The heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis

Abstract The heparan sulfate proteoglycan Syndecan-1, a mediator of signals between the extracellular matrix and cells involved is able to interact with OPG, one of the major regulators of osteoclastogenesis. The potential of osteoblasts to induce osteoclastogenesis is characterized by a switch of O...

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Autores principales:	Melanie Timmen, Heriburg Hidding, Martin Götte, Thaqif El Khassawna, Daniel Kronenberg, Richard Stange
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9a6ee4e2f41b4963b385a2e72b22d6ad
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spelling	oai:doaj.org-article:9a6ee4e2f41b4963b385a2e72b22d6ad2021-12-02T16:09:10ZThe heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis10.1038/s41598-020-77510-32045-2322https://doaj.org/article/9a6ee4e2f41b4963b385a2e72b22d6ad2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77510-3https://doaj.org/toc/2045-2322Abstract The heparan sulfate proteoglycan Syndecan-1, a mediator of signals between the extracellular matrix and cells involved is able to interact with OPG, one of the major regulators of osteoclastogenesis. The potential of osteoblasts to induce osteoclastogenesis is characterized by a switch of OPG (low osteoclastogenic potential) towards RANKL production (high osteoclastogenic potential). In the present study, we investigated the influence of endogenous Syndecan-1 on local bone-cell-communication via the RANKL/OPG-axis in murine osteoblasts and osteoclasts in wild type and Syndecan-1 lacking cells. Syndecan-1 expression and secretion was increased in osteoblasts with high osteoclastogenic potential. Syndecan-1 deficiency led to increased OPG release by osteoblasts that decreased the availability of RANKL. In co-cultures of Syndecan-1 deficient osteoblasts with osteoclast these increased OPG in supernatant caused decreased development of osteoclasts. Syndecan-1 and RANKL level were increased in serum of aged WT mice, whereas Syndecan-1 deficient mice showed high serum OPG concentration. However, bone structure of Syndecan-1 deficient mice was not different compared to wild type. In conclusion, Syndecan-1 could be regarded as a new modulator of bone-cell-communication via RANKL/OPG axis. This might be of high impact during bone regeneration or bone diseases like cancer where Syndecan-1 expression is known to be even more prevalent.Melanie TimmenHeriburg HiddingMartin GötteThaqif El KhassawnaDaniel KronenbergRichard StangeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Melanie Timmen Heriburg Hidding Martin Götte Thaqif El Khassawna Daniel Kronenberg Richard Stange The heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis
description	Abstract The heparan sulfate proteoglycan Syndecan-1, a mediator of signals between the extracellular matrix and cells involved is able to interact with OPG, one of the major regulators of osteoclastogenesis. The potential of osteoblasts to induce osteoclastogenesis is characterized by a switch of OPG (low osteoclastogenic potential) towards RANKL production (high osteoclastogenic potential). In the present study, we investigated the influence of endogenous Syndecan-1 on local bone-cell-communication via the RANKL/OPG-axis in murine osteoblasts and osteoclasts in wild type and Syndecan-1 lacking cells. Syndecan-1 expression and secretion was increased in osteoblasts with high osteoclastogenic potential. Syndecan-1 deficiency led to increased OPG release by osteoblasts that decreased the availability of RANKL. In co-cultures of Syndecan-1 deficient osteoblasts with osteoclast these increased OPG in supernatant caused decreased development of osteoclasts. Syndecan-1 and RANKL level were increased in serum of aged WT mice, whereas Syndecan-1 deficient mice showed high serum OPG concentration. However, bone structure of Syndecan-1 deficient mice was not different compared to wild type. In conclusion, Syndecan-1 could be regarded as a new modulator of bone-cell-communication via RANKL/OPG axis. This might be of high impact during bone regeneration or bone diseases like cancer where Syndecan-1 expression is known to be even more prevalent.
format	article
author	Melanie Timmen Heriburg Hidding Martin Götte Thaqif El Khassawna Daniel Kronenberg Richard Stange
author_facet	Melanie Timmen Heriburg Hidding Martin Götte Thaqif El Khassawna Daniel Kronenberg Richard Stange
author_sort	Melanie Timmen
title	The heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis
title_short	The heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis
title_full	The heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis
title_fullStr	The heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis
title_full_unstemmed	The heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis
title_sort	heparan sulfate proteoglycan syndecan-1 influences local bone cell communication via the rankl/opg axis
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/9a6ee4e2f41b4963b385a2e72b22d6ad
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The heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis

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