Novel evidence of HBV recombination in family cluster infections in western China.

Two hepatitis B virus (HBV) C/D recombinants were isolated from western China. No direct evidence indicates that these new viruses arose as a result of recombination between genotype C and D or a result of convergence. In this study, we search for evidence of intra-individual recombination in the fa...

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Autores principales:	Bin Zhou, Zhanhui Wang, Jie Yang, Jian Sun, Hua Li, Yasuhito Tanaka, Masashi Mizokami, Jinlin Hou
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9a71b9429b054d5988166f6e6455dab3
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spelling	oai:doaj.org-article:9a71b9429b054d5988166f6e6455dab32021-11-18T07:16:28ZNovel evidence of HBV recombination in family cluster infections in western China.1932-620310.1371/journal.pone.0038241https://doaj.org/article/9a71b9429b054d5988166f6e6455dab32012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22675528/?tool=EBIhttps://doaj.org/toc/1932-6203Two hepatitis B virus (HBV) C/D recombinants were isolated from western China. No direct evidence indicates that these new viruses arose as a result of recombination between genotype C and D or a result of convergence. In this study, we search for evidence of intra-individual recombination in the family cluster cases with co-circulation of genotype C, D and C/D recombinants. We studied 68 individuals from 15 families with HBV infections in 2006, identified individuals with mixed HBV genotype co-infections by restriction fragment length polymorphism and proceeded with cloning and DNA sequencing. Recombination signals were detected by RDP3 software and confirmed by split phylogenetic trees. Families with mixed HBV genotype co-infections were resampled in 2007. Three of 15 families had individuals with different HBV genotype co-infections in 2006. One individual (Y2) had a triple infection of HBV genotype C, D and C/D recombinant in 2006, but only genotype D in 2007. Further clonal analysis of this patient indicated that the C/D recombinant was not identical to previously isolated CD1 or CD2, but many novel recombinants with C2, D1 and CD1 were simultaneously found. All parental strains could recombine with each other to form new recombinant in this patient. This indicates that the detectable mixed infection and recombination have a limited time window. Also, as the recombinant nature of HBV precludes the possibility of a simple phylogenetic taxonomy, a new standard may be required for classifying HBV sequences.Bin ZhouZhanhui WangJie YangJian SunHua LiYasuhito TanakaMasashi MizokamiJinlin HouPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e38241 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bin Zhou Zhanhui Wang Jie Yang Jian Sun Hua Li Yasuhito Tanaka Masashi Mizokami Jinlin Hou Novel evidence of HBV recombination in family cluster infections in western China.
description	Two hepatitis B virus (HBV) C/D recombinants were isolated from western China. No direct evidence indicates that these new viruses arose as a result of recombination between genotype C and D or a result of convergence. In this study, we search for evidence of intra-individual recombination in the family cluster cases with co-circulation of genotype C, D and C/D recombinants. We studied 68 individuals from 15 families with HBV infections in 2006, identified individuals with mixed HBV genotype co-infections by restriction fragment length polymorphism and proceeded with cloning and DNA sequencing. Recombination signals were detected by RDP3 software and confirmed by split phylogenetic trees. Families with mixed HBV genotype co-infections were resampled in 2007. Three of 15 families had individuals with different HBV genotype co-infections in 2006. One individual (Y2) had a triple infection of HBV genotype C, D and C/D recombinant in 2006, but only genotype D in 2007. Further clonal analysis of this patient indicated that the C/D recombinant was not identical to previously isolated CD1 or CD2, but many novel recombinants with C2, D1 and CD1 were simultaneously found. All parental strains could recombine with each other to form new recombinant in this patient. This indicates that the detectable mixed infection and recombination have a limited time window. Also, as the recombinant nature of HBV precludes the possibility of a simple phylogenetic taxonomy, a new standard may be required for classifying HBV sequences.
format	article
author	Bin Zhou Zhanhui Wang Jie Yang Jian Sun Hua Li Yasuhito Tanaka Masashi Mizokami Jinlin Hou
author_facet	Bin Zhou Zhanhui Wang Jie Yang Jian Sun Hua Li Yasuhito Tanaka Masashi Mizokami Jinlin Hou
author_sort	Bin Zhou
title	Novel evidence of HBV recombination in family cluster infections in western China.
title_short	Novel evidence of HBV recombination in family cluster infections in western China.
title_full	Novel evidence of HBV recombination in family cluster infections in western China.
title_fullStr	Novel evidence of HBV recombination in family cluster infections in western China.
title_full_unstemmed	Novel evidence of HBV recombination in family cluster infections in western China.
title_sort	novel evidence of hbv recombination in family cluster infections in western china.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/9a71b9429b054d5988166f6e6455dab3
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Novel evidence of HBV recombination in family cluster infections in western China.

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