Novel H2S donor proglumide-ADT-OH protects HUVECs from ox-LDL-induced injury through NF-κB and JAK/SATA pathway

As a gaseous mediator, hydrogen sulfide (H2S) has many physiological effects and pathological effects in atherosclerosis. In recent years, many exogenous H2S donors have been synthesized to study atherosclerosis diseases. In this study, proglumide-(5-(4-hydroxyphenyl)-3H-1,2-dithiole-3-thione) (P-A)...

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Autores principales:	Ou Xuelan, Xia Tianqin, Yang Chunyan, Yu Chunlei, Zhang Shipeng, Huang Rong, Chen Chuan, Zhou Chunyang
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	hydrogen sulfide donor atherosclerosis nf-кb jak/stat proglumide-(5-(4-hydroxyphenyl)-3h-1,2-dithiole-3-thione) Medicine R
Acceso en línea:	https://doaj.org/article/9a5ab978e3f24fe68f06f77a78188695
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spelling	oai:doaj.org-article:9a5ab978e3f24fe68f06f77a781886952021-12-05T14:10:54ZNovel H2S donor proglumide-ADT-OH protects HUVECs from ox-LDL-induced injury through NF-κB and JAK/SATA pathway2391-546310.1515/med-2021-0287https://doaj.org/article/9a5ab978e3f24fe68f06f77a781886952021-09-01T00:00:00Zhttps://doi.org/10.1515/med-2021-0287https://doaj.org/toc/2391-5463As a gaseous mediator, hydrogen sulfide (H2S) has many physiological effects and pathological effects in atherosclerosis. In recent years, many exogenous H2S donors have been synthesized to study atherosclerosis diseases. In this study, proglumide-(5-(4-hydroxyphenyl)-3H-1,2-dithiole-3-thione) (P-A) was synthesized as a H2S donor. The protective effect and mechanism of P-A on HUVEC that was injured by ox-LDL were detected. The HUEVCs were affected by 100 μmol/L P-A for 24 h; the release of H2S was the largest. After 100 μmol/L P-A acted on HUVEC damage model for 12 h, the cell proliferation activity was the best. The results showed that P-A can downregulate the expression of p-NF-кBp65 protein and reduce the amount of TNF-α and IL-6 and promote the formation of IL-10 by inhibiting the NF-кB pathway, and also induce the expression of superoxide dismutase (SOD) to protect HUVEC from ox-LDL injury. P-A can also regulate JAK/STAT pathway to reduce the expression of p-JAK2 protein and reduce the production of IL-6 and TNF-α. P-A has protective effect on HUVEC injured by ox-LDL, and the protective mechanism is related to the regulation of JAK/STAT pathway and NF-кB pathway.Ou XuelanXia TianqinYang ChunyanYu ChunleiZhang ShipengHuang RongChen ChuanZhou ChunyangDe Gruyterarticlehydrogen sulfide donoratherosclerosisnf-кbjak/statproglumide-(5-(4-hydroxyphenyl)-3h-1,2-dithiole-3-thione)MedicineRENOpen Medicine, Vol 16, Iss 1, Pp 1318-1327 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hydrogen sulfide donor atherosclerosis nf-кb jak/stat proglumide-(5-(4-hydroxyphenyl)-3h-1,2-dithiole-3-thione) Medicine R
spellingShingle	hydrogen sulfide donor atherosclerosis nf-кb jak/stat proglumide-(5-(4-hydroxyphenyl)-3h-1,2-dithiole-3-thione) Medicine R Ou Xuelan Xia Tianqin Yang Chunyan Yu Chunlei Zhang Shipeng Huang Rong Chen Chuan Zhou Chunyang Novel H2S donor proglumide-ADT-OH protects HUVECs from ox-LDL-induced injury through NF-κB and JAK/SATA pathway
description	As a gaseous mediator, hydrogen sulfide (H2S) has many physiological effects and pathological effects in atherosclerosis. In recent years, many exogenous H2S donors have been synthesized to study atherosclerosis diseases. In this study, proglumide-(5-(4-hydroxyphenyl)-3H-1,2-dithiole-3-thione) (P-A) was synthesized as a H2S donor. The protective effect and mechanism of P-A on HUVEC that was injured by ox-LDL were detected. The HUEVCs were affected by 100 μmol/L P-A for 24 h; the release of H2S was the largest. After 100 μmol/L P-A acted on HUVEC damage model for 12 h, the cell proliferation activity was the best. The results showed that P-A can downregulate the expression of p-NF-кBp65 protein and reduce the amount of TNF-α and IL-6 and promote the formation of IL-10 by inhibiting the NF-кB pathway, and also induce the expression of superoxide dismutase (SOD) to protect HUVEC from ox-LDL injury. P-A can also regulate JAK/STAT pathway to reduce the expression of p-JAK2 protein and reduce the production of IL-6 and TNF-α. P-A has protective effect on HUVEC injured by ox-LDL, and the protective mechanism is related to the regulation of JAK/STAT pathway and NF-кB pathway.
format	article
author	Ou Xuelan Xia Tianqin Yang Chunyan Yu Chunlei Zhang Shipeng Huang Rong Chen Chuan Zhou Chunyang
author_facet	Ou Xuelan Xia Tianqin Yang Chunyan Yu Chunlei Zhang Shipeng Huang Rong Chen Chuan Zhou Chunyang
author_sort	Ou Xuelan
title	Novel H2S donor proglumide-ADT-OH protects HUVECs from ox-LDL-induced injury through NF-κB and JAK/SATA pathway
title_short	Novel H2S donor proglumide-ADT-OH protects HUVECs from ox-LDL-induced injury through NF-κB and JAK/SATA pathway
title_full	Novel H2S donor proglumide-ADT-OH protects HUVECs from ox-LDL-induced injury through NF-κB and JAK/SATA pathway
title_fullStr	Novel H2S donor proglumide-ADT-OH protects HUVECs from ox-LDL-induced injury through NF-κB and JAK/SATA pathway
title_full_unstemmed	Novel H2S donor proglumide-ADT-OH protects HUVECs from ox-LDL-induced injury through NF-κB and JAK/SATA pathway
title_sort	novel h2s donor proglumide-adt-oh protects huvecs from ox-ldl-induced injury through nf-κb and jak/sata pathway
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/9a5ab978e3f24fe68f06f77a78188695
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Novel H2S donor proglumide-ADT-OH protects HUVECs from ox-LDL-induced injury through NF-κB and JAK/SATA pathway

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