High-resolution ultramicroscopy of the developing and adult nervous system in optically cleared Drosophila melanogaster
Optical aberrations due to pigments in the eye and cuticle have undermined the ability for high-resolution imaging of the intact Drosophila. Here, the authors report an improved tissue-clearing agent, light-sheet optics and a multi-view combining algorithm to overcome these limitations.
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| Autores principales: | , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2018
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/9a6600624b624c8a9084b66847032960 |
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| Sumario: | Optical aberrations due to pigments in the eye and cuticle have undermined the ability for high-resolution imaging of the intact Drosophila. Here, the authors report an improved tissue-clearing agent, light-sheet optics and a multi-view combining algorithm to overcome these limitations. |
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