High-resolution ultramicroscopy of the developing and adult nervous system in optically cleared Drosophila melanogaster

Optical aberrations due to pigments in the eye and cuticle have undermined the ability for high-resolution imaging of the intact Drosophila. Here, the authors report an improved tissue-clearing agent, light-sheet optics and a multi-view combining algorithm to overcome these limitations.

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Autores principales:	Marko Pende, Klaus Becker, Martina Wanis, Saiedeh Saghafi, Rashmit Kaur, Christian Hahn, Nika Pende, Massih Foroughipour, Thomas Hummel, Hans-Ulrich Dodt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/9a6600624b624c8a9084b66847032960
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spelling	oai:doaj.org-article:9a6600624b624c8a9084b668470329602021-12-02T17:32:14ZHigh-resolution ultramicroscopy of the developing and adult nervous system in optically cleared Drosophila melanogaster10.1038/s41467-018-07192-z2041-1723https://doaj.org/article/9a6600624b624c8a9084b668470329602018-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-07192-zhttps://doaj.org/toc/2041-1723Optical aberrations due to pigments in the eye and cuticle have undermined the ability for high-resolution imaging of the intact Drosophila. Here, the authors report an improved tissue-clearing agent, light-sheet optics and a multi-view combining algorithm to overcome these limitations.Marko PendeKlaus BeckerMartina WanisSaiedeh SaghafiRashmit KaurChristian HahnNika PendeMassih ForoughipourThomas HummelHans-Ulrich DodtNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Marko Pende Klaus Becker Martina Wanis Saiedeh Saghafi Rashmit Kaur Christian Hahn Nika Pende Massih Foroughipour Thomas Hummel Hans-Ulrich Dodt High-resolution ultramicroscopy of the developing and adult nervous system in optically cleared Drosophila melanogaster
description	Optical aberrations due to pigments in the eye and cuticle have undermined the ability for high-resolution imaging of the intact Drosophila. Here, the authors report an improved tissue-clearing agent, light-sheet optics and a multi-view combining algorithm to overcome these limitations.
format	article
author	Marko Pende Klaus Becker Martina Wanis Saiedeh Saghafi Rashmit Kaur Christian Hahn Nika Pende Massih Foroughipour Thomas Hummel Hans-Ulrich Dodt
author_facet	Marko Pende Klaus Becker Martina Wanis Saiedeh Saghafi Rashmit Kaur Christian Hahn Nika Pende Massih Foroughipour Thomas Hummel Hans-Ulrich Dodt
author_sort	Marko Pende
title	High-resolution ultramicroscopy of the developing and adult nervous system in optically cleared Drosophila melanogaster
title_short	High-resolution ultramicroscopy of the developing and adult nervous system in optically cleared Drosophila melanogaster
title_full	High-resolution ultramicroscopy of the developing and adult nervous system in optically cleared Drosophila melanogaster
title_fullStr	High-resolution ultramicroscopy of the developing and adult nervous system in optically cleared Drosophila melanogaster
title_full_unstemmed	High-resolution ultramicroscopy of the developing and adult nervous system in optically cleared Drosophila melanogaster
title_sort	high-resolution ultramicroscopy of the developing and adult nervous system in optically cleared drosophila melanogaster
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/9a6600624b624c8a9084b66847032960
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High-resolution ultramicroscopy of the developing and adult nervous system in optically cleared Drosophila melanogaster

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