Transcriptome and metabolite analysis identifies nitrogen utilization genes in tea plant (Camellia sinensis)

Abstract Applied nitrogen (N) fertilizer significantly increases the leaf yield. However, most N is not utilized by the plant, negatively impacting the environment. To date, little is known regarding N utilization genes and mechanisms in the leaf production. To understand this, we investigated trans...

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Autores principales:	Wei Li, Fen Xiang, Micai Zhong, Lingyun Zhou, Hongyan Liu, Saijun Li, Xuewen Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9a5e490b4eb242789860b9f436184505
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spelling	oai:doaj.org-article:9a5e490b4eb242789860b9f4361845052021-12-02T11:40:51ZTranscriptome and metabolite analysis identifies nitrogen utilization genes in tea plant (Camellia sinensis)10.1038/s41598-017-01949-02045-2322https://doaj.org/article/9a5e490b4eb242789860b9f4361845052017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01949-0https://doaj.org/toc/2045-2322Abstract Applied nitrogen (N) fertilizer significantly increases the leaf yield. However, most N is not utilized by the plant, negatively impacting the environment. To date, little is known regarding N utilization genes and mechanisms in the leaf production. To understand this, we investigated transcriptomes using RNA-seq and amino acid levels with N treatment in tea (Camellia sinensis), the most popular beverage crop. We identified 196 and 29 common differentially expressed genes in roots and leaves, respectively, in response to ammonium in two tea varieties. Among those genes, AMT, NRT and AQP for N uptake and GOGAT and GS for N assimilation were the key genes, validated by RT-qPCR, which expressed in a network manner with tissue specificity. Importantly, only AQP and three novel DEGs associated with stress, manganese binding, and gibberellin-regulated transcription factor were common in N responses across all tissues and varieties. A hypothesized gene regulatory network for N was proposed. A strong statistical correlation between key genes’ expression and amino acid content was revealed. The key genes and regulatory network improve our understanding of the molecular mechanism of N usage and offer gene targets for plant improvement.Wei LiFen XiangMicai ZhongLingyun ZhouHongyan LiuSaijun LiXuewen WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wei Li Fen Xiang Micai Zhong Lingyun Zhou Hongyan Liu Saijun Li Xuewen Wang Transcriptome and metabolite analysis identifies nitrogen utilization genes in tea plant (Camellia sinensis)
description	Abstract Applied nitrogen (N) fertilizer significantly increases the leaf yield. However, most N is not utilized by the plant, negatively impacting the environment. To date, little is known regarding N utilization genes and mechanisms in the leaf production. To understand this, we investigated transcriptomes using RNA-seq and amino acid levels with N treatment in tea (Camellia sinensis), the most popular beverage crop. We identified 196 and 29 common differentially expressed genes in roots and leaves, respectively, in response to ammonium in two tea varieties. Among those genes, AMT, NRT and AQP for N uptake and GOGAT and GS for N assimilation were the key genes, validated by RT-qPCR, which expressed in a network manner with tissue specificity. Importantly, only AQP and three novel DEGs associated with stress, manganese binding, and gibberellin-regulated transcription factor were common in N responses across all tissues and varieties. A hypothesized gene regulatory network for N was proposed. A strong statistical correlation between key genes’ expression and amino acid content was revealed. The key genes and regulatory network improve our understanding of the molecular mechanism of N usage and offer gene targets for plant improvement.
format	article
author	Wei Li Fen Xiang Micai Zhong Lingyun Zhou Hongyan Liu Saijun Li Xuewen Wang
author_facet	Wei Li Fen Xiang Micai Zhong Lingyun Zhou Hongyan Liu Saijun Li Xuewen Wang
author_sort	Wei Li
title	Transcriptome and metabolite analysis identifies nitrogen utilization genes in tea plant (Camellia sinensis)
title_short	Transcriptome and metabolite analysis identifies nitrogen utilization genes in tea plant (Camellia sinensis)
title_full	Transcriptome and metabolite analysis identifies nitrogen utilization genes in tea plant (Camellia sinensis)
title_fullStr	Transcriptome and metabolite analysis identifies nitrogen utilization genes in tea plant (Camellia sinensis)
title_full_unstemmed	Transcriptome and metabolite analysis identifies nitrogen utilization genes in tea plant (Camellia sinensis)
title_sort	transcriptome and metabolite analysis identifies nitrogen utilization genes in tea plant (camellia sinensis)
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9a5e490b4eb242789860b9f436184505
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Transcriptome and metabolite analysis identifies nitrogen utilization genes in tea plant (Camellia sinensis)

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