activating protease cap1/prss8 is required for placental labyrinth maturation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/9a580cb873b145eba6aa52e64ed5ac1b
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The channel-activating protease CAP1/Prss8 is required for placental labyrinth maturation.

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